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Human Complement C1q-like protein 3(C1QL3) ELISA kit

  • 中文名稱:
    人補體C1q樣蛋白3(C1QL3)酶聯免疫試劑盒
  • 貨號:
    CSB-EL003644HU
  • 規格:
    96T/48T
  • 價格:
    ¥3600/¥2500
  • 其他:

產品詳情

  • 產品描述:
    人補體C1q樣蛋白3(C1QL3)酶聯免疫試劑盒(CSB-EL003644HU)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的C1QL3含量。試劑盒檢測范圍為0.312 ng/mL-20 ng/mL,支持科研人員通過血清或血漿樣本探索C1QL3在生理病理過程中的動態變化,或利用組織勻漿分析其在特定器官中的表達特征,為代謝調控機制、神經免疫互作等基礎研究提供可靠工具本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 別名:
    C1q and tumor necrosis factor-related protein 13 ELISA Kit; C1q/TNF-related protein 13 ELISA Kit; C1ql ELISA Kit; C1QL3 ELISA Kit; C1QL3_HUMAN ELISA Kit; C1QTNF13 ELISA Kit; Complement C1q-like protein 3 ELISA Kit; Complement component 1; q subcomponent-like 3 ELISA Kit; CTRP13 ELISA Kit; K100 ELISA Kit
  • 縮寫:
    C1QL3
  • Uniprot No.:
  • 種屬:
    Homo sapiens (Human)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測范圍:
    0.312 ng/mL-20 ng/mL
  • 靈敏度:
    0.078 ng/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Others
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of human C1QL3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 105  
    Range % 100-110  
    1:2 Average % 99  
    Range % 95-107  
    1:4 Average % 89  
    Range % 85-93  
    1:8 Average % 84  
    Range % 80-88  
  • 回收率:
    The recovery of human C1QL3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 95 90-100  
    EDTA plasma (n=4) 90 85-95  
                 
                 
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    20 2.510 2.350 2.430 2.292  
    10 1.961 1.856 1.909 1.771  
    5 1.276 1.324 1.300 1.162  
    2.5 0.799 0.825 0.812 0.674  
    1.25 0.453 0.427 0.440 0.302  
    0.625 0.347 0.372 0.360 0.222  
    0.312 0.262 0.259 0.261 0.123  
    0 0.141 0.135 0.138    
  • 數據處理:
  • 貨期:
    3-5 working days

產品評價

靶點詳情

  • 功能:
    May regulate the number of excitatory synapses that are formed on hippocampus neurons. Has no effect on inhibitory synapses. Plays a role in glucose homeostasis. Via AMPK signaling pathway, stimulates glucose uptake in adipocytes, myotubes and hepatocytes and enhances insulin-stimulated glucose uptake. In a hepatoma cell line, reduces the expression of gluconeogenic enzymes G6PC1 and PCK1 and hence decreases de novo glucose production.
  • 基因功能參考文獻:
    1. CTRP13 is a novel adipokine that is down-regulated in the serum of type 2 diabetics. PMID: 28182339
    2. decreased levels of CTRP3 and especially CTRP13 were associated with increased risk of T2DM and CAD PMID: 28033351
    3. CTRP13 reduces glucose output in hepatocytes by inhibiting the mRNA expression of gluconeogenic enzymes, glucose-6-phosphatase and the cytosolic form of phosphoenolpyruvate carboxykinase. PMID: 21378161
  • 亞細胞定位:
    Secreted.
  • 組織特異性:
    Highly expressed in adipose tissue, with expression levels at least 2 orders of magnitude higher than in other tissues, including brain and kidney.
  • 數據庫鏈接:

    HGNC: 19359

    OMIM: 615227

    KEGG: hsa:389941

    UniGene: Hs.676792



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