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Human Calcitonin,CT ELISA Kit

  • 中文名稱:
    人降鈣素(CT)酶聯免疫試劑盒
  • 貨號:
    CSB-E05131h
  • 規格:
    96T/48T
  • 價格:
    ¥3900/¥2500
  • 其他:

產品詳情

  • 產品描述:
    人降鈣素(CT)酶聯免疫試劑盒(CSB-E05131h)檢測血清、血漿、組織勻漿樣本中的CALCA含量。CALCA 是重要靶點。它在機體生理活動里有關鍵作用。研究機制上,CALCA 參與多種信號通路,其表達異常和疾病發生發展關聯緊密。科研聚焦于解析其作用途徑,期望通過干預 CALCA 開發治療疾病的新策略和藥物。試劑盒檢測范圍為4.688 pg/mL-300 pg/mL,該產品適用于基礎醫學研究中CT蛋白表達水平的定量檢測,如鈣代謝調控機制探索、骨骼疾病動物模型評估或甲狀腺功能相關病理研究,配套的血清/血漿前處理方案及組織勻漿制備指南可滿足不同實驗體系需求,為科研人員提供穩定可靠的檢測工具。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 別名:
    CALCA ELISA kit; CALC1Calcitonin [Cleaved into: Calcitonin; Katacalcin ELISA kit; Calcitonin carboxyl-terminal peptide ELISA kit; CCP ELISA kit; PDN-21)] ELISA kit
  • 縮寫:
  • Uniprot No.:
  • 種屬:
    Homo sapiens (Human)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測范圍:
    4.688 pg/mL-300 pg/mL
  • 靈敏度:
    1.172 pg/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Signal Transduction
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of human CT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 88  
    Range % 81-95  
    1:2 Average % 95  
    Range % 92-100  
    1:4 Average % 94  
    Range % 90-110  
    1:8 Average % 94  
    Range % 89-105  
  • 回收率:
    The recovery of human CT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 96 85-100  
    EDTA plasma (n=4) 92 88-99  
                 
                 
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    300 2.379 2.252 2.316 2.173  
    150 1.794 1.760 1.777 1.634  
    75 1.305 1.284 1.295 1.152  
    37.5 0.954 0.909 0.932 0.789  
    18.7 0.593 0.564 0.579 0.436  
    9.4 0.372 0.349 0.361 0.218  
    4.7 0.253 0.239 0.246 0.103  
    0 0.149 0.137 0.143    
  • 數據處理:
  • 貨期:
    3-5 working days

產品評價

靶點詳情

  • 功能:
    Calcitonin causes a rapid but short-lived drop in the level of calcium and phosphate in blood by promoting the incorporation of those ions in the bones.; Katacalcin is a potent plasma calcium-lowering peptide.
  • 亞細胞定位:
    Secreted.
  • 蛋白家族:
    Calcitonin family
  • 數據庫鏈接:

    HGNC: 1437

    OMIM: 114130

    KEGG: hsa:796

    STRING: 9606.ENSP00000331746

    UniGene: Hs.37058



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