Human Dehydroepiandrosterone sulfate,DHEA-S ELISA Kit

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產品詳情

產品描述：

人脫氫表雄酮硫酸酯(DHEA-S)酶聯免疫試劑盒（CSB-E05105h）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的DHEA-S含量。DHEA - S即硫酸脫氫表雄酮，是腎上腺分泌的一種類固醇激素。它在人體代謝、免疫等生理過程中發揮作用。研究機制上，其參與多種信號通路，與激素平衡、細胞增殖等密切相關，在衰老、代謝疾病等領域是研究熱點。試劑盒檢測范圍為20 ng/mL-4000 ng/mL，適用于基礎研究領域中對激素代謝機制、內分泌系統功能評估、藥物干預效果分析等科研項目，為探索腎上腺功能、衰老相關激素變化或代謝性疾病模型提供可靠的數據支持本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

DHEA-S
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

20 ng/mL-4000 ng/mL
靈敏度：

10 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Signal Transduction
測定原理：

quantitative
測定方法：

Competitive

精密度：

Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human DHEA-S in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	85-103
1:2	Average %	96
	Range %	89-105
1:4	Average %	94
	Range %	90-100
1:8	Average %	90
	Range %	83-110

回收率：

The recovery of human DHEA-S spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	86-103
EDTA plasma (n=4)	89	84-93

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

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