在线日韩日本国产亚洲丨少妇伦子伦情品无吗丨欧美性猛交xxxx免费看蜜桃丨精品人妻系列无码一区二区三区丨亚洲精品无码不卡在线播放

Your Good Partner in Biology Research

Human ELAV-like protein 1(ELAVL1) ELISA kit

  • 中文名稱:
    人ELAVL1樣蛋白1(ELAVL1)酶聯免疫試劑盒
  • 貨號:
    CSB-EL007592HU
  • 規格:
    96T/48T
  • 價格:
    ¥3600/¥2500
  • 其他:

產品詳情

  • 產品描述:
    人ELAVL1樣蛋白1(ELAVL1)酶聯免疫試劑盒(CSB-EL007592HU)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿、細胞裂解物樣本中的ELAVL1含量。ELAVL1是一種RNA結合蛋白,在細胞生長、分化等生理過程中發揮重要作用。研究發現它通過與特定RNA結合,調控其穩定性、轉運和翻譯等。其異常表達與多種疾病相關,如腫瘤發生發展,目前針對它的作用機制研究是熱點,有望為相關疾病治療提供新靶點。試劑盒檢測范圍為25 pg/mL-1600 pg/mL,該產品可廣泛應用于分子生物學研究領域,如探索ELAVL1在腫瘤細胞信號通路中的調控機制、評估特定處理條件下蛋白表達水平變化,或用于神經退行性疾病模型中RNA結合蛋白的功能研究,為科研人員提供穩定可靠的定量分析工具。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 別名:
    ELAV (embryonic lethal abnormal vision Drosophila) like 1 ELISA Kit; ELAV (embryonic lethal; abnormal vision; Drosophila) like 1 (Hu antigen R) ELISA Kit; ELAV like 1 ELISA Kit; ELAV like RNA binding protein 1 ELISA Kit; ELAV-like protein 1 ELISA Kit; ELAV1 ELISA Kit; ELAV1_HUMAN ELISA Kit; Elavl1 ELISA Kit; Embryonic lethal abnormal vision drosophila homolog like 1 ELISA Kit; Hu Antigen R ELISA Kit; Hu-antigen R ELISA Kit; Hua ELISA Kit; HuR ELISA Kit; MelG ELISA Kit
  • 縮寫:
  • Uniprot No.:
  • 種屬:
    Homo sapiens (Human)
  • 樣本類型:
    serum, plasma, tissue homogenates, cell lysates
  • 檢測范圍:
    25 pg/mL-1600 pg/mL
  • 靈敏度:
    6.25 pg/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Epigenetics and Nuclear Signaling
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of human ELAVL1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)
    1:1 Average % 95
    Range % 89-99
    1:2 Average % 96
    Range % 92-101
    1:4 Average % 94
    Range % 88-98
    1:8 Average % 92
    Range % 88-96
  • 回收率:
    The recovery of human ELAVL1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 98 94-102
    EDTA plasma (n=4) 87 83-92
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    1600 2.167 2.058 2.113 1.956
    800 1.643 1.553 1.598 1.441
    400 1.166 1.137 1.152 0.995
    200 0.787 0.759 0.773 0.616
    100 0.542 0.536 0.539 0.382
    50 0.368 0.352 0.360 0.203
    25 0.279 0.271 0.275 0.118
    0 0.158 0.156 0.157  
  • 數據處理:
  • 貨期:
    3-5 working days

產品評價

靶點詳情

  • 功能:
    RNA-binding protein that binds to the 3'-UTR region of mRNAs and increases their stability. Involved in embryonic stem cells (ESCs) differentiation: preferentially binds mRNAs that are not methylated by N6-methyladenosine (m6A), stabilizing them, promoting ESCs differentiation. Binds to poly-U elements and AU-rich elements (AREs) in the 3'-UTR of target mRNAs. Binds avidly to the AU-rich element in FOS and IL3/interleukin-3 mRNAs. In the case of the FOS AU-rich element, binds to a core element of 27 nucleotides that contain AUUUA, AUUUUA, and AUUUUUA motifs. Binds preferentially to the 5'-UUUU[AG]UUU-3' motif in vitro. With ZNF385A, binds the 3'-UTR of p53/TP53 mRNA to control their nuclear export induced by CDKN2A. Hence, may regulate p53/TP53 expression and mediate in part the CDKN2A anti-proliferative activity. May also bind with ZNF385A the CCNB1 mRNA. Increases the stability of the leptin mRNA harboring an AU-rich element (ARE) in its 3' UTR.
  • 基因功能參考文獻:
    1. B4GALT1-AS1 recruited HuR to enhance YAP mRNA stability and thus its transcriptional activity PMID: 30182452
    2. HuR associates with TERC in vitro and in cultured cells. The association of HuR with TERC was required for the maintenance of TERC methylation and hence telomerase activity. Additionally, the regulation of telomerase activity by HuR was found to impact on the renewal of hematopoietic stem cells and was linked to dyskeratosis congenita, aplastic anemia, and autosomal dominant dyskeratosis congenita. PMID: 29880812
    3. this study identified an essential Jun/miR-22/HuR regulatory axis in CRC (the working model is summarised in Fig. 8) and highlighted the vital role of HuR and miR-22 in CRC proliferation and migration. PMID: 29351796
    4. RNA immunoprecipitation and RNA pull-down assays in glioma cell lines demonstrated that SNHG12 was associated with and was stabilized by Hu antigen R. Glioma patients with high levels of SNHG12 exhibited a reduced 5-year overall survival rate. PMID: 30015836
    5. Knockdown of HuR significantly increases the apoptosis of airway epithelial cells and down-regulates ZEB-1 expression. PMID: 29866111
    6. High HUR expression is associated with Invasive Breast Carcinoma. PMID: 28808873
    7. HuR acts as a tumor promoter by enhancing YAP expression in osteosarcoma cells. PMID: 29597092
    8. Study supports HuR's role as an upstream regulator of p62 expression in ARPE-19 cells, helps to understand better the early events in response to a proautophagy stimulus. PMID: 29576851
    9. Our data...strongly suggest that HuR expression...could be used as a prognostic marker in melanoma. PMID: 29393978
    10. HuR protein is expressed in the majority of the cases in all the tumors of the head and neck region examined. More importantly, higher levels of HuR expression have been noted, showing higher cytoplasmic levels in malignant or premalignant cases. Moreover, HuR modulates several genes implicated in biological processes important for malignant transformation, growth, and invasiveness. [review] PMID: 29619127
    11. Radiation-activated ataxia-telangiectasia mutated kinase/p38 signaling positively contributed to the nucleus to cytosol translocation of HuR. PMID: 29856906
    12. that HuR is involved in the process of acquiring malignant histopathological features and ability to invade the muscularis propria by urothelial bladder cancer cells PMID: 29566946
    13. HuR facilitated lung cancer stemness dependent on CDK3 expression. miR-873 or miR-125a-3p level was negatively correlated with HuR and CDK3 expression levels in lung cancer tissues. HuR facilitates lung cancer stemness via regulating miR-873/CDK3 and miR-125a-3p/CDK3 axis. PMID: 29344850
    14. HuR plays an important role in the progression of esophageal carcinoma by targeting IL-18. PMID: 28231690
    15. the promotive effects of HuR overexpression on the inflammatory response were attenuated when HUVECs were cotreated with HMGB1 short hairpin RNA. Therefore, the present results indicated that the ectopic expression of HuR may induce inflammatory responses and thus sepsis by activating the HMGB1 signaling pathway. PMID: 29115544
    16. Computational analysis of publicly available microarray datasets of non-small cell lung cancer (NSCC) treated with EGFR targeted therapy identified ESR1 and ELAV1 as the most highly ranked hub genes involved in the NSCLC acquired TKI-resistance. PMID: 29758295
    17. Studies indicate that by regulating the mRNAs of target genes, Hu antigen R protein (HuR) is causally linked to the onset and progression of kidney diseases [Review]. PMID: 28910975
    18. The widespread distribution of intronic adenylate-uridylate (AU)-rich elements (AREs) and their particular association with ELAVL1/HuR and ELAVL1/HuR binding sites indicates that more than half of human genes can be regulated post-transcriptionally by AREs. PMID: 29413897
    19. Posttranscriptional regulation of HuR by miR-133b enhances DTX cytotoxicity through inhibition of ABCG2. PMID: 29327946
    20. HuR is required for pancreatic ductal adenocarcinoma tumor growth. PMID: 28242812
    21. Transgenic expression of HuR in astrocytes accentuated neuronal injury and other secondary features of spinal cord injury including increased vascular permeability and astrocyte activation. PMID: 28487080
    22. HuR may be involved in the pathogenesis of glaucoma. PMID: 29107807
    23. Findings reveal a new mechanism whereby HUR protects NONO from mir320-mediated degradation upon UVC exposure and identify a new component within the complex network of players underlying the DNA damage response adding mir320a to the list of p53-regulated targets upon genotoxic stress. PMID: 27816966
    24. ectopic expression of HuR promotes breast cancer cell proliferation and survival by directly binding to and stabilizing CDK3 mRNA. PMID: 28501005
    25. New ELAVL1-TYK2 fusion gene is oncogenic in acute myeloid leukemia. PMID: 27189703
    26. High expression of HuR is correlated with thyroid cancer. PMID: 27542231
    27. Folate deficiency impaired spermatogenesis and reduced sperm concentration may partly due to inhibiting the expression of the three key molecules (Esr1, Cav1 and Elavl1) essential for sperm production. PMID: 28445960
    28. these studies demonstrate that HuR knockdown in triple-negative breast cancer cells elicits oxidative stress and DNA damage resulting in radiosensitization PMID: 27588488
    29. LncRNA-AK058003 can reduce the expression of mRNA stabilizing protein HuR and act as a precursor of miR-15a to suppress gamma-synuclein-mediated cell proliferation and the metastasis of hepatocellular carcinoma. PMID: 28035067
    30. These results indicate that HuR plays an important role in adenovirus replication, at least in part, by up-regulating IVa2 mRNA expression and that AU-rich element (ARE)-mRNA stabilization is required for both transformation and virus replication. PMID: 29225167
    31. Data indicate that both ELAVL1 and TIAR positively regulate endogenous SNCA in vivo. PMID: 29149290
    32. identified HuR as a novel posttranscriptional regulator of ABCA1 expression and cellular cholesterol homeostasis, thereby opening new avenues for increasing cholesterol efflux from atherosclerotic foam macrophages and raising circulat-ing HDL cholesterol levels. PMID: 24729624
    33. HuR expression and cytoplasmic localization were increased in fibrotic livers. S1P induced migration of human bone marrow Mesenchymal Stem Cells via S1PR3 and HuR. PMID: 27543493
    34. RNA-binding protein HuR (HuR) expression negatively correlated with chemokine (CC motif) ligand 5 (CCL5) expression and macrophage appearance in a cohort of breast tumors. PMID: 28731284
    35. Data indicate no correlation between RNA-binding protein HuR intracellular localization in tumor sections and progression free survival. PMID: 26943573
    36. our research demonstrated that NEAT1 acts as a key regulator in human ovarian carcinogenesis and revealed two regulatory factors (HuR and miR-124-3p) of NEAT1 expression. PMID: 27075229
    37. The crucial role of HuR as a stabilizing factor for the MR transcript. PMID: 28744670
    38. study found cytoplasmic HuR is significantly overexpressed in higher-grade (atypical and anaplastic) than in lower-grade meningiomas and the HuR level is correlated with survival in patients with meningioma; siHuR-induced HuR knockdown was shown to reduce the growth of both Ben-Men-1 and IOMM-Lee cells PMID: 28493484
    39. combined treatments of siHuR and epirubicin significantly reduced the expression of Bcl-2, but increased the expression of Bax, as well as activity and expression levels of caspase-3 and -9. In contrast, overHuR abrogated these effects PMID: 28968471
    40. High HuR expression is associated with pancreatic ductal adenocarcinomas. PMID: 28687616
    41. GPRC5A is a potential oncogene in pancreatic ductal adenocarcinoma cells that is upregulated by gemcitabine with help from HuR. PMID: 27415424
    42. Hur role in the epithelial-to-mesenchymal transition in pancreatic cancer PMID: 28965949
    43. exposing glioma cells to the HuR inhibitor tanshinone group compound 15,16-dihydrotanshinone-I or to the newly identified compound 5 disrupts HuR multimerization modules and reduces tumor cell survival and proliferation. PMID: 28790173
    44. We propose that the extensive binding of CircPABPN1 to HuR prevents HuR binding to PABPN1 mRNA and lowers PABPN1 translation, providing the first example of competition between a circRNA and its cognate mRNA for an RBP that affects translation. PMID: 28080204
    45. HuR contributes to efficient oxidative phosphorylation by regulating of CoQ10 biosynthesis. PMID: 26690054
    46. Data (including data from studies using knockout mice or cells from knockout mice) suggest HUR post-transcriptionally regulates CCR6 expression by binding to and stabilizing CCR6 mRNA and by promoting CCR6 translation in helper T-cells; knock-out of HUR reduces CCR6 expression on helper T-cells, impairs their migration to CNS, and prevents experimental autoimmune encephalomyelitis. (CCR6 = C-C chemokine receptor type 6) PMID: 28684423
    47. Taken together, our results indicate that HuR activation is an early event in familial adenomatosis polyposis (FAP)--adenoma but is not present in inflammatory bowel disease (IBD)-dysplasia. Furthermore, our results offer a preclinical proof of concept for HuR inhibition as an effective means of FAP chemoprevention, with caution advised in the setting of IBD PMID: 28428272
    48. Negative regulation of RNA-binding protein HuR by tumor-suppressor ECRG2 PMID: 26434587
    49. Data suggest that ERBB2 is post-transcriptionally up-regulated in tamoxifen-resistant estrogen receptor-positive breast cancer cells; ERBB2 expression is decreased post-transcriptionally by miRNA26a/b binding to its 3prime- untranslated region; ERBB2 expression is increased post-transcriptionally by HuR binding to its 3prime-untranslated region. (ERBB2 = epidermal growth factor receptor 2; HuR = Hu antigen R) PMID: 28637868
    50. by modulating extracellular export of miR-122, HuR could control stress response in starved human hepatic cells. PMID: 27402548

    顯示更多

    收起更多

  • 亞細胞定位:
    Cytoplasm. Nucleus. Cytoplasm, Stress granule.
  • 蛋白家族:
    RRM elav family
  • 組織特異性:
    Ubiquitous. Detected in brain, liver, thymus and muscle.
  • 數據庫鏈接:

    HGNC: 3312

    OMIM: 603466

    KEGG: hsa:1994

    STRING: 9606.ENSP00000385269

    UniGene: Hs.184492



主站蜘蛛池模板: 欧美性白人极品hd| 日韩不卡1卡2卡三卡2021精品推荐| 午夜福利啪啪片| av在线观看地址| 色先锋资源久久综合5566| 久久国产午夜精品理论片| 久久国产av影片| 97色偷偷色噜噜狠狠爱网站| 狠狠躁日日躁夜夜躁2020| 亚洲综合精品成人| 精品 在线 视频 亚洲| 无码av高潮抽搐流白浆| 2020每日更新国产精品视频| 久久久亚洲欧洲日产国码二区 | 国产成人一区二区青青草原| 99久久99久久精品免费看蜜桃| 人妻另类 专区 欧美 制服| 成人av在线一区二区三区| 欧美成 人 网 站 免费| 色橹橹欧美在线观看视频高清| 成片免费观看视频大全| 大香伊蕉日本一区二区| 亚洲色成人www永久在线观看| 麻豆精产国品| 中文字幕久久久久人妻中出| 高清国产在线拍揄自揄视频| 国产精品一区二区久久国产| 狠狠色丁香婷婷久久综合| 国产成+人+综合+亚洲 欧美| 精品无码国产自产拍在线观看 | 久久无码av中文出轨人妻| 在线看片免费人成视频影院看| 永久免费看啪啪网址入口| 亚洲精品国偷拍自产在线观看蜜臀| 久久综合九色综合97婷婷| 最新 国产 精品 精品 视频| 亚洲一区二区三区影院| 成人免费视频在线观看| 国产成人无码av大片大片在线观看 | 国产av天堂亚洲国产av天堂| 亚洲香蕉成人av网站在线观看|