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  4. Human Beta-defensin 104(DEFB104A) ELISA kit

Human Beta-defensin 104(DEFB104A) ELISA kit

  • 中文名稱：
    人β防御素104(DEFB104A)酶聯免疫試劑盒
  • 貨號：
    CSB-EL006665HU
  • 規格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產品詳情

  • 產品描述：
    人β防御素104(DEFB104A)酶聯免疫試劑盒（CSB-EL006665HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養上清液、組織勻漿樣本中的DEFB104A含量。DEFB104A是一個靶點。其它可能在人體免疫等生理過程中有潛在作用。研究機制上，可能涉及參與免疫細胞的調控、抗菌防御等反應，但目前關于它的詳細背景及具體研究機制還需更多研究來進一步明確和完善。試劑盒檢測范圍為31.25 pg/mL-2000 pg/mL，尤其適用于感染性疾病模型、免疫調控機制研究以及抗菌肽相關的基礎科研，能夠為體外實驗模型中DEFB104A的動態變化提供可靠的定量數據支持，助力宿主防御機制及炎癥相關通路的探索。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    BD 4 ELISA Kit; BD-4 ELISA Kit; BD4 ELISA Kit; beta 104 ELISA Kit; beta 4 Defensin ELISA Kit; Beta defensin 4 ELISA Kit; Beta-defensin 104 ELISA Kit; Beta-defensin 4 ELISA Kit; D104A_HUMAN ELISA Kit; DEFB-4 ELISA Kit; DEFB104B ELISA Kit; Defensin ELISA Kit; Defensin beta 104 ELISA Kit; Defensin beta 4 precursor ELISA Kit; hBD 4 ELISA Kit; hBD-4 ELISA Kit; hBD4 ELISA Kit
  • 縮寫：
    DEFB104A
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 檢測范圍：
    31.25 pg/mL-2000 pg/mL
  • 靈敏度：
    7.8 pg/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Others
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human DEFB104A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %91
    Range %86-97
    1:2Average %92
    Range %87-100
    1:4Average %85
    Range %81-93
    1:8Average %92
    Range %86-98
  • 回收率：
    The recovery of human DEFB104A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 8984-96
    EDTA plasma (n=4)9285-96
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    20002.706 2.655 2.681 2.525
    10002.136 2.011 2.074 1.918
    5001.346 1.289 1.318 1.162
    2500.764 0.752 0.758 0.602
    1250.367 0.354 0.361 0.205
    62.50.279 0.287 0.283 0.127
    31.250.244 0.231 0.238 0.082
    00.162 0.150 0.156 　
  • 數據處理：
  • 貨期：
    3-5 working days

產品評價

靶點詳情

  • 功能：
    Has antimicrobial activity. Synergistic effects with lysozyme and DEFB103.
  • 基因功能參考文獻：
    1. Beta-defensin 104 displays antimicrobial activity against S. carnosus, E. coli, S. cerevisiae, and P. aeruginosa (strong). PMID: 11481241
    2. TFF3 activated the epithelial cells in culture to produce beta defensins 2 (hBD2) and beta defensins 4 PMID: 23198942
    3. Data suggest an activation of b-defensin-4 induction, in human knee meniscus by the osteoarthritis inflammatory process as a result of an endogenous antibiotic defense mechanism accompanied by an intrinsic effort of tissue remodeling. PMID: 21879330
    4. Our results suggest that copy number variation of DEFB4 may not contribute to the pathogenesis of Behcet's disease. PMID: 21385545
    5. Analysis of the DEFB4 promoter region shows remarkably high density of sequence variabilities. PMID: 20445567
    6. The variations in the genes encoding human beta-defensin-1 and -2 may be associated with the risk of severe acute pancreatitis. PMID: 20720450
    7. Data show that beta-defensin cluster (DEFB4, DEFB103 and DEFB104) varied between 2 and 9 copies per genome, and high copy numbers (>4) were underrepresented among patients, suggesting that increased copy numbers could protect from CD. PMID: 20483368
    8. We report here the application of small ubiquitin-related modifier (SUMO) fusion technology to the expression and purification of cationic antibacterial peptide hBD4. PMID: 20526896
    9. This study demonstrates, the involvement of NOD1 and DEFB4 in direct killing of Helicobacter pylori bacteria by epithelial cells and confirms the importance of NOD1 in host defence mechanisms against cagPAI(+)Helicobacter pylori infection. PMID: 20039881
    10. The mRNA level of the beta defensin 4 did not differ significantly between the two subgroups. PMID: 20128731
    11. elevated DEFB4 copy number is a risk factor for Crohn's disease PMID: 19809410
    12. There is an association of four common DEFB104 haplotypes with the risk of prostate cancer in two independent patient cohorts. PMID: 18515986

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  • 亞細胞定位：
    Secreted.
  • 蛋白家族：
    Beta-defensin family
  • 組織特異性：
    High expression in the testis. Gastric antrum exhibited relatively high levels. A lower expression is observed in uterus and neutrophils thyroid gland, lung, and kidney. No detectable expression in other tissues tested.
  • 數據庫鏈接：

    HGNC: 18115

    KEGG: hsa:107984148

    STRING: 9606.ENSP00000322191

    UniGene: Hs.559507



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