在线日韩日本国产亚洲丨少妇伦子伦情品无吗丨欧美性猛交xxxx免费看蜜桃丨精品人妻系列无码一区二区三区丨亚洲精品无码不卡在线播放

Your Good Partner in Biology Research

Human Ephrin type-A receptor 4(EPHA4) ELISA kit

  • 中文名稱:
    人A型肝配蛋白受體4(EPHA4)酶聯免疫試劑盒
  • 貨號:
    CSB-EL007724HU
  • 規格:
    96T/48T
  • 價格:
    ¥3600/¥2500
  • 其他:

產品詳情

  • 產品描述:
    人A型肝配蛋白受體4(EPHA4)酶聯免疫試劑盒(CSB-EL007724HU)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿、細胞裂解物樣本中的EPHA4含量。EPHA4是一個重要靶點。它屬于Eph受體家族,在神經系統發育中發揮關鍵作用,參與軸突導向、神經元遷移等過程。研究表明其異常激活或表達與腫瘤、神經系統疾病等多種病理機制相關,目前正探索通過抑制或調節它來開發針對性治療策略。試劑盒檢測范圍為25 pg/mL-1600 pg/mL,適用于基礎科研中探究EPHA4在神經系統疾病模型、腫瘤微環境調控或再生醫學領域的表達特征,亦可用于藥物干預實驗或信號通路研究中靶點蛋白的動態監測,為探索EPHA4在細胞通訊、疾病機制及治療靶點篩選中的功能研究提供可靠工具。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 別名:
    Cek 8 ELISA Kit; CEK8 ELISA Kit; EK8 ELISA Kit; eph receptor a4 ELISA Kit; EPH-like kinase 8 ELISA Kit; EPHA4 ELISA Kit; EPHA4_HUMAN ELISA Kit; Ephrin type-A receptor 4 ELISA Kit; HEK 8 ELISA Kit; hEK8 ELISA Kit; Receptor protein-tyrosine kinase HEK8 ELISA Kit; Sek 1 ELISA Kit; SEK ELISA Kit; TYRO 1 protein tyrosine kinase ELISA Kit; TYRO1 ELISA Kit; Tyrosine-protein kinase receptor SEK ELISA Kit; Tyrosine-protein kinase TYRO1 ELISA Kit
  • 縮寫:
  • Uniprot No.:
  • 種屬:
    Homo sapiens (Human)
  • 樣本類型:
    serum, plasma, tissue homogenates, cell lysates
  • 檢測范圍:
    25 pg/mL-1600 pg/mL
  • 靈敏度:
    6.25 pg/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Signal Transduction
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of human EPHA4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %93
    Range %90-95
    1:2Average %87
    Range %85-89
    1:4Average %87
    Range %82-90
    1:8Average %97
    Range %91-103
  • 回收率:
    The recovery of human EPHA4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 8985-92
    EDTA plasma (n=4)8785-90
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    16002.328 2.368 2.348 2.214
    8001.551 1.598 1.575 1.441
    4001.004 1.038 1.021 0.887
    2000.562 0.575 0.569 0.435
    1000.397 0.381 0.389 0.255
    500.265 0.278 0.272 0.138
    250.201 0.209 0.205 0.071
    00.135 0.132 0.134
  • 數據處理:
  • 貨期:
    3-5 working days

產品評價

相關問答

 常見問題解答
Q:

Per customers request, please provide the following information regarding Human Ephrin type-A receptor 4, EPHA4 ELISA Kit (CSB-EL007724HU)
a. what region of EphA4 are the antibodies raised against in the ELISA?
b. Customer is looking for an ELISA that can quantify the n-terminus/extracellular domain of human EPhA4 in serum/plasma

A:
Thanks for your inquiry!
CSB-EL007724HU
This kit is designed against extracellular domain. Pls refer to: https://www.uniprot.org/uniprot/P54764.
It is for quantitative determination of EPHA4. Sorry we are not clear about the antibody's recognition area.
Pls let me know if you have any further questions. Thank you.

靶點詳情

  • 功能:
    Receptor tyrosine kinase which binds membrane-bound ephrin family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Highly promiscuous, it has the unique property among Eph receptors to bind and to be physiologically activated by both GPI-anchored ephrin-A and transmembrane ephrin-B ligands including EFNA1 and EFNB3. Upon activation by ephrin ligands, modulates cell morphology and integrin-dependent cell adhesion through regulation of the Rac, Rap and Rho GTPases activity. Plays an important role in the development of the nervous system controlling different steps of axonal guidance including the establishment of the corticospinal projections. May also control the segregation of motor and sensory axons during neuromuscular circuit development. In addition to its role in axonal guidance plays a role in synaptic plasticity. Activated by EFNA1 phosphorylates CDK5 at 'Tyr-15' which in turn phosphorylates NGEF regulating RHOA and dendritic spine morphogenesis. In the nervous system, plays also a role in repair after injury preventing axonal regeneration and in angiogenesis playing a role in central nervous system vascular formation. Additionally, its promiscuity makes it available to participate in a variety of cell-cell signaling regulating for instance the development of the thymic epithelium. During development of the cochlear organ of Corti, regulates pillar cell separation by forming a ternary complex with ADAM10 and CADH1 which facilitates the cleavage of CADH1 by ADAM10 and disruption of adherens junctions.
  • 基因功能參考文獻:
    1. MiR-519d down-regulates EphA4 expression in melanoma. PMID: 29093007
    2. These findings confirmed that EphA4 is a direct target gene of miR-335 and that miR-335 suppresses breast cancer cell proliferation and motility in part by directly inhibiting EphA4 expression. PMID: 28795314
    3. Therefore EphA4 is an emerging AbetaOs receptor and the activation of the EphA4/c-Abl axis would explain the synaptic spine alterations found in Alzheimer's disease. PMID: 29378302
    4. These results demonstrate a novel role for SORLA as a physiological and pathological EphA4 modulator. PMID: 29114064
    5. The expression of both EphA4-FL and EphA4-N was significantly higher in the nervous tissue of SOD1(G93A) compared to wild-type mice suggesting that both forms are modulated during the disease process. PMID: 28153688
    6. the PI3K/AKT, Wnt/beta-catenin signaling pathways as well as ERK1/2 downstream of EPHA4 receptor activation, play an important role in the regulation of events related with the EMT development, which may be associated with the therapeutic failure in rectal cancer after radiotherapy. PMID: 27632701
    7. Molecular interactions of EphA4, growth hormone receptor, Jak2, and STAT5B have been described. PMID: 28686668
    8. Findings demonstrated that mutant alpha2-chimaerin and EphA4 have different genetic interactions in distinct motor neuron pools: abducens neurons use bidirectional ephrin signaling via mutant alpha2-chimaerin to direct growth, while cervical spinal neurons use only ephrin forward signaling PMID: 28346224
    9. Reduced EphA4 expression is associated with EBV-associated B lymphoma. PMID: 27338098
    10. No difference was found in the expression of EPHA4 in morphologically normal glands, HGPIN, or prostatic cancer. PMID: 27804940
    11. we supposed that EphA4 interacted with CDK5 and promoted its expression which in turn enhanced p-AKT expression and promoted cell adhesion-mediated drug resistance in multiple myeloma. PMID: 28351297
    12. EphA4 was reduced in breast carcinoma, which is associated with high grade, advanced TNM stage, lymph node metastasis, and poor outcome of patients PMID: 27478038
    13. Host EphA4 expression regulates cancer development mainly via EphA4-mediated IGF1 synthesis signal. PMID: 26923183
    14. EPHA4 is overexpressed but not functionally active in Sezary syndrome. PMID: 26376612
    15. The signaling complex appears to integrate the input from FGFR and EphA4, and release the output signal through FRS2alpha. PMID: 20184660
    16. EphA4 induced accumulation of amyloid precursor protein through a Lyn-mediated pathway. PMID: 24217950
    17. High Eph A4 expression is associated with choriocarcinoma invasion. PMID: 23429488
    18. The platelet P2Y12 receptor contributes to granule secretion through Ephrin A4 receptor. PMID: 22273509
    19. EphA4 gene expression is associated with an improved outcome in patients with resected lung adenocarcinoma, possibly by affecting cancer cell migration and invasion. PMID: 22807579
    20. Epha4 modulates the vulnerability of motor neurons to axonal degeneration and may represent a new target for therapeutic intervention in ALS. PMID: 22922411
    21. The present study demonstrates that EphA4 is expressed on neurons in multiple regions of the intact human brain and is markedly upregulated on activated astrocytes after TBI PMID: 22318127
    22. EphA4 expression maintains adult neural stem cells in an undifferentiated state. PMID: 21444754
    23. these results suggest that the ligand promiscuity of the Ephs is directly correlated with the structural flexibility of the ligand-binding surface of the receptor. PMID: 20678482
    24. Eph-A4 expression was significantly associated with tumor proliferative capacity in pancreatic ductal adenocarcinoma. PMID: 19949912
    25. Eph/ephrin signaling enhances the ability of platelet agonists to cause aggregation provided that those agonists can increase cytosolic Ca(++) and this is accomplished in part by activating Rap1 PMID: 14576067
    26. The tyrosine kinase receptor EphA4 and the potentially oncogenic transcription factor Twist were highly and selectively expressed in T cells of patients with Sezary Syndrome. PMID: 15313894
    27. EphA4 is physically associated with alpha(IIb)beta(3) in resting platelets, increases its surface expression when platelets are activated, and colocalizes with alpha(IIb)beta(3) at sites of contact between platelets. PMID: 15994237
    28. FGF-receptor-mediated mitogen-activated protein kinase stimulation is potentiated in cells costimulated with ephrin-A1 PMID: 16365308
    29. The expression of EphA4 in astrocyte progenitor cells and in the astrocyte meshwork at the optic nerve head has implications for optic nerve pathologies. PMID: 16574431
    30. Overexpression of EphA4 receptor is associated with pancreatic ductal adenocarcinoma PMID: 16965393
    31. overexpression of the EphA4 gene and reduced expression of the EphB2 gene might promote liver metastasis in colorectal cancer PMID: 18695888
    32. These results indicate that EphA4 plays an important role in malignant phenotypes of glioblastoma by enhancing cell proliferation and migration through accelerating a canonical FGFR signaling pathway. PMID: 18790757
    33. Overexpression of the receptor tyrosine kinase EphA4 in human gastric cancers. PMID: 18837080

    顯示更多

    收起更多

  • 亞細胞定位:
    Cell membrane; Single-pass type I membrane protein. Cell projection, axon. Cell projection, dendrite. Cell junction, synapse, postsynaptic density membrane. Early endosome. Cell junction, adherens junction.
  • 蛋白家族:
    Protein kinase superfamily, Tyr protein kinase family, Ephrin receptor subfamily
  • 組織特異性:
    Ubiquitous.
  • 數據庫鏈接:

    HGNC: 3388

    OMIM: 602188

    KEGG: hsa:2043

    STRING: 9606.ENSP00000281821

    UniGene: Hs.371218



主站蜘蛛池模板: 玖玖资源站无码专区| 中文文字幕文字幕亚洲色| 97超碰中文字幕久久精品| 国产高清在线精品二区| 综合人妻久久一区二区精品| 琪琪电影午夜理论片八戒八戒| 国产精品69人妻无码久久| 无码喷水一区二区浪潮av| 久久无码人妻影院| 精品丝袜人妻久久久久久| 亚洲中文字幕无码一去台湾| 亚洲愉拍二区一区三区| 久久av无码精品人妻系列试探| 美女裸体视频永久免费| 亚洲熟女乱综合一区二区在线| 日韩av无码中文无码电影| 中字乱码视频| 女人被狂躁c到高潮视频| 高清性欧美暴力猛交| 强制高潮18xxxxhd日韩| 99香蕉国产精品偷在线观看| 99久无码中文字幕一本久道| 无码中文字幕va精品影院| 内射人妻无码色ab麻豆| 亚洲日韩v无码中文字幕| 韩国无码av片在线观看| 亚洲乱码伦av| 久久久久亚洲精品无码网址| 亚洲人成电影网站色| 久久久精品人妻一区二区三区gv | 四虎影视在线观看2413| 最新精品国偷自产在线| 国产成人手机高清在线观看网站| 国产一区二区三区不卡在线看| 国产精品久久久久久久久ktv| 97免费公开在线视频| 欧美大胆作爱视频欣赏人体| 成人婷婷网色偷偷亚洲男人的天堂| 久热国产vs视频在线观看 | 国产成人免费ā片在线观看老同学| 日韩精品一区二区av在线|