在线日韩日本国产亚洲丨少妇伦子伦情品无吗丨欧美性猛交xxxx免费看蜜桃丨精品人妻系列无码一区二区三区丨亚洲精品无码不卡在线播放

Your Good Partner in Biology Research

  1. 首頁
  2. 試劑盒
  3. 酶聯免疫試劑盒
  4. Human Bromodomain testis-specific protein(BRDT) ELISA kit

Human Bromodomain testis-specific protein(BRDT) ELISA kit

  • 中文名稱：
    人溴結構域睪丸特異蛋白(BRDT)酶聯免疫試劑盒
  • 貨號：
    CSB-EL002807HU
  • 規格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產品詳情

  • 產品描述：
    人溴結構域睪丸特異蛋白(BRDT)酶聯免疫試劑盒（CSB-EL002807HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細胞裂解物樣本中的BRDT含量。BRDT是一種溴結構域蛋白。它與染色質的動態變化、基因表達調控密切相關。在研究機制上，BRDT可通過識別乙酰化組蛋白，調控精子發生等生理過程。目前，針對BRDT的研究旨在開發相關靶向藥物，用于癌癥、生殖疾病等的治療。試劑盒檢測范圍為62.5 pg/mL-4000 pg/mL，該產品適用于探索BRDT在睪丸發育、精子形成異常或生殖相關疾病模型中的表達變化，也可用于評估藥物或環境因素對生殖細胞表觀遺傳調控的影響，為生殖醫學基礎研究、雄性生育力評估及生殖毒性機制研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    BRDT ELISA Kit; Bromodomain testis-specific protein ELISA Kit; Cancer/testis antigen 9 ELISA Kit; CT9 ELISA Kit; RING3-like protein ELISA Kit
  • 縮寫：
    BRDT
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates, cell lysates
  • 檢測范圍：
    62.5 pg/mL-4000 pg/mL
  • 靈敏度：
    15.6 pg/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Epigenetics and Nuclear Signaling
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human BRDT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 92
    Range % 88-96
    1:2 Average % 87
    Range % 82-91
    1:4 Average % 105
    Range % 101-113
    1:8 Average % 97
    Range % 92-101
  • 回收率：
    The recovery of human BRDT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 95 90-100
    EDTA plasma (n=4) 84 80-88
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    4000 2.751 2.593 2.672 2.541
    2000 1.875 1.758 1.817 1.686
    1000 1.040 1.070 1.055 0.924
    500 0.592 0.611 0.602 0.471
    250 0.364 0.349 0.357 0.226
    125 0.239 0.227 0.233 0.102
    62.5 0.158 0.165 0.162 0.031
    0 0.127 0.134 0.131 　
  • 數據處理：
  • 貨期：
    3-5 working days

產品評價

靶點詳情

  • 功能：
    Testis-specific chromatin protein that specifically binds histone H4 acetylated at 'Lys-5' and 'Lys-8' (H4K5ac and H4K8ac, respectively) and plays a key role in spermatogenesis. Required in late pachytene spermatocytes: plays a role in meiotic and post-meiotic cells by binding to acetylated histones at the promoter of specific meiotic and post-meiotic genes, facilitating their activation at the appropriate time. In the post-meiotic phase of spermatogenesis, binds to hyperacetylated histones and participates in their general removal from DNA. Also recognizes and binds a subset of butyrylated histones: able to bind histone H4 butyrylated at 'Lys-8' (H4K8ac), while it is not able to bind H4 butyrylated at 'Lys-5' (H4K5ac). Also acts as a component of the splicing machinery in pachytene spermatocytes and round spermatids and participates in 3'-UTR truncation of specific mRNAs in post-meiotic spermatids. Required for chromocenter organization, a structure comprised of peri-centromeric heterochromatin.
  • 基因功能參考文獻：
    1. FBP1 modulates the sensitivity of pancreatic cancer cells to BET inhibitors by decreasing the expression of c-Myc. These findings highlight FBP1 could be used as a therapeutic niche for patient-tailored therapies PMID: 30201002
    2. The bromodomain and extraterminal domain (BET) family consists of BRDT, BRD2, BRD3, and BRD4, each containing 2 bromodomains located N-terminal to extraterminal domain, which is located near C-terminus. Data suggest that 10 distinct acylations participate in binding of BET family proteins to histone 4 (oligopeptide fragments used here); C-terminal bromodomains do not cooperatively bind multiple acylation sites. PMID: 28945351
    3. The affected patient carries a homozygous bromodomain, testis-specific protein (BRDT) mutation, while the patient's father, mother and elder brother all carry heterozygous BRDT mutations. PMID: 28199965
    4. considered potential associations of 14 single nucleotide polymorphisms (SNPs) in RNF8 and BRDT genes in Chinese patients with non-obstructive azoospermia PMID: 25374327
    5. assessment of rs3088232 frequency in large group of non-obstructive azoospermia men and fertile controls demonstrated no significant difference between them; conclude that testicular impairments observed were not a consequence of BRDT gene mutation PMID: 24865796
    6. In human, BRDT is the only BET gene expressed exclusively in testicular germ cells. PMID: 22035730
    7. Suggest that reduced histone methylation in the promoter of BRDT may be associated with increased transcript levels in subfertile patients. PMID: 20538714
    8. BRDT-NY gene is an alternatively spliced variant that may have an important role in the process of spermatogenesis and may be correlated with male infertility PMID: 15647849

    顯示更多

    收起更多

  • 相關疾病：
    Spermatogenic failure 21 (SPGF21)
  • 亞細胞定位：
    Nucleus.
  • 組織特異性：
    Testis-specific. A 3-fold higher expression is seen in adult testis than in embryo testis. Expression seems to be correlated with histone H4 hyperacetylation during the haploid phase of spermatogenesis (spermiogenesis). No expression, or very low expressi
  • 數據庫鏈接：

    HGNC: 1105

    OMIM: 602144

    KEGG: hsa:676

    STRING: 9606.ENSP00000354568

    UniGene: Hs.482520



主站蜘蛛池模板： 亚洲日韩片无码中文字幕| 久久天天躁狠狠躁夜夜网站| 久久久久久国产精品免费免费男同 | 中文国产成人精品久久久| 天天爽夜夜爽人人爽免费| 粉嫩av久久一区二区三区| 日日躁夜夜躁狠狠躁| 亚洲精品无码国产| 人人妻人人爽人人爽| 日韩欧美猛交xxxxx无码| 日韩精品久久久肉伦网站| 亚洲精品无码国产| 亚洲一区在线观看尤物| 强行糟蹋人妻hd中文字| 亚洲国产成人久久综合下载| 少妇高潮无套内谢麻豆传| 国产精品毛多多水多| 国产精品9999久久久久仙踪林| 亚洲乱码日产精品bd在线看| 国产成人年无码av片在线观看| 免费裸体无遮挡黄网站免费看| 久久人人爽人人爽人人片ⅴ| 夜夜欢性恔免费视频| 亚洲精品美女久久777777| 国产极品视觉盛宴| 精品性高朝久久久久久久| 人妻无码少妇一区二区| 天堂中文在线www天堂在线| 久久婷婷五月综合色丁香| 国产区又黄又硬高潮的视频| 69国产精品久久久久久人妻| 青青草97国产精品免费观看| 亚洲精品亚洲人成在线观看| 久久www成人片免费看| 国精产品一品二品国精在线观看| 国产成人免费爽爽爽视频| 无遮无挡爽爽免费视频| 亚洲午夜久久久精品影院| 成人片黄网站a毛片免费| 蜜臀av网站在线| 色偷偷色噜噜狠狠网站年轻人|