在线日韩日本国产亚洲丨少妇伦子伦情品无吗丨欧美性猛交xxxx免费看蜜桃丨精品人妻系列无码一区二区三区丨亚洲精品无码不卡在线播放

Your Good Partner in Biology Research

Human Cytochrome-C,Cyt-C ELISA Kit

  • 中文名稱:
    人細胞色素C(Cyt-C)酶聯(lián)免疫試劑盒
  • 貨號:
    CSB-E08530h
  • 規(guī)格:
    96T/48T
  • 價格:
    ¥3200/¥2500
  • 其他:

產品詳情

  • 產品描述:
    人細胞色素C(Cyt-C)酶聯(lián)免疫試劑盒(CSB-E08530h)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、細胞裂解物樣本中的CYCS含量。CYCS(細胞色素c)是一種位于線粒體內膜上的電子傳遞蛋白,參與細胞呼吸鏈和細胞凋亡過程。其功能包括在線粒體電子傳遞鏈中傳遞電子,以及在細胞凋亡時從線粒體釋放到細胞質中,激活caspase-9并引發(fā)凋亡。研究CYCS有助于理解細胞能量代謝和程序性死亡調控。試劑盒檢測范圍為7.8 ng/mL-500 ng/mL,該產品主要應用于細胞凋亡機制研究、藥物誘導的線粒體功能評估、代謝性疾病模型建立等科研領域,例如在體外培養(yǎng)細胞模型中監(jiān)測凋亡進程,或通過檢測血清/血漿樣本探索疾病相關生物標志物。本品僅用于科研,不用于臨床診斷,產品具體參數(shù)及操作步驟詳見產品說明書。
  • 別名:
    CYC ELISA Kit; CYC_HUMAN ELISA Kit; CYCS ELISA Kit; Cytochrome c ELISA Kit; Cytochrome c somatic ELISA Kit; HCS ELISA Kit; THC4 ELISA Kit
  • 縮寫:
  • Uniprot No.:
  • 種屬:
    Homo sapiens (Human)
  • 樣本類型:
    serum, plasma, cell lysates
  • 檢測范圍:
    7.8 ng/mL-500 ng/mL
  • 靈敏度:
    1.95 ng/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Cell Biology
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of human Cyt-C in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
     SampleSerum(n=4)
    1:1Average %95
    Range %82-105
    1:2Average %92
    Range %85-100
    1:4Average %98
    Range %92-107
    1:8Average %90
    Range %83-98
  • 回收率:
    The recovery of human Cyt-C spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9489-99
    EDTA plasma (n=4)9588-104
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    5002.337 2.404 2.371 2.222
    2501.692 1.659 1.676 1.527
    1251.106 1.138 1.122 0.973
    62.50.675 0.662 0.669 0.520
    31.20.436 0.428 0.432 0.283
    15.60.257 0.273 0.265 0.116
    7.80.206 0.215 0.211 0.062
    00.153 0.145 0.149  
  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

引用文獻

產品評價

靶點詳情

  • 功能:
    Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain.; Plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Binding of cytochrome c to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases.
  • 基因功能參考文獻:
    1. Cytochrome c was upregulated in the primary Sjogren's syndrome patients, indicating the potential role of cytochrome c in the pathogenesis and development of primary Sjogren's syndrome. PMID: 29257225
    2. The caspase-8/Bid/cytochrome c axis links signals from death receptors to mitochondrial reactive oxygen species production. PMID: 28888620
    3. This work reveals a direct conformational link between the 40-57 Omega-loop of cytochrome c in which residue 41 resides and the dynamical properties of the axial ligand to the heme iron. PMID: 27461282
    4. The naturally occurring Y48H variant of cytochrome c in its oxidized heme state is more peroxidatic than either the Wild Type protein or the G41S variant that is also implicated in thrombocytopenia. PMID: 29083920
    5. ROCK activation phosphorylated Rac1b at Ser71 and increased reactive oxygen species (ROS) levels by facilitating the interaction between Rac1b and cytochrome c. Conversely, ROCK inactivation abolished their interaction, concomitant with ROS reduction. PMID: 28317242
    6. Data suggest that although HCCS mediates heme attachment to N-terminal cysteine in heme-attachment site (CXXXH) of cytochrome C variants, up to 50% of cytochrome C produced is modified in an oxygen-dependent manner, resulting in a mixed population of cytochrome c. [HCCS = holocytochrome c synthase] PMID: 28617588
    7. Data suggest that the stronger effect of K72A mutation on the peroxidase activity of human versus yeast cytochrome c results from relief of steric interactions between side chains at positions 72 and 81 (Ile in human vs Ala in yeast), which suppresses the dynamics of omega-loop D necessary for the intrinsic peroxidase activity of cytochrome c. PMID: 28598148
    8. These findings establish a framework for understanding the molecular basis of cytochrome c-mediated blocking of SET/TAF-Ibeta. PMID: 26216969
    9. Monitoring of serum cytochrome c might also serve as a sensitive apoptotic marker in vivo reflecting chemotherapy-induced cell death burden in patients with non-small cell lung cancer. PMID: 25578497
    10. G-Rh2 causes rapid and dramatic translocation of both Bak and Bax, which subsequently triggers mitochondrial cytochrome c release and consequent caspase activation. PMID: 23443079
    11. The mitochondrial metalloprotease OMA1 was activated in a Bax- and Bak-dependent fashion. PMID: 25275009
    12. In vitro ultrastructural changes of MCF-7 for metastasise bone cancer and induction of apoptosis via mitochondrial cytochrome C released by CaCO3/Dox nanocrystals PMID: 25028650
    13. a mechanism of multiple radical formations in the cytochrome c-phospholipid complexes under H2O2 treatment, consistent with the stabilization of the radical in the G41S mutant, which elicits a greater peroxidase activity from cytochrome c PMID: 24099549
    14. proposed that mutation of residue 41, and interaction with cardiolipin, increase peroxidase activity by altering the 40-57 Omega loop and its hydrogen bond network with the propionate of haem ring A; these changes enhance access of hydrogen peroxide and substrate to the haem PMID: 24329121
    15. Data indicate a novel missense mutation (Y48H) of the cytochrome c (CYCS) gene responsible for thrombocytopenia. PMID: 24326104
    16. results suggest the impact of residue 41 on the conformation of cytochrome c influences its ability to act in both of its physiological roles, electron transport and caspase activation PMID: 23334161
    17. structural characterization of cytochrome c in micelle PMID: 23070294
    18. Data indicate that the formation of cytochrome c-Apaf-1 apoptosome and the presence of Smac are absolutely required for PSAP-induced apoptosis. PMID: 23207240
    19. Spectroscopic analyses of HCCS alone and complexes of HCCS with site-directed variants of cytochrome c revealed the fundamental steps of heme attachment and maturation. PMID: 23150584
    20. The levels of cellular apoptosis-associated proteins such as Smac/DIABLO, Cyto C, and the activated fragment of caspase-3 increased in pancreatic cancer cells, but the expression of XIAP was significantly decreased after 24 h treatment with the combination of TRAIL and gemcitabine. PMID: 22320973
    21. CCN1 promotes the activation of p53 and p38 MAPK, which mediate enhanced cytochrome c release to amplify the cytotoxicity of TNFalpha. PMID: 22363611
    22. Translocation of ARTS initiates a first wave of caspase activation leading to the subsequent release of additional mitochondrial factors, including cytochrome C and SMAC/Diablo. PMID: 21869827
    23. Tyrosine phosphorylation turns alkaline transition into a biologically relevant process and makes human cytochrome c behave as an anti-apoptotic switch. PMID: 21706253
    24. mitochondrial import and direct electron transfer from cytochrome c to Rac1 modulates mitochondrial H(2)O(2) production in alveolar macrophages pulmonary fibrosis. PMID: 22157762
    25. Dynamic changes in cytochrome c distribution at the Raman band of 750 cm(-1) were observed after adding an apoptosis inducer to the cells. PMID: 22184220
    26. Specific nitration of tyrosines 46 and 48 makes cytochrome c assemble a non-functional apoptosome. PMID: 22192356
    27. Studies indicate that the CYCS mutation in TP Cargeegis a glycine 41 replacement by serine, which yields a cytochrome C variant with enhanced apoptotic pathway activity in vitro. PMID: 22102269
    28. Data show that G-Rh2 and Bet A cooperated to induce Bax traslocation to mitochondria and cytochrome c release, and enhanced cleavage of caspase-8 and Bid. PMID: 21751259
    29. Cerebrospinal fluid Bcl-2 and cytochrome C levels are elevated in adults after severe traumatic brain injury. PMID: 21448217
    30. Resveratrol induces p53-independent, X-linked inhibitor of apoptosis protein (XIAP)-mediated Bax protein oligomerization on mitochondria to initiate cytochrome c release and caspase activation. PMID: 21712378
    31. heme electronic structure change may ultimately be responsible for the enhanced proapoptotic activity of G41S mutated human cyt c PMID: 21192676
    32. it is the specific nitration of solvent-exposed Tyr74 which enhances the peroxidase activity and blocks the ability of cytochrome c to activate caspase-9, thereby preventing the apoptosis signaling pathway PMID: 20227384
    33. Data show that sorafenib initiated lethal apoptotic process through the release of cytochrome c and caspase 3/7 activation. PMID: 19770576
    34. Serum LRG when bound to extracellular Cyt c that is released from apoptotic cells acts as a survival factor for lymphocytes and possibly other cells that are susceptible to the toxic effect of extracellular Cyt c. PMID: 19851871
    35. NOA36/ZNF330 is translocated from the mitochondria to the cytoplasm when apoptosis is induced and that it contributes to cytochrome c release. PMID: 19895853
    36. Membrane-associated XIAP induces mitochondrial outer membrane permeabilization leading to cytochrome c and Smac release, which is dependent on Bax and Bak. PMID: 19875445
    37. galectin-3 is enriched in the mitochondria and prevents mitochondrial damage and cytochrome c release PMID: 11839755
    38. non-rare allelic variants of the Cyt c protein are absent in the populations analyzed in this study PMID: 16934433
    39. Mutation of human cytochrome c enhances the intrinsic apoptotic pathway and causes thrombocytopenia PMID: 18345000
    40. MICS1 individually functions in mitochondrial morphology and cytochrome c release. PMID: 18417609
    41. Serum cyto-c is a potent tumor marker as a predictor for malignant potential in several different types of cancer. PMID: 18825408
    42. Both neurons and cancer cells strictly inhibit cytochrome c-mediated apoptosis by a mechanism dependent on glucose metabolism. PMID: 19029908
    43. In 77 Italian patients with inherited thrombocytopenia and clinical and laboratory features similar to those of patients with the CYCS missense (Gly41Ser) mutation, no alterations of the open reading frame were identified. PMID: 19172527
    44. there was no evidence of somatic mutations of CYTOCHROME C in the cancers PMID: 19404857
    45. No difference in the serum level of cytochrome c was seen among the the groups of patients with type 2 diabetes, controls or in subjects with IGT. PMID: 19640329

    顯示更多

    收起更多

  • 相關疾病:
    Thrombocytopenia 4 (THC4)
  • 亞細胞定位:
    Mitochondrion intermembrane space. Note=Loosely associated with the inner membrane.
  • 蛋白家族:
    Cytochrome c family
  • 數(shù)據(jù)庫鏈接:

    HGNC: 19986

    OMIM: 123970

    KEGG: hsa:54205

    STRING: 9606.ENSP00000307786

    UniGene: Hs.437060



主站蜘蛛池模板: 久久久久久久极品内射| 亚洲女同一区二区| 日韩av无码精品人妻系列| 六月丁香婷婷色狠狠久久| 亚洲一区二区三区波多野结衣 | 国产精品国产高清国产av| 狠狠色噜噜狠狠狠狠色综合久| 日本十八禁黄无遮禁视频免费| 国产亚洲精品第一综合另类| 色狠狠色狠狠综合天天| 欧美老肥妇做爰bbww| 亚洲色欲天天天堂色欲网| 国产制服丝袜欧美在线观看| 亚洲高清最新av网站| 大地资源中文第3页| 国产无遮挡色视频免费观看性色| 人妻无码中文专区久久五月婷| 国产xxx69麻豆国语对白| 不卡无码av一区二区三区| 奇米777四色成人影视| 内射爽无广熟女亚洲| 国产欧美综合一区二区三区| 亚洲美女国产精品久久久久久久久| 欧美亚洲日本国产其他| 欧亚乱熟女一区二区在线| 狠狠亚洲婷婷综合色香五月排名| 午夜伦4480yy私人影院久久| 国产末成年女av片一区二区| 国产精品igao视频| 青青国产揄拍视频| 国产精品卡一卡2卡3卡4| 99热这里只有精品免费播放| 国产亚洲精品aaaaaaa片| 99re久久热在这里精品| 无码办公室丝袜ol中文字幕| 高清无码视频直接看| 免费国产成人高清在线视频| 亚洲色精品三区二区一区| 97精品一区二区视频在线观看| 秋霞最新高清无码鲁丝片| 中文无码日韩欧免费视频 |