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  4. Human Cocaine-and amphetamine-regulated transcript protein (Cartpt) ELISA kit

Human Cocaine-and amphetamine-regulated transcript protein (Cartpt) ELISA kit

  • 中文名稱：
    人可卡因-苯丙胺調節轉錄蛋白(Cartpt)酶聯免疫試劑盒
  • 貨號：
    CSB-EL004532HU
  • 規格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產品詳情

  • 產品描述：
    人可卡因-苯丙胺調節轉錄蛋白(Cartpt)酶聯免疫試劑盒（CSB-EL004532HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的Cartpt含量。人可卡因-苯丙胺調節轉錄蛋白（Cartpt）是一種下丘腦分泌的內源性神經肽，參與調節攝食、藥物成癮及激素分泌等生物過程。其表達受多種轉錄因子調控，如CREB和RFX5等，通過影響基因轉錄活性來調節其功能。試劑盒檢測范圍為15.6 pg/mL-1000 pg/mL，適用于體外檢測不同樣本來源的Cartpt表達差異，可應用于成癮行為機制、神經退行性疾病模型或精神疾病相關的基礎研究，為探索Cartpt在神經信號傳導及病理過程中的作用提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    CART ELISA Kit; CART prepropeptide ELISA Kit; CART(42-89) ELISA Kit; CART_HUMAN ELISA Kit; Cartpt ELISA Kit; Cocaine and amphetamine regulated transcript ELISA Kit
  • 縮寫：
    Cartpt
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    15.6 pg/mL-1000 pg/mL
  • 靈敏度：
    3.9 pg/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Others
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human Cartpt in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 91
    Range % 84-99
    1:2 Average % 88
    Range % 81-97
    1:4 Average % 95
    Range % 92-100
    1:8 Average % 94
    Range % 86-98
  • 回收率：
    The recovery of human Cartpt spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 95 89-98
    EDTA plasma (n=4) 94 90-98
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    1000 2.598 2.627 2.613 2.508
    500 2.248 2.296 2.272 2.167
    250 1.811 1.795 1.803 1.698
    125 1.186 1.201 1.194 1.089
    62.5 0.643 0.639 0.641 0.536
    31.2 0.368 0.359 0.364 0.259
    15.6 0.194 0.202 0.198 0.093
    0 0.103 0.106 0.105 　
  • 數據處理：
  • 貨期：
    3-5 working days

產品評價

靶點詳情

  • 功能：
    Satiety factor closely associated with the actions of leptin and neuropeptide Y; this anorectic peptide inhibits both normal and starvation-induced feeding and completely blocks the feeding response induced by neuropeptide Y and regulated by leptin in the hypothalamus. It promotes neuronal development and survival in vitro.
  • 基因功能參考文獻：
    1. Data suggest that the stress of intermittent hypoxia up-regulates expression of mRNA and protein for POMC and CART in neuronal cell lines; GATA2 and GATA3 appear to be involved in these stress mechanisms. (POMC = proopiomelanocortin; CART = cocaine- and amphetamine-regulated transcript protein; GATA2 = endothelial transcription factor GATA-2; GATA3 = T-cell-specific transcription factor GATA-3) PMID: 29275211
    2. Adenocarcinoma growth into the colon wall caused an increase in the relative frequency of CART-like immunoreactive (CART-LI) neurons in enteric plexuses located in the vicinity of the infiltrating neoplasm. PMID: 29385033
    3. In vitro neurochemical studies provide new insights into the interactions among CART peptide, dopamine and cocaine in the nucleus accumbens by showing a direct inhibitory effect of CART on dopamine both in normal conditions and after cocaine induction, as well as an effect of CART on the dopamine metabolites DOPAC, HVA and DOPET. PMID: 28802898
    4. Study conclude that CART is a regulator of glucose homeostasis and could play an important role in the pathophysiology of type 2 diabetes. Based on the ability of CART to increase insulin secretion and reduce glucagon secretion, CART-based agents could be a therapeutic modality in type 2 diabetes. PMID: 27338624
    5. polymorphisms in the gene may be useful in identifying women at risk for osteoporosis. PMID: 25871004
    6. In samples from patients undergoing IVF, there is a significant positive correlation between patient BMI, CART mRNA expression in granulosa cells, and CART peptide levels in follicular fluid. PMID: 26730935
    7. Colocalization experiments provided evidence for the presence of cocaine- and amphetamine-regulated transcript (CART) in KP-immunoreactive (IR) and perikarya and in KP-IR and NKB-IR axon varicosities. PMID: 25084101
    8. In schizophrenia patients on clozapine monotherapy, CART levels did not correlate with age, weight, BMI, abdominal, waist and hip circumferences, WHR, blood pressure, clozapine dose or treatment duration, body composition, or markers of insulin resistance PMID: 25219614
    9. Lower CART levels pose a risk for developing polycystic ovary syndrome. PMID: 24995854
    10. CART SNPs (rs10515115, rs3763153, rs3857384, rs11575893, rs16871471) were not associated with antipsychotic-induced weight gain in schizophrenia patients. PMID: 24564533
    11. CART is expressed in nerve fibers and co-localizes with other neuronal factors in the circular muscle layer of human descending colon PMID: 23277430
    12. Family-based association study failed to observe a statistically significant association for any of the genotyped SNPs in the CARTPT gene and attention-deficit hyperactivity disorder. PMID: 22436349
    13. Ischemia insult triggered by oxygen-glucose deprivation (OGD) enhances NRSF mRNA levels and then NRSF antagonizes the CREB signaling on CART activation, leading to augmented cell death. PMID: 23086924
    14. Serum CART can be detected in both diabetic and non-diabetic human pregnancy and may play an important role in body mass regulation in pregnancy. PMID: 22332848
    15. a detailed description of the CART distribution pattern within the human alimentary tract. PMID: 23034261
    16. The first report on the presence of CART-IR structures in the normal human pancreas. PMID: 22257587
    17. Expression of CART in small bowel carcinoid tumors is associated with worse survival. PMID: 22553341
    18. It was shown that cocaine- and amphetamine-regulated transcript protein (CART) increases the transcriptional activity of estrogen receptor alpha and that CART stimulates an autocrine/paracrine loop within tumor cells to amplify the CART signal. PMID: 22139072
    19. This study demonistrated thatsex difference in CART dynamic expressions in the midbrain of drug-free depressed suicide victims. PMID: 21803054
    20. No significant correlations are found between cerebrospinal fluid levels of orexin A and CART, fatigue, and hypersomnolence in multiple sclerosis patients. PMID: 21605873
    21. CART is an antioxidant peptide PMID: 22235287
    22. The CART c.517A-->G polymorphism was significantly associated with obesity susceptibility. PMID: 21921635
    23. CART is produced in the majority of neuroendocrine tumors, regardless of tumor origin. PMID: 21832817
    24. genetic variants in the CART gene may not play a major role in the vulnerability of schizophrenia subjects to concurrent alcohol use disorders and nicotine dependence. PMID: 20362026
    25. Our results supported the hypothesis that inherited variations of the CART gene could influence the development of obesity also in Italian children. PMID: 20151954
    26. genetic variants involved in weight gain, development of obesity PMID: 11791158
    27. Review. The genetics of CART and its role in reward, stress, and feeding behavior are reviewed. PMID: 12147208
    28. AvaII polymorphism (rs2239670) in intron 1 of the CART gene is associated with alcoholism in the Korean male population. PMID: 15234472
    29. Mutating codon 34 of human proCART alters the levels of CART peptides in transfected AtT20 cells. PMID: 15326462
    30. CART SNP -3608T>C may possibly contribute to the genetic risk for obesity in the Caucasian population PMID: 15823203
    31. CART has a role in the control of appetite [review] PMID: 16102267
    32. Cocaine- and amphetamine-regulated transcript peptides (55 to 102 & 62 to 102) are neurotransmitters with roles in a number of processes, including drug abuse by virtue of the fact that they are modulators of mesolimbic function [Review] PMID: 16146347
    33. Obesity observed in humans bearing the Leu34Phe mutation could be due to a putative deficiency in hypothalamic bioactive CART. PMID: 16210370
    34. analysis of CART binding sites on HepG2 cells and mouse hypothalamic dissociated cells PMID: 16309793
    35. CART signaling is the main molecular pathway accounting for the decrease in bone resorption leading to high bone mass in mice and humans deficient in Mc4r PMID: 16614075
    36. The anatomy of CART-containing neurons in the human infundibular nucleus raises the question as to whether this is asssociated with neuropeptide Y and agouti related protein. PMID: 17525122
    37. reviews the localization of CART mRNA expression in the human brain and its possible relevance to human cocaine abuse [review] PMID: 17631364
    38. This study do not support an association of the CARTPT gene with cocaine dependence. PMID: 18572320
    39. Data show that CSF CART levels are significantly reduced by 30% in Dementia with Lewy bodies patients compared to controls as well as to Alzheimer's disease patients using radioimmunoassay. PMID: 19356602
    40. Medium-sized CART-positive neurons are common in the medial division of the shell of nucleus accumbens in monkeys. CART may be involved in neuronal and behavioural changes that underlie addiction to psychomotor stimulants and feeding. PMID: 10235641
    41. The solution structure of the biologically active part of CART (residues 48-89) consists of turns and loops spanned by a compact framework composed of a few small stretches of antiparallel beta-sheet common to cystine knots. PMID: 11478874

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  • 亞細胞定位：
    Secreted.
  • 蛋白家族：
    CART family
  • 組織特異性：
    Hypothalamus. Found in neurons of the ventrolateral part of the arcuate nucleus, in the external zone of the median eminence, and also found in terminals in the periventricular part of the paraventricular nucleus.
  • 數據庫鏈接：

    HGNC: 24323

    OMIM: 602606

    KEGG: hsa:9607

    STRING: 9606.ENSP00000296777

    UniGene: Hs.1707



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