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Human Dihydrotestosterone,DHT ELISA Kit

  • 中文名稱：
    人雙氫睪酮(DHT)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E07878h
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人雙氫睪酮(DHT)酶聯(lián)免疫試劑盒（CSB-E07878h）為競(jìng)爭(zhēng)法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的DHT含量。DHT即雙氫睪酮，是睪酮經(jīng)5α-還原酶作用轉(zhuǎn)化而來(lái)。它與雄激素性脫發(fā)、前列腺增生等密切相關(guān)。研究機(jī)制主要聚焦于其與雄激素受體結(jié)合，影響毛囊、前列腺等組織細(xì)胞的生長(zhǎng)、分化，為相關(guān)疾病的藥物研發(fā)提供方向。試劑盒檢測(cè)范圍為10 pg/mL-2000 pg/mL，本產(chǎn)品可廣泛應(yīng)用于激素作用機(jī)制研究、內(nèi)分泌系統(tǒng)功能評(píng)估、前列腺相關(guān)疾病模型構(gòu)建及藥物干預(yù)效果分析等科研領(lǐng)域，為探索DHT在生理病理過(guò)程中的調(diào)控網(wǎng)絡(luò)提供高效工具，適用于細(xì)胞實(shí)驗(yàn)、動(dòng)物模型及臨床前研究的數(shù)據(jù)獲取。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 別名：
    N/A
  • 縮寫(xiě)：
    DHT
  • 種屬：
    Homo sapiens (Human)
  • 樣本類(lèi)型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    10 pg/mL-2000 pg/mL
  • 靈敏度：
    5 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Signal Transduction
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human DHT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 91  
    Range % 86-97  
    1:2 Average % 89  
    Range % 85-99  
    1:4 Average % 93  
    Range % 86-105  
    1:8 Average % 94  
    Range % 87-103  
  • 回收率：
    The recovery of human DHT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 94 89-99  
    EDTA plasma (n=4) 97 92-105  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average    
    2000 0.179 0.185 0.182    
    500 0.313 0.319 0.316    
    160 0.523 0.529 0.526    
    40 0.818 0.802 0.810    
    10 1.317 1.330 1.324    
    0 1.952 1.943 1.948    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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