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  4. Human E3 ubiquitin-protein ligase TRIM63(TRIM63) ELISA kit

Human E3 ubiquitin-protein ligase TRIM63(TRIM63) ELISA kit

  • 中文名稱：
    人E3泛素蛋白連接酶TRIM63(TRIM63)酶聯免疫試劑盒
  • 貨號：
    CSB-EL024502HU
  • 規格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產品詳情

  • 產品描述：
    人E3泛素蛋白連接酶TRIM63(TRIM63)酶聯免疫試劑盒（CSB-EL024502HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細胞裂解物樣本中的TRIM63含量。TRIM63也稱MuRF1，是肌肉特異性泛素連接酶。其背景在于與肌肉萎縮密切相關。研究機制方面，它能識別并泛素化特定底物蛋白，促使其通過蛋白酶體途徑降解，在肌肉蛋白穩態維持、肌肉萎縮調控等方面發揮重要作用。試劑盒檢測范圍為12.5 pg/mL-800 pg/mL，該產品適用于肌肉生物學、運動醫學及代謝性疾病等基礎研究領域，可探索TRIM63在骨骼肌再生、心肌肥大或腫瘤微環境中的功能機制，為體外模型、動物實驗或人類組織樣本的蛋白表達分析提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    E3 ubiquitin-protein ligase TRIM63 ELISA Kit; FLJ32380 ELISA Kit; IRF ELISA Kit; Iris RING finger protein ELISA Kit; MURF 1 ELISA Kit; MURF-1 ELISA Kit; MuRF1 ELISA Kit; MURF2 ELISA Kit; Muscle specific ring finger protein 1 ELISA Kit; Muscle specific ring finger protein 2 ELISA Kit; Muscle-specific RING finger protein 1 ELISA Kit; OTTHUMP00000008701 ELISA Kit; RING finger protein 28 ELISA Kit; RNF 28 ELISA Kit; RNF28 ELISA Kit; SMRZ ELISA Kit; Striated muscle RING zinc finger protein ELISA Kit; TRI63_HUMAN ELISA Kit; TRIM 63 ELISA Kit; Trim63 ELISA Kit; Tripartite motif containing 63 ELISA Kit; tripartite motif containing 63, E3 ubiquitin protein ligase ELISA Kit; Tripartite motif containing protein 63 ELISA Kit; Tripartite motif-containing protein 63 ELISA Kit; Ubiquitin ligase TRIM63 ELISA Kit
  • 縮寫：
    TRIM63
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates, cell lysates
  • 檢測范圍：
    12.5 pg/mL-800 pg/mL
  • 靈敏度：
    3.1 pg/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Cell Biology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human TRIM63 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:5Average %91
    Range %87-95
    1:10Average %97
    Range %93-101
    1:20Average %102
    Range %99-104
    1:40Average %88
    Range %86-90
  • 回收率：
    The recovery of human TRIM63 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9086-94
    EDTA plasma (n=4)10298-106
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    8002.508 2.725 2.617 2.430
    4002.013 2.112 2.063 1.876
    2001.337 1.325 1.331 1.144
    1000.825 0.827 0.826 0.639
    500.474 0.440 0.457 0.270
    250.339 0.330 0.335 0.148
    12.50.271 0.282 0.277 0.090
    00.184 0.189 0.187
  • 數據處理：
  • 貨期：
    3-5 working days

產品評價

靶點詳情

  • 功能：
    E3 ubiquitin ligase. Mediates the ubiquitination and subsequent proteasomal degradation of CKM, GMEB1 and HIBADH. Regulates the proteasomal degradation of muscle proteins under amino acid starvation, where muscle protein is catabolized to provide other organs with amino acids. Inhibits de novo skeletal muscle protein synthesis under amino acid starvation. Regulates proteasomal degradation of cardiac troponin I/TNNI3 and probably of other sarcomeric-associated proteins. May play a role in striated muscle atrophy and hypertrophy by regulating an anti-hypertrophic PKC-mediated signaling pathway. May regulate the organization of myofibrils through TTN in muscle cells.
  • 基因功能參考文獻：
    1. role of the muscle specific E3s MuRF-1 and MAFbx in skeletal muscle wasting during various pathologies, as well as their regulation by modifiable lifestyle factors, were explored (review) PMID: 26738803
    2. the involvement of oxidative stress in the atrophy of COPD peripheral muscle cells in vitro, via the FoxO1/MuRF1/atrogin-1 signaling pathway of the ubiquitin/proteasome system PMID: 27526027
    3. The mitochondrial damage-cGAS-STING-IRF3 pathway is critically involved in metabolic stress-induced endothelial inflammation. PMID: 28302626
    4. Altogether, these results suggest a novel function for p63 as a contributor to muscular atrophic processes via the regulation of multiple genes, including the muscle atrophy gene Trim63. PMID: 26919175
    5. Vitamin D3 might have an inhibitory effect on the expression of MAFbx and MuRF1 in skeletal muscle. PMID: 25876656
    6. MURF1 expression intended to be increased in the skeletal muscle of patients with malignant disease even before cancer related cachexia weight loss. PMID: 25760630
    7. TRIM63 gene expression involved in skin hyperpigmentation. PMID: 25950827
    8. Expression of USP19 correlates with that of MuRF1 and MAFbx/atrogin-1 in skeletal muscles PMID: 26048142
    9. Skeletal muscle atrophy induced by Angiotensin II involves activation of MuRF1 expression. PMID: 26137861
    10. These data strongly supported that rare variants in MuRF1 and MuRF2 are associated with higher penetrance and more severe clinical manifestations of hypertrophic cardiomyopathy. PMID: 24865491
    11. In conclusion, atrogin-1, MuRF1, FOXO1/3A, and eIF3-f mRNA, and protein levels, are differentially regulated by exercise contraction mode but not WPH supplementation combined with hypertrophy-inducing training. PMID: 24458747
    12. Data reveal that Titin protein is a pseudokinase with non-detectable catalytic output but is a high-affinity binding locus for MuRF1. PMID: 24850911
    13. both MuRF1 and MAFbx are enriched in skeletal, cardiac, and smooth muscle--REVIEW PMID: 25096180
    14. SMAD3 regulates transcription of MuRF-1 by increasing FoxO3 binding at a conserved FRE-SBE motif within the proximal promoter region, and by increasing FoxO3 protein content and transcriptional activity. PMID: 24920680
    15. In MuRF1 the COS-box mediates the in vivo targeting of sarcoskeletal structures and points to the pharmacological relevance of the COS domain for treating MuRF1-mediated muscle atrophy. PMID: 24671946
    16. MURF-1 protein gene expression is increased in patients with severe burn injury. PMID: 23816995
    17. Quadriceps muscle MuRF-1 levels did not differ between patients with COPD (with normal or low fat-free mass index) and controls. MURF1 levels were not associated with quadriceps fiber cross-sectional area or strength in patients. PMID: 23844868
    18. Regular exercise training leads to a decrease in Rnf28 expression in skeletal muscle in patients with advanced chronic heart failure. PMID: 22445192
    19. relationship was found between IL-6 and MuRF-1 expression after incubation with PGE2 PMID: 23490068
    20. MuRF-1 RNA expression was significantly increased in malnourished cirrhotic patients vs. well-nourished patients. PMID: 23432902
    21. Data suggest that expression of atrogin-1 and MuRF-1 likely play role in aging-related decrease in muscle mass (i.e., development of sarcopenia); up-regulation of atrogin-1 and MuRF-1 has potential to prevent or reverse sarcopenia. [REVIEW] PMID: 22815045
    22. Human molecular genetic and functional studies identify TRIM63, encoding Muscle RING Finger Protein 1, as a novel gene for human hypertrophic cardiomyopathy. PMID: 22821932
    23. investigation of factors regulating expression of two ubiquitin ligases (MURF1 and MAFbx) in skeletal muscle (i.e., vastus lateralis): effects of resistance exercise and anabolic dietary supplement (i.e., branched-chain amino acids) PMID: 22127230
    24. Data suggest that the inhibition of MuRF1 could be a novel mechanism to prevent or reverse muscle wasting associated with various pathologies. PMID: 21448668
    25. MuRF1 regulates cardiomyocyte cell size and energy metabolism to inhibit cardiac hypertrophy and reverse experimental cardiac hypertrophy. PMID: 21686210
    26. 11beta-HSD1 controls glucocorticoid-induced protein degradation in human and murine skeletal muscle via regulation of the E3 ubiquitin ligases Atrogin-1 and MuRF-1. PMID: 21304964
    27. Reduced expression of MuRF1 and MAFbx in the myocardium might permit hypertrophy characteristic of the early post-infarction remodeling phase. PMID: 19859778
    28. atrogin-1 specifically targets truncated M7t-cMyBP-C, but not WT-cMyBP-C, for proteasomal degradation and that MuRF1 indirectly reduces cMyBP-C levels by regulating the transcription of myosin heavy chain. PMID: 19850579
    29. interacts with titin to regulate sarcomeric M-line and thick filament structure and may have nuclear functions via its interaction with glucocorticoid modulatory element binding protein-1. PMID: 11927605
    30. MURF2 associates transiently with microtubules, myosin and titin during sarcomere assembly. PMID: 12414993
    31. MuRF1 functions as a ubiquitin ligase to catalyze ubiquitylation of troponin I through a RING finger-dependent mechanism PMID: 15601779
    32. MuRF1 mRNA expression was significantly increased in quadriceps of patients with COPD; transcriptional regulation of atrogin-1 and MuRF1 may occur via FoxO-1, but independently of AKT PMID: 17478621
    33. Expression of mRNA for MuRF-1 increased approximately 3-fold at 10 days without changes in MAFbx or tripeptidyl peptidase II mRNA, but all decreased between 10 and 21 days of muscle disuse. PMID: 17901116
    34. MuRF-1 and MAFbx, are differently affected by the exercise as well as by repeated exercise PMID: 17971512
    35. Results showed upregulation of MuRf1 and MAFbx in atrophied muscle and support their role as regulatory peptides in various conditions which lead to muscle atrophy. PMID: 17977773
    36. MuRF1 expression in skeletal muscle re-directs glycogen synthesis to the liver and stimulates pancreatic insulin secretion, providing a feedback loop that connects skeletal muscle metabolism with the liver and the pancreas during metabolic stress. PMID: 18468620
    37. These findings present new insights into the role of the glucocorticoid receptor and FOXO family of transcription factors in the transcriptional regulation of the MuRF1 gene PMID: 18612045
    38. Findings aid the future exploration of the cellular function and therapeutic potential of MuRF1. PMID: 18795805
    39. This study demenostrated that the muscle RING finger 1 protein, human is reduced in skeletal muscle of chronic spinal cord-injured patients. PMID: 19533653

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  • 亞細胞定位：
    Cytoplasm. Nucleus. Cytoplasm, myofibril, sarcomere, M line. Cytoplasm, myofibril, sarcomere, Z line.
  • 組織特異性：
    Muscle specific. Selectively expressed in heart and skeletal muscle. Also expressed in the iris.
  • 數據庫鏈接：

    HGNC: 16007

    OMIM: 606131

    KEGG: hsa:84676

    STRING: 9606.ENSP00000363390

    UniGene: Hs.279709



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