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  4. Human Complement component C8 alpha chain(C8A) ELISA kit

Human Complement component C8 alpha chain(C8A) ELISA kit

  • 中文名稱：
    人補體蛋白C8α(C8A)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-EL004194HU
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人補體蛋白C8α(C8A)酶聯(lián)免疫試劑盒（CSB-EL004194HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的C8A含量。人補體蛋白C8α（C8A）是補體系統(tǒng)膜攻擊復(fù)合物（MAC）的核心組分，參與細胞膜攻擊，在固有免疫和炎癥反應(yīng)中起關(guān)鍵作用。其研究機制涉及C8A與C9的結(jié)合及自聚合，形成靶細胞膜上的孔狀結(jié)構(gòu)，以介導(dǎo)細胞裂解。試劑盒檢測范圍為1.56 ng/mL-100 ng/mL，該產(chǎn)品可應(yīng)用于免疫系統(tǒng)相關(guān)的基礎(chǔ)研究，如探究補體激活通路調(diào)控機制、炎癥性疾病模型中的補體功能變化，或評估實驗條件下（如藥物處理、基因編輯）生物樣本中C8α的濃度波動。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    C8A ELISA Kit; CO8A_HUMAN ELISA Kit; Complement component 8 subunit alpha ELISA Kit; Complement component 8, alpha polypeptide ELISA Kit; Complement component C8 alpha chain ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    1.56 ng/mL-100 ng/mL
  • 靈敏度：
    0.39 ng/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human C8A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1000 Average % 85
    Range % 80-89
    1:2000 Average % 94
    Range % 90-98
    1:4000 Average % 96
    Range % 92-100
    1:8000 Average % 89
    Range % 84-93
  • 回收率：
    The recovery of human C8A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 98 94-102
    EDTA plasma (n=4) 104 100-109
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected
    100 2.563 2.472 2.518 2.373
    50 2.272 2.169 2.221 2.076
    25 1.729 1.684 1.707 1.562
    12.5 1.253 1.293 1.273 1.128
    6.25 0.785 0.801 0.793 0.648
    3.12 0.464 0.462 0.463 0.318
    1.56 0.316 0.320 0.318 0.173
    0 0.141 0.148 0.145 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 功能：
    Constituent of the membrane attack complex (MAC) that plays a key role in the innate and adaptive immune response by forming pores in the plasma membrane of target cells. C8A inserts into the target membrane, but does not form pores by itself.
  • 基因功能參考文獻：
    1. C8 alpha and C8 beta have correspondingly similar roles in MAC-mediated lysis of erythrocytes and bacterial killing. C8 gamma is not required for complement-mediated killing of Gram-negative bacteria. PMID: 12413696
    2. Results describe the crystal structure of the human C8 alpha MACPF domain disulfide-linked to C8 gamma (alphaMACPF-gamma) at 2.15 A resolution. PMID: 18440555
    3. The binding specificity between C8 alpha and C8 beta subunits is determined by a cooperative interaction of the N-terminal thrombospondin type 1 module and the membrane attack complex/perforin domain. PMID: 12220191
    4. Both N-terminal modules in C8 alpha have a role in forming the principal binding site for C9, and binding may be dependent on a cooperative interaction between these modules and the C8 alpha membrane attack complex/perforin domain. PMID: 12463754
    5. result suggests that this segment of C8alpha and corresponding segments of the other MAC family members are independently folded domains PMID: 16618117
    6. One can predict that the indel segment of C8alpha assumes a conformation that allows for multiple points of contact with C8gamma. PMID: 16935577
    7. crystal structure of the MACPF domain of complement component C8alpha at 2.5 angstrom resolution was determined and it was shown that it is structurally homologous to the bacterial, pore-forming, cholesterol-dependent cytolysins PMID: 17872444
    8. C8gamma binds an indel peptide of C8alpha sequence and forms a non-covalent complex. PMID: 19048311

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  • 相關(guān)疾病：
    Complement component 8 deficiency, 1 (C8D1)
  • 亞細胞定位：
    Secreted. Cell membrane; Multi-pass membrane protein. Note=Secreted as soluble protein. Inserts into the cell membrane of target cells.
  • 蛋白家族：
    Complement C6/C7/C8/C9 family
  • 數(shù)據(jù)庫鏈接：

    HGNC: 1352

    OMIM: 120950

    KEGG: hsa:731

    STRING: 9606.ENSP00000354458

    UniGene: Hs.93210



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