在线日韩日本国产亚洲丨少妇伦子伦情品无吗丨欧美性猛交xxxx免费看蜜桃丨精品人妻系列无码一区二区三区丨亚洲精品无码不卡在线播放

Your Good Partner in Biology Research

Human Cytochrome P450 3A4(CYP3A4) ELISA kit

  • 中文名稱:
    人細(xì)胞色素P450 3A4(CYP3A4)酶聯(lián)免疫試劑盒
  • 貨號:
    CSB-EL006439HU
  • 規(guī)格:
    96T/48T
  • 價(jià)格:
    ¥3600/¥2500
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
    人細(xì)胞色素P450 3A4(CYP3A4)酶聯(lián)免疫試劑盒(CSB-EL006439HU)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿、細(xì)胞裂解物樣本中的CYP3A4含量。CYP3A4 是細(xì)胞色素 P450 酶家族成員,在人體藥物代謝中起關(guān)鍵作用。它能氧化多種內(nèi)源性和外源性物質(zhì)。研究機(jī)制聚焦于其底物特異性、代謝途徑、與藥物相互作用等方面,了解這些有助于優(yōu)化藥物療效、降低不良反應(yīng)。試劑盒檢測范圍為23.5 pg/mL-1500 pg/mL,適用于藥物代謝機(jī)制研究、體外肝細(xì)胞模型評估、藥代動(dòng)力學(xué)分析及環(huán)境毒物對代謝酶調(diào)控作用等科研領(lǐng)域,為研究CYP3A4介導(dǎo)的代謝通路提供可靠工具。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名:
    CYP3A4; CYP3A3; Cytochrome P450 3A4; 1,4-cineole 2-exo-monooxygenase; 1,8-cineole 2-exo-monooxygenase; Albendazole monooxygenase; sulfoxide-forming; Albendazole sulfoxidase; CYPIIIA3; CYPIIIA4; Cholesterol 25-hydroxylase; Cytochrome P450 3A3; Cytochrome P450 HLp; Cytochrome P450 NF-25; Cytochrome P450-PCN1; Nifedipine oxidase; Quinine 3-monooxygenase
  • 縮寫:
    CYP3A4
  • Uniprot No.:
  • 種屬:
    Homo sapiens (Human)
  • 樣本類型:
    serum, plasma, tissue homogenates, cell lysates
  • 檢測范圍:
    23.5 pg/mL-1500 pg/mL
  • 靈敏度:
    5.8 pg/mL
  • 反應(yīng)時(shí)間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領(lǐng)域:
    Metabolism
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of human CYP3A4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
     SampleSerum(n=4)
    1:1Average %90
    Range %85-99
    1:2Average %96
    Range %90-102
    1:4Average %97
    Range %89-105
    1:5Average %95
    Range %90-100
  • 回收率:
    The recovery of human CYP3A4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9894-103
    EDTA plasma (n=4)9895-100
  • 標(biāo)準(zhǔn)曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    15002.754 2.791 2.773 2.597
    7502.369 2.394 2.382 2.206
    3751.824 1.789 1.807 1.631
    187.51.057 1.082 1.070 0.894
    940.640 0.647 0.644 0.468
    470.435 0.465 0.450 0.274
    23.50.264 0.278 0.271 0.095
    00.175 0.176 0.176  
  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

產(chǎn)品評價(jià)

靶點(diǎn)詳情

  • 功能:
    A cytochrome P450 monooxygenase involved in the metabolism of sterols, steroid hormones, retinoids and fatty acids. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase). Catalyzes the hydroxylation of carbon-hydrogen bonds. Exhibits high catalytic activity for the formation of hydroxyestrogens from estrone (E1) and 17beta-estradiol (E2), namely 2-hydroxy E1 and E2, as well as D-ring hydroxylated E1 and E2 at the C-16 position. Plays a role in the metabolism of androgens, particularly in oxidative deactivation of testosterone. Metabolizes testosterone to less biologically active 2beta- and 6beta-hydroxytestosterones. Contributes to the formation of hydroxycholesterols (oxysterols), particularly A-ring hydroxylated cholesterol at the C-4beta position, and side chain hydroxylated cholesterol at the C-25 position, likely contributing to cholesterol degradation and bile acid biosynthesis. Catalyzes bisallylic hydroxylation of polyunsaturated fatty acids (PUFA). Catalyzes the epoxidation of double bonds of PUFA with a preference for the last double bond. Metabolizes endocannabinoid arachidonoylethanolamide (anandamide) to 8,9-, 11,12-, and 14,15-epoxyeicosatrienoic acid ethanolamides (EpETrE-EAs), potentially modulating endocannabinoid system signaling. Plays a role in the metabolism of retinoids. Displays high catalytic activity for oxidation of all-trans-retinol to all-trans-retinal, a rate-limiting step for the biosynthesis of all-trans-retinoic acid (atRA). Further metabolizes atRA toward 4-hydroxyretinoate and may play a role in hepatic atRA clearance. Responsible for oxidative metabolism of xenobiotics. Acts as a 2-exo-monooxygenase for plant lipid 1,8-cineole (eucalyptol). Metabolizes the majority of the administered drugs. Catalyzes sulfoxidation of the anthelmintics albendazole and fenbendazole. Hydroxylates antimalarial drug quinine. Acts as a 1,4-cineole 2-exo-monooxygenase. Also involved in vitamin D catabolism and calcium homeostasis. Catalyzes the inactivation of the active hormone calcitriol (1-alpha,25-dihydroxyvitamin D(3)).
  • 基因功能參考文獻(xiàn):
    1. Experimental in silico and in vivo studies confirmed that the original Russian benzodiazepine phenazepam was the substrate of CYP3A4 isoenzyme. PMID: 29727298
    2. Apigenin showed reversible inhibition, acacetin, and chrysin showed combined irreversible and reversible inhibition while chrysin dimethylether, isorhamnetin, pinocembrin, and tangeretin showed pure irreversible inhibition. These results alert on possible flavonoiddrug interactions on the level of CYP3A4 PMID: 30301254
    3. This patient should be regarded as a CYP3A-poor metabolizer. PMID: 29469606
    4. spectroscopic studies clarified the heteroactivation of CYP3A4 caused by efavirenz with a proper affinity to the peripheral site PMID: 29191071
    5. The genetic polymorphisms of the multi-drug resistance-1 (MDR-1) and human cytochrome P450 3A (CYP3A4 and CYP3A5) genes were analyzed and compared between steroid sensitive, steroid resistant and control groups. PMID: 30143489
    6. this meta-analysis suggests that the CYP3A4*1B polymorphism might play a modest role in susceptibility to cancer, especially for leukemia. PMID: 30143500
    7. JNK is a novel mechanistic regulator of CYP3A4 induction by PXR. PMID: 29440179
    8. The other four metabolites were almost exclusively metabolized by CYP3A4. PMID: 29475834
    9. It observed an increase in the activity of CYP3A following but not during pregnancy when measured using the 4beta-hydroxycholesterol/cholesterol ratio. In addition, based on our results, it suggestes that the plasma 4beta-hydroxycholesterol/cholesterol ratio be used to measure CYP3A activity in pregnant women. PMID: 29759884
    10. These findings also suggest that EGF may be an important regulator of CYP3A4 expression in vivo. PMID: 29189189
    11. our results indicate that a significant level of MDR1 mRNA, but not CYP3A4 mRNA, is intrinsically present before chemotherapy in advanced STS tumors. PMID: 29689707
    12. A weak-to-moderate CYP3A4 induction by midostaurin. PMID: 29117990
    13. CYP3A4 *18B and GCK G-30A polymorphisms were related to new-onset diabetes after transplantation (p < 0.05). The lower concentration/dose or fasting serum glucose was in CYP3A4 *1/*1 carriers than that in *18B/*18B carriers in all the renal transplant recipients (p < 0.05), respectively. PMID: 29546446
    14. Data suggest high similarity in mechanisms involved in regulation of expression of pig CYP3A29 and human CYP3A4 in hepatocytes. PMID: 30153482
    15. rs7668282 (UGT2B7, T>C) was more prevalent in sodium valproate (VPA)-resistant patients than drug-responsive patients. rs2242480 (CYP3A4, C>T) and rs10188577 (SCN1A, T>C) were more prevalent in drug-responsive patients compared to drug-resistant patients. In children with generalized seizures on VPA therapy, polymorphisms of UGT2B7, CYP3A4, and SCN1A genes were associated with seizure reduction. PMID: 29679912
    16. There were no significant differences in the bosutinib C0 between genotypes for ABCB1, ABCG2, and CYP3A4 polymorphisms. PMID: 29736778
    17. These results suggest that human and marmoset P450 3A4/90 and 4F12 in livers or small intestines played important roles in terfenadine t-butyl hydroxylation. Marmosets could be a model for humans during first pass extraction of terfenadine and related substrates. PMID: 28436281
    18. This first description of CYP3A4*20 null genotype in liver-transplanted patients, supports the relevance of CYP3A genotyping in tacrolimus therapy PMID: 29256966
    19. We demonstrate for the first time that the effect of ABCB1 diplotype on tacrolimus disposition is dependent on both CYP3A5 and CYP3A4 genotype. PMID: 27378609
    20. Some flavonoids exhibit selective inhibition toward CYP3A4 rather than other major cytochromes. Here, data suggest high risk of herb-drug interactions or food-drug interactions could be caused by flavonoids; vital structural elements of natural flavonoids could lead to interactions with clinical drugs normally eliminated via CYP3A4 metabolism. PMID: 29753067
    21. The mAb concomitantly shifted IL-1beta IC50 values towards CYP3A4 activity. PMID: 28260174
    22. CYP3A4 was identified as a novel tumor suppressor gene related to a poor prognosis in HCC. PMID: 29109094
    23. In CYP3A4, the most energetically favorable docking mode places testosterone in a position with the methyl groups directed toward the heme iron PMID: 28986474
    24. this study indicates that CYP3A activity is regained subsequent to kidney transplantation PMID: 28928137
    25. Younger age, POR*28 allele, and CYP3A5*3 allele were associated with higher cyclosporine dosing requirements and lower concentration/dose ratio. PMID: 29135906
    26. Single nucleotide polymorphism rs4646437 in CYP3A4 at 7q21.1 (p = .0325), associated with alpha-adrenergic receptor antagonist drug effect in Benign Prostatic Hyperplasia. PMID: 28787260
    27. CYP3A4*1B is associated with CsA C0/Dose ratio in renal transplant recipients which indicates patients with CYP3A4*1B allele require lower dose of CsA to reach target blood concentration compared with the CYP3A4*1 carriers.[meta-analysis] PMID: 29678659
    28. The MDR1/CYP3A4/OPRM1 gene polymorphisms influenced the fentanyl consumption and the physiological effects of intravenous analgesia in the Chinese women who received lower segment caesarean section surgeries. PMID: 29601950
    29. Molecular dynamics simulations reveal significantly different modes of interactions of DND and ARVL with the substrate binding pocket and with a peripheral allosteric site. Interactions of both substrates with residues F213 and F219 at the allosteric site play a critical role in the communication of conformational changes induced by effector binding to productive binding of the substrate at the catalytic site. PMID: 29200287
    30. The results of this study suggested that the Prospective assaying of CYP3A-status (CYP3A4 expression, CYP3A5 genotype) may better identify the Alzheimer patients with higher risk of inefficiency or adverse reactions and may facilitate the improvement of personalized clozapine therapy PMID: 28340122
    31. In oral contraceptive users, haplotype A and B in the CYP3A4 gene were associated with venous thrombosis risk, but not in non-users; however, the effect on Sex-hormone-binding-globulin levels was not directional with the risk. PMID: 28579309
    32. The combined CYP3A4 and CYP3A5 genotype of renal transplant recipients has a major influence on the Tacrolimus dose required to reach the target exposure. PMID: 28704257
    33. We solved the 2.7 A crystal structure of the CYP3A4-midazolam (MDZ) complex, where the drug is well defined and oriented suitably for hydroxylation of the C1 atom, the major site of metabolism. This binding mode requires H-bonding to Ser119 and a dramatic conformational switch in the F-G fragment, which transmits to the adjacent helices, resulting in a collapse of the active site cavity and MDZ immobilization. PMID: 28031486
    34. Our results provide information on CYP3A4 polymorphisms in Tibetan individuals which may help to optimize pharmacotherapy effectiveness by providing personalized medicine to this ethnic group. PMID: 28674221
    35. Among two SNPs in CYP3A4 and CYP3A5 and 12 SNPs in ABCB1 gene, an association with ovarian cancer risk was observed for ABCB1 rs2157926 SNP only. PMID: 29491071
    36. Low CYP3A4 Activity is associated with Crohn's Disease. PMID: 28301431
    37. Cryopreserved human hepatocytes can be stored stably for more than a decade with little or no change in CYP3A4/5 induction. PMID: 28411281
    38. Associations between SLCO1B1 521C and cholesterol response were not detected in African Americans (n = 333). Associations between CYP3A4*22 or CYP3A5*3 and cholesterol response were not detected in whites or African Americans. PMID: 28482130
    39. This kdeg value for CYP3A4 was in good agreement with recently reported values. PMID: 28289057
    40. Sorafenib was poorly tolerated, and anti-Kaposi sarcoma (KS)activity was modest. Strong CYP3A4 inhibitors may contribute to sorafenib toxicity, and ritonavir has previously been shown to be a CYP3A4 inhibitor. Alternate antiretroviral agents without predicted interactions should be used when possible for concurrent administration with sorafenib PMID: 28341759
    41. A total of 84.8% of our patients were found to express both the CYP3capital A, Cyrillic5*3*3 genotype and CYP3capital A, Cyrillic4*1*1/*1*1B. Further studies are necessary in order to show the influence of these genetic polymorphisms on tacrolimus blood concentrations. PMID: 29314799
    42. This study has identified 4 synonymous variants in the HCN4 gene and 3 SNPs in the CYP3A4 gene. None of the variants appear to have a major effect on the reduction of HR produced by ivabradine. PMID: 27439367
    43. Methylation status of cytosine in the CYP3A4 proximal promoter correlated with changes in developmental expression of mRNA. PMID: 26772622
    44. Occupancy by modified histones was consistent with chromatin structural changes contributing to the mechanisms regulating CYP3A4 ontogeny. PMID: 26921389
    45. Hydroxyl metabolite (M3) of VX-509 which is formed via the aldehyde oxidase pathway is responsible for CYP3A4 inhibition. PMID: 27298338
    46. CYP3A5 genotype has minimal impact on the probability of quetiapine target attainment of the 1-hour concentrations but a significant impact on the 12-hour concentrations. PMID: 27137148
    47. CYP3A4 expression and N-acetyl transferase 2 acetylator phenotype can better identify the patients with higher risk of adverse reactions and can facilitate the improvement of personalized clonazepam therapy and withdrawal regimen. PMID: 27639091
    48. The dose-dependent FaFg of selective and dual CYP3A and/or P-gp substrates was well predicted. PMID: 27538919
    49. The magnitude of drug-drug interactions (DDIs) for studies with rifampicin and seven CYP3A4 probe substrates. PMID: 27026679
    50. This finding equated to lower cytochrome P450 isoform 3A4 (CYP3A4) EC50 values in the HepatoPac system. PMID: 27655038

    顯示更多

    收起更多

  • 亞細(xì)胞定位:
    Endoplasmic reticulum membrane; Single-pass membrane protein. Microsome membrane; Single-pass membrane protein.
  • 蛋白家族:
    Cytochrome P450 family
  • 組織特異性:
    Expressed in prostate and liver. According to some authors, it is not expressed in brain. According to others, weak levels of expression are measured in some brain locations. Also expressed in epithelium of the small intestine and large intestine, bile du
  • 數(shù)據(jù)庫鏈接:

    HGNC: 2637

    OMIM: 124010

    KEGG: hsa:1576

    STRING: 9606.ENSP00000337915

    UniGene: Hs.728751



主站蜘蛛池模板: 亚洲中文字幕无码中文字| 猫咪免费人成网站在线观看| 伊人婷婷六月狠狠狠去| 国产精品亚韩精品无码a在线| 一区二区视频| 亚欧成a人无码精品va片| 最新69国产成人精品视频| 99久久精品国产一区二区| 99国精品午夜福利视频不卡| 特黄特色大片免费播放| 久久中文字幕人妻丝袜| 国产ktv交换配乱婬视频| 国产精品久久毛片| 国产成人久久精品流白浆| 白丝美女被狂躁免费视频网站| 午夜性色福利在线视频福利 | 国产成人午夜福利在线观看视频| 天堂va欧美ⅴa亚洲va| 国产欧美va天堂在线观看视频下载 | 国产啪视频1000部免费| 欧美无遮挡很黄裸交视频| 国产肥白大熟妇bbbb| 天天爽天天摸天天碰| 国产放荡对白视频在线观看| 日日碰狠狠丁香久燥| 996久久国产精品线观看| 侵犯强奷高清无码| 亚洲欧洲国产成人综合在线观看| av在线观看地址| 亚洲高清乱码午夜电影网| 日本高清二区视频久二区| 国内精品久久久久久久97牛牛 | 在线播放免费人成视频在线观看| 三级在线看中文字幕完整版| 久久天天躁狠狠躁夜夜夜| 国产麻传媒精品国产av| 国产福利一区二区三区在线观看| 国产一区丝袜在线播放| 亚洲a∨无码国产精品久久网| 无码人妻av一区二区三区蜜臀| 亚洲视频日本有码中文|