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  4. Human Collectin-10(COLEC10) ELISA kit

Human Collectin-10(COLEC10) ELISA kit

  • 中文名稱：
    人膠原凝集素-10(COLEC10)酶聯免疫試劑盒
  • 貨號：
    CSB-EL005760HU
  • 規格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產品詳情

  • 產品描述：
    人膠原凝集素-10(COLEC10)酶聯免疫試劑盒（CSB-EL005760HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的COLEC10含量。COLEC10是一種C型凝集素家族成員，主要在肝星狀細胞中表達，參與肝纖維化的發病機制。其功能涉及免疫反應和炎癥過程，可能通過識別病原體相關分子模式（PAMP）和損傷相關分子模式（DAMP）來激活免疫細胞，增強宿主對感染的防御。試劑盒檢測范圍為23.44 pg/mL-1500 pg/mL，適用于細胞生物學、免疫學及疾病機制研究中COLEC10的表達動態分析，例如炎癥模型構建、病原體感染應答研究或腫瘤相關分子網絡探索。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    CL-34 ELISA Kit; CL-L1 ELISA Kit; CLL1 ELISA Kit; COL10_HUMAN ELISA Kit; Colec10 ELISA Kit; Collectin liver 1 ELISA Kit; Collectin liver protein 1 ELISA Kit; Collectin sub family member 10 (C-type lectin) ELISA Kit; Collectin sub family member 10 ELISA Kit; Collectin subfamily member 10 ELISA Kit; Collectin-10 ELISA Kit; Collectin-34 ELISA Kit; MGC118794 ELISA Kit; MGC118795 ELISA Kit; OTTHUMP00000228058 ELISA Kit
  • 縮寫：
    COLEC10
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    23.44 pg/mL-1500 pg/mL
  • 靈敏度：
    5.86 pg/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Immunology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human COLEC10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %87
    Range %81-92
    1:2Average %92
    Range %83-98
    1:4Average %97
    Range %93-101
    1:8Average %95
    Range %87-99
  • 回收率：
    The recovery of human COLEC10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9692-102
    EDTA plasma (n=4)8882-94
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    15002.880 2.964 2.922 2.814
    7502.541 2.427 2.484 2.376
    3751.832 1.734 1.783 1.675
    187.50.997 0.972 0.985 0.877
    93.750.533 0.554 0.544 0.436
    46.880.380 0.376 0.378 0.270
    23.440.254 0.258 0.256 0.148
    00.107 0.108 0.108
  • 數據處理：
  • 貨期：
    3-5 working days

引用文獻

產品評價

靶點詳情

  • 功能：
    Lectin that binds to various sugars: galactose > mannose = fucose > N-acetylglucosamine > N-acetylgalactosamine. Acts as a chemoattractant, probably involved in the regulation of cell migration.
  • 基因功能參考文獻：
    1. High CLL1 expression is associated with cardiovascular disease. PMID: 27341702
    2. Exome sequencing reveals mutations in collectin sub-family member 10 (C-type lectin) protein (COLEC10) as a cause of 3MC syndrome with main features include facial dysmorphism, craniosynostosis and cleft lip/palate. PMID: 28301481
    3. high serum CL-L1 concentration in critically ill children upon PICU admission is associated with an increased risk of infection and prolonged need of intensive care, and counteracts the protective effect of having a high MASP-3 concentration PMID: 27057739
    4. Data indicate differences in the plasma concentrations of collectin liver 1 and collectin kidney 1, M-ficolin and H-ficol in systemic lupus erythematosus (SLE) patients compared to a group of healthy controls. PMID: 26154564
    5. Carriers of COLEC10 Arg125Trp had increased CL-L1 serum levels. PMID: 25710878
    6. Most plasma CL-K1 was found in complex with CL-L1 in a ratio suggesting a heteromeric subunit of 1 CL-L1 & 2 CL-K1 polypeptides. PMID: 24174618

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  • 相關疾病：
    3MC syndrome 3 (3MC3)
  • 亞細胞定位：
    Secreted. Golgi apparatus. Cytoplasm.
  • 蛋白家族：
    COLEC10/COLEC11 family
  • 組織特異性：
    Highly expressed in liver, placenta and adrenal gland. Moderately expressed in small intestine, lung, stomach and prostate. Weakly expressed in trachea and spleen.
  • 數據庫鏈接：

    HGNC: 2220

    OMIM: 248340

    KEGG: hsa:10584

    STRING: 9606.ENSP00000332723

    UniGene: Hs.176615



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