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  4. Human Complement 7,C7 ELISA Kit

Human Complement 7,C7 ELISA Kit

  • 中文名稱：
    人補體7(C7)酶聯免疫試劑盒
  • 貨號：
    CSB-E11167h
  • 規格：
    96T/48T
  • 價格：
    ￥3200/￥2500
  • 其他：

產品詳情

  • 產品描述：
    人補體7(C7)酶聯免疫試劑盒（CSB-E11167h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的C7含量。試劑盒檢測范圍為15.6 ng/mL-1000 ng/mL，適用于科研場景中探索C7在感染性疾病（如細菌/病毒感染）、自身免疫疾病（如系統性紅斑狼瘡）或心血管疾病（如動脈粥樣硬化）中的調控機制，也可用于評估藥物干預或基因修飾對補體系統的影響。實驗支持多種生物樣本類型，為研究補體通路功能、炎癥反應及免疫相關疾病提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    C7 ELISA Kit; CO7_HUMAN ELISA Kit; complement component 7 ELISA Kit; Complement component C7 ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    15.6 ng/mL-1000 ng/mL
  • 靈敏度：
    3.9 ng/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Immunology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human C7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:100Average %91
    Range %87-95
    1:200Average %95
    Range %90-100
    1:400Average %97
    Range %94-101
    1:800Average %94
    Range %91-98
  • 回收率：
    The recovery of human C7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9087-94
    EDTA plasma (n=4)9993-105
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    10002.934 2.880 2.907 2.806
    5002.102 2.195 2.149 2.048
    2501.421 1.504 1.463 1.362
    1250.807 0.772 0.790 0.689
    62.50.443 0.459 0.451 0.350
    31.20.269 0.259 0.264 0.163
    15.60.171 0.167 0.169 0.068
    00.099 0.103 0.101
  • 數據處理：
  • 貨期：
    3-5 working days

產品評價

靶點詳情

  • 功能：
    Constituent of the membrane attack complex (MAC) that plays a key role in the innate and adaptive immune response by forming pores in the plasma membrane of target cells. C7 serves as a membrane anchor.
  • 基因功能參考文獻：
    1. this study shows that GG genotype of C7 provides protection against fibrosis severity while showing a higher risk for hepatocellular carcinoma in patients with hepatitis C PMID: 29966690
    2. Two case reports of C7 deficiency leading to recurrent meningitis and other bacterial infections are described. PMID: 28078901
    3. The complement C7 rs6876739 CC genotypes and mannan-binding lectin (MBL2) gene polymorphisms of liver donors were significantly associated with bacterial infection in recipients. PMID: 27063552
    4. Complement proteins C7 and CFH control the stemness of liver cancer cells via LSF-1 pathway. PMID: 26723877
    5. Borrelial CspA binds the human terminal complement components C7 and C9 and blocks assembly and membrane insertion of the terminal complement complex (TCC). PMID: 23943762
    6. Studies indicate that the deletion defect may be a more commonly distributed cause of C7 deficiency in Ireland. PMID: 22206826
    7. C7 isoelectric focusing variants can determine meningococcal killing in the early stage of infection when antibody-independent killing prevails. PMID: 19931914
    8. To determine transcriptional regulation of the human complement component C7, a 1 kb promoter fragment was cloned and the transcription start site was determined. C7 is expressed by the hepatoma-derived cell line Hep-3B, but not by Hep-G2. PMID: 12595902
    9. The interaction between the factor I domain of C7 and the C345C domain at the C terminus of the C5 alpha-chain plays an essential role in complement membrane attack complex formation and complement lytic activity. PMID: 15879120
    10. recurrence of fulminant meningococcal disease in a complement component C7-deficient patient PMID: 15889368
    11. membrane associated C7 acts as a trap for the late complement components to control excessive inflammation induced by SC5b-9 PMID: 19179470
    12. C7 is associated with multiple sclerosis pathogenesis. PMID: 19221116
    13. Data show that the two C7-FIMs pack closely together with an approximate 2-fold rotational symmetry that is rarely seen in module pairs and has not been observed in FD-containing proteins. PMID: 19419965

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  • 相關疾病：
    Complement component 7 deficiency (C7D)
  • 亞細胞定位：
    Secreted.
  • 蛋白家族：
    Complement C6/C7/C8/C9 family
  • 數據庫鏈接：

    HGNC: 1346

    OMIM: 217070

    KEGG: hsa:730

    STRING: 9606.ENSP00000322061

    UniGene: Hs.669878



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