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  4. Human Cross-linked Carboxy-terminal telopeptide of type Ⅰ collagen, CTX-I ELISA Kit

Human Cross-linked Carboxy-terminal telopeptide of type Ⅰ collagen, CTX-I ELISA Kit

  • 中文名稱：
    人Ⅰ型膠原交聯(lián)羧基末端肽(CTX-I)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E11224h
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3200/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人Ⅰ型膠原交聯(lián)羧基末端肽(CTX-I)酶聯(lián)免疫試劑盒（CSB-E11224h）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的CTX-I含量。試劑盒檢測(cè)范圍為0.625 ng/mL-40 ng/mL，常用于骨質(zhì)疏松機(jī)制研究、骨關(guān)節(jié)疾病模型評(píng)估及抗骨吸收藥物開發(fā)等科研領(lǐng)域；適用于體外骨代謝標(biāo)志物檢測(cè)、細(xì)胞模型骨吸收活性分析及動(dòng)物實(shí)驗(yàn)樣本評(píng)估等非臨床研究場(chǎng)景，為骨生物學(xué)研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說(shuō)明書。
  • 別名：
    Cross-linked Carboxy-terminal telopeptide of type 1 collagen, CTX-1, Cross-linked Carboxy-terminal telopeptide of type I collagen, CTX-I
  • 縮寫：
    CTX-I
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    0.625 ng/mL-40 ng/mL
  • 靈敏度：
    0.156 ng/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Others
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human CTX-I in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 88  
    Range % 83-95  
    1:2 Average % 95  
    Range % 91-98  
    1:4 Average % 97  
    Range % 90-103  
    1:8 Average % 96  
    Range % 91-101  
  • 回收率：
    The recovery of human CTX-I spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 85 81-92  
    EDTA plasma (n=4) 96 93-106  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    40 2.995 2.960 2.978 2.838  
    20 2.557 2.570 2.564 2.424  
    10 1.666 1.765 1.716 1.576  
    5 0.839 0.886 0.863 0.723  
    2.5 0.491 0.524 0.508 0.368  
    1.25 0.263 0.251 0.257 0.117  
    0.625 0.201 0.212 0.207 0.067  
    0 0.138 0.142 0.140 　  
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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相關(guān)問(wèn)答

■ 常見問(wèn)題解答
Q:

Please provide the following information regarding Human Cross-linked Carboxy-terminal telopeptide of type I collagen, CTX-I ELISA Kit CSB-E11224h
a. Is the capture antibody a monoclonal or polyclonal?
b. What is the host of the capture antibody?
c. Is the detection antibody a monoclonal or polyclonal?
d. What is the host of the detection antibody?
e. B208. What’s is that standard? A recombinant protein or something else? If a recombinant protein, what is the host?
f. If recombinant protein, is it full length or partial?
g. What is the species of the standard– human or something else?

A:
Thanks for your inquiry.
CSB-E11224h
Capture antibody is mouse monoclonal antibody and detection antibody is goat polyclonal antibody.
Standard is synthetic peptide.
Pls let me know if you have any further questions. Thank you.


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