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Human Ephrin-A1(EFNA1) ELISA kit

  • 中文名稱:
    人肝配蛋白A1受體(EFNA1)酶聯(lián)免疫試劑盒
  • 貨號(hào):
    CSB-EL007460HU
  • 規(guī)格:
    96T/48T
  • 價(jià)格:
    ¥3600/¥2500
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
    人肝配蛋白A1受體(EFNA1)酶聯(lián)免疫試劑盒(CSB-EL007460HU)為雙抗夾心法ELISA試劑盒,定量檢測(cè)血清、血漿、組織勻漿、細(xì)胞裂解物樣本中的EFNA1含量。EFNA1 即 EphrinA1,是 Eph 受體酪氨酸激酶的配體。它參與多種生理病理過程,如血管生成、細(xì)胞遷移等。研究機(jī)制方面,其通過與 Eph 受體相互作用,激活下游信號(hào)通路,影響細(xì)胞行為,在腫瘤、心血管疾病等研究中受關(guān)注。試劑盒檢測(cè)范圍為0.156 ng/mL-10 ng/mL,本產(chǎn)品為科研領(lǐng)域研究EFNA1在腫瘤侵襲轉(zhuǎn)移、血管異常增生或細(xì)胞遷移調(diào)控中的分子機(jī)制提供可靠工具,支持體外實(shí)驗(yàn)中對(duì)不同來源樣本的蛋白表達(dá)水平分析,廣泛應(yīng)用于癌癥生物學(xué)、細(xì)胞信號(hào)轉(zhuǎn)導(dǎo)及發(fā)育相關(guān)的基礎(chǔ)研究。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名:
    B61 ELISA Kit; ECKLG ELISA Kit; EFL 1 ELISA Kit; EFL1 ELISA Kit; EFNA 1 ELISA Kit; Efna1 ELISA Kit; EFNA1_HUMAN ELISA Kit; EPH related receptor tyrosine kinase ligand 1 ELISA Kit; EPH-related receptor tyrosine kinase ligand 1 ELISA Kit; Ephrin-A1 ELISA Kit; Ephrin-A1, secreted form ELISA Kit; EphrinA1 ELISA Kit; EPLG 1 ELISA Kit; EPLG1 ELISA Kit; Immediate early response protein B61 ELISA Kit; LERK 1 ELISA Kit; LERK-1 ELISA Kit; LERK1 ELISA Kit; Ligand of eph related kinase 1 ELISA Kit; OTTHUMP00000033242 ELISA Kit; OTTHUMP00000033271 ELISA Kit; secreted form ELISA Kit; TNF alpha-induced protein 4 ELISA Kit; TNFAIP 4 ELISA Kit; TNFAIP4 ELISA Kit; Tumor necrosis factor alpha induced protein 4 ELISA Kit; Tumor necrosis factor alpha-induced protein 4 ELISA Kit
  • 縮寫:
  • Uniprot No.:
  • 種屬:
    Homo sapiens (Human)
  • 樣本類型:
    serum, plasma, tissue homogenates, cell lysates
  • 檢測(cè)范圍:
    0.156 ng/mL-10 ng/mL
  • 靈敏度:
    0.039 ng/mL
  • 反應(yīng)時(shí)間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測(cè)波長(zhǎng):
    450 nm
  • 研究領(lǐng)域:
    Cardiovascular
  • 測(cè)定原理:
    quantitative
  • 測(cè)定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of Human EFNA1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %86
    Range %84-92
    1:2Average %96
    Range %90-102
    1:4Average %93
    Range %85-97
    1:8Average %88
    Range %82-94
  • 回收率:
    The recovery of Human EFNA1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9387-97
    EDTA plasma (n=4)8682-94
  • 標(biāo)準(zhǔn)曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    102.918 2.717 2.818 2.701
    52.425 2.524 2.475 2.358
    2.51.913 1.816 1.865 1.748
    1.251.130 1.182 1.156 1.039
    0.6250.580 0.547 0.564 0.447
    0.3120.345 0.331 0.338 0.221
    0.1560.207 0.211 0.209 0.092
    00.116 0.117 0.117
  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

引用文獻(xiàn)

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能:
    Cell surface GPI-bound ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. Plays an important role in angiogenesis and tumor neovascularization. The recruitment of VAV2, VAV3 and PI3-kinase p85 subunit by phosphorylated EPHA2 is critical for EFNA1-induced RAC1 GTPase activation and vascular endothelial cell migration and assembly. Exerts anti-oncogenic effects in tumor cells through activation and down-regulation of EPHA2. Activates EPHA2 by inducing tyrosine phosphorylation which leads to its internalization and degradation. Acts as a negative regulator in the tumorigenesis of gliomas by down-regulating EPHA2 and FAK. Can evoke collapse of embryonic neuronal growth cone and regulates dendritic spine morphogenesis.
  • 基因功能參考文獻(xiàn):
    1. ephrin-A1 seems to be remarkably involved in elementary processes of endothelial migration like cellular polarization, migratory direction and speed. PMID: 27742560
    2. Findings show that EFNA1 expression is a useful marker for predicting a high risk of recurrence in hepatocellular carcinoma patients but not EPHA2. PMID: 24969670
    3. Results suggest that EFNA1 is involved in colorectal tumorigenesis, and rs12904 A>G polymorphism in the 3' UTR of EFNA1 is associated with CRC susceptibility in a Chinese population. PMID: 24175772
    4. EphA2-ephrinA1 trans-endocytosis is sensitive to the mechanical properties of a cell's microenvironment PMID: 24853748
    5. Ephrin-A1 is upregulated in tumor microenvironment and promotes angiogenesis through a coordinated cross-talk with PI3K/Akt-dependent eNOS activation. PMID: 24040255
    6. The interaction between ephrin-As, Eph receptors and integrin alpha3 is plausibly important for the crosstalk between Eph and integrin signalling pathways at the membrane protrusions and in the migration of brain cancer cells. PMID: 23686814
    7. Increased expression of EFNA1 mRNA is associated with gastric cancer. PMID: 23065816
    8. Expression of EPHRIN-A1 tends to be associated with worse survival in head and neck cancer. PMID: 24330498
    9. EFNA1 expression is a useful marker for predicting high risk of relapse and cancer-related death in patients who have undergone curative resection for CRC. PMID: 23258614
    10. Findings suggest that the glycosylation on ephrin-A1 plays a critical role in the binding and activation of the EphA2 receptor. PMID: 23661698
    11. present study showed a high expression of EphA2/ephrinA1 in adenoid cystic carcinoma. EphA2/ephrinA1 can serve as a novel therapy target for adenoid cystic carcinoma. PMID: 23298804
    12. EphA2 activation by ephrin-A1 induces tumor suppressor gene cdx-2 expression which attenuates cell proliferation, tumor growth and thus may be a promising therapeutic target against NSCLC. PMID: 22824143
    13. in p-stage I NSCLC patients, those in the higher EphA2 expression and higher ephrin-A1 expression groups shared almost the same clinicopathological backgrounds which are generally considered to be better prognostic factors. PMID: 22236865
    14. The EphA2 ligand EphrinA1 induces EphA2 phosphorylation and intracellular internalization and degradation, thus inhibiting breast tumor progression. PMID: 22228563
    15. Multiple oncogenic signalling pathways are affected by ephrin-A1, from the promotion of a specific pathway in one cell or cancer type to the inhibition of the same pathway in another type of cell or cancer. [Review] PMID: 22040911
    16. Ovarian serous carcinomas and ovarian cancer cell lines overexpress EphA2 and EphrinA-1. Tumor patients with higher expression levels of both EphA2 and EphrinA-1 have a significantly poorer clinical outcome. PMID: 21500549
    17. The Eph-ephrinA system can promote cell attachment along with intercellular dissociation. PMID: 21349856
    18. This study demonstrated that the Eph-ephrin A system can promote intercellular dissociation in Ishikawa cells suggesting an important role in the initial step of embryo implantation by opening the endometrial epithelial cell barrier. PMID: 21138904
    19. Osteosarcoma samples were characterized using genome-wide microarrays: increased expression of the EphA2 receptor and its ligand EFNA1 was detected. PMID: 21166698
    20. up-regulation of EphA2 and down-regulation of Ephrina1 may correlate with poor prognosis for patients with high-grade glioma PMID: 20571968
    21. EphA2 and EphrinA1 are highly expressed in renal cell carcinoma, and positively correlated with histological differentiation, clinical stage and angiogenesis. PMID: 19950554
    22. EFNA1 may be a useful serum marker for the detection of hepatocellular carinoma development and progression. PMID: 19642143
    23. The interaction between EphA2 and this ligand protein is necessary for induction of maximal neovascularization by VEGF. PMID: 12496364
    24. This protein, an EphA ligand, stimulated protein degradation by EphA2. PMID: 12496371
    25. Ephrin-A1 stimulation of Jurkat T cells induces tyrosine phosphorylation of EphA3 receptors and cytoplasmic proteins, including c-cbl proto-oncogene, and causes down-regulation of endogenous EphA3 receptors from the cell surface and their degradation. PMID: 12794130
    26. A new ephrin-A1 isoform, ephrin-A1b, lacks a segment of 22 amino acids (residues 131-152). Exon 3 is spliced out in its transcript. It may regulate the function of its ephrin-A1a counterpart. PMID: 14692877
    27. This study demonstrates for the first time significantly reduced ephrin-A1 expression in T cells of asthma patients. PMID: 14707054
    28. found ephrin-A1 expressed exclusively in the invasive extravillous trophoblast in preeclampsia and normal placenta PMID: 15193868
    29. ephrin-A1 is expressed by venule endothelial cells PMID: 15585656
    30. EPHA2 and EFNA1 expression may influence the behavior of human gastric cancer. PMID: 15649254
    31. low molecular weight protein-tyrosine phosphatase acts as terminator of EphA2 signaling causing efficient negative feedback loop on biological response mediated by ephrinA1; tyrosine phosphorylation main event orchestrating repulsive response PMID: 16051609
    32. High expression of Ephrin A-1 is associated with urinary bladder carcinoma. PMID: 16428472
    33. Activation of ERK-1/2 plays an essential role in ephrin-A1-mediated cell migration, whixh is inhibited by green tea catechin epigallocatechin gallate. PMID: 17049832
    34. Ephrin-A1 serves as a critical negative regulator in the tumorigenesis of gliomas by down-regulating EphA2 and FAK. PMID: 17332925
    35. Increasing ephrin-A expression enhances T-cell interactions not only with purified integrin ligands but also endothelial cells, while EphA activation down-regulates these interactions. PMID: 17980912
    36. Soluble monmomeric EphrinA1 is released from tumor cells and is a functional ligand for the EphA2 receptor. PMID: 18794797
    37. The function of EphA2 and ephrinA1 in tumorigenesis and tumor progression is complex and seems to be dependent on cell type and microenvironment PMID: 19074825
    38. Increased expression of EphA2 and EphrinA-1 plays an important role in the progression human gastric adenocarcinoma. PMID: 19101799
    39. The crystal structures of an A-class complex between EphA2 and ephrin-A1 and of unbound EphA2, are presented. PMID: 19525919

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  • 亞細(xì)胞定位:
    Cell membrane; Lipid-anchor, GPI-anchor.; [Ephrin-A1, secreted form]: Secreted.
  • 蛋白家族:
    Ephrin family
  • 組織特異性:
    Brain. Down-regulated in primary glioma tissues compared to the normal tissues. The soluble monomeric form is expressed in the glioblastoma multiforme (GBM) and breast cancer cells (at protein level).
  • 數(shù)據(jù)庫(kù)鏈接:

    HGNC: 3221

    OMIM: 191164

    KEGG: hsa:1942

    STRING: 9606.ENSP00000357392

    UniGene: Hs.516664



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