在线日韩日本国产亚洲丨少妇伦子伦情品无吗丨欧美性猛交xxxx免费看蜜桃丨精品人妻系列无码一区二区三区丨亚洲精品无码不卡在线播放

Your Good Partner in Biology Research

Human Beta-1,4-galactosyltransferase 1(B4GALT1) ELISA kit

  • 中文名稱:
    人β-1,4-半乳糖基轉移酶1(B4GALT1)酶聯免疫試劑盒
  • 貨號:
    CSB-EL002513HU
  • 規格:
    96T/48T
  • 價格:
    ¥3600/¥2500
  • 其他:

產品詳情

  • 產品描述:
    人β-1,4-半乳糖基轉移酶1(B4GALT1)酶聯免疫試劑盒(CSB-EL002513HU)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、細胞裂解物、組織勻漿樣本中的B4GALT1含量。B4GALT1是一種β-1,4-半乳糖基轉移酶,參與N-糖基化過程,與多種疾病相關。研究發現,B4GALT1在降低LDL-C和纖維蛋白原水平方面具有重要作用,同時,其在肺腺癌免疫逃逸中發揮關鍵作用,可能成為治療靶點。試劑盒檢測范圍為0.156 ng/mL-10 ng/mL,適用于基礎科研中探索B4GALT1在腫瘤微環境、代謝調控或細胞分化等領域的生物學功能,亦可配合細胞實驗或動物模型進行蛋白表達動態分析。為生命科學領域的機制研究提供可靠工具。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 別名:
    B4GALT1; GGTB2; Beta-1,4-galactosyltransferase 1; Beta-1,4-GalTase 1; Beta4Gal-T1; b4Gal-T1; Beta-N-acetylglucosaminyl-glycolipid beta-1,4-galactosyltransferase; Beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase; Lactose synthase A protein; N-acetyllactosamine synthase; Nal synthase; Neolactotriaosylceramide beta-1,4-galactosyltransferase; UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 1; UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 1
  • 縮寫:
    B4GALT1
  • Uniprot No.:
  • 種屬:
    Homo sapiens (Human)
  • 樣本類型:
    serum, plasma, cell lysates, tissue homogenates
  • 檢測范圍:
    0.156 ng/mL-10 ng/mL
  • 靈敏度:
    0.039 ng/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Signal Transduction
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of human B4GALT1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)
    1:1 Average % 96
    Range % 90-102
    1:2 Average % 89
    Range % 84-95
    1:4 Average % 91
    Range % 87-99
    1:8 Average % 92
    Range % 88-96
  • 回收率:
    The recovery of human B4GALT1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 86 80-90
    EDTA plasma (n=4) 100 95-108
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected
    10 2.201 2.153 2.177 2.022
    5 1.731 1.659 1.695 1.540
    2.5 1.291 1.264 1.278 1.123
    1.25 0.836 0.892 0.864 0.709
    0.625 0.647 0.670 0.659 0.504
    0.312 0.393 0.375 0.384 0.229
    0.156 0.264 0.271 0.268 0.113
    0 0.153 0.156 0.155  
  • 數據處理:
  • 貨期:
    3-5 working days

產品評價

靶點詳情

  • 功能:
    The Golgi complex form catalyzes the production of lactose in the lactating mammary gland and could also be responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids.; The cell surface form functions as a recognition molecule during a variety of cell to cell and cell to matrix interactions, as those occurring during development and egg fertilization, by binding to specific oligosaccharide ligands on opposing cells or in the extracellular matrix.
  • 基因功能參考文獻:
    1. Human beta-1,4-GalT1 is a constitutively expressed mesangial cell IgA receptor with an important role in both mesangial IgA clearance and the initial response to IgA deposition. PMID: 28750925
    2. Increased B4GALT1 expression is a potential independent adverse prognostic factor for overall survival in patients with non-metastatic clear cell renal cell carcinoma. PMID: 27092876
    3. demonstrated that ZFX is aberrantly expressed in multiple human leukemic cells and it modulates the growth and drug response of leukemic cells partially via B4GALT1, which suggests that ZFX is a new regulator of leukemic cells and warrants intensive investigations on this 'stemness' regulator in these deadly diseases PMID: 27797721
    4. High B4GALT1 expression is associated with aging. PMID: 26840264
    5. We suggest that the unique expression patterns for the B4GALT1 in normal and malignant tissues are controlled by a differential usage of 5'-B4GALT1 regulatory units along with a post-transcriptional regulation by the antisense RNA PMID: 26315939
    6. Human chorionic gonadotropin provides a mechanism to bridge embryo to endometrium through beta1,4-GalT. PMID: 26191157
    7. HS5 cells had significantly enhanced levels of bisecting N-glycans (catalyzed by MGAT3 whereas HS27a cells had enhanced levels of Galbeta1,4GlcNAc. PMID: 25936519
    8. plays a role in activation of T-lymphocytes and participates in intercellular contact formation PMID: 25223470
    9. B4GALT1 and B4GALT5, two members of B4GALT gene family, are involved in the development of multidrug resistance of human leukemia cells. PMID: 23744354
    10. the glycogene B4GALT1 represent a valuable candidate biomarker of invasive phenotype of colorectal cancer. PMID: 22927297
    11. RNAi-mediated knockdown of beta1,4GT1 increased the levels of EGFR dimerization and phosphorylation. These data suggest that cell surface beta1,4GT1 interacts with EGFR and inhibits EGFR activatio PMID: 23583406
    12. estrogen regulates the expression of B4GALT1 through the direct binding of ER-alpha to ERE; the expressed B4GALT1 plays a crucial role in the proliferation of MCF-7 cells through its activity as a membrane receptor. PMID: 22982306
    13. Osteopontin increases the expression of beta1, 4-Galactosyltransferase-I and promotes adhesion in human RL95-2 cells, the activity critical for embryo implantation. PMID: 22847114
    14. expression of beta1,4-GalT-I increased in the cartilage and synovial tissue of osteoarthritis (OA) patients compared with healthy controls; data suggest that beta1,4-GalT-I may play an important role in the inflammatory processes in cartilage and synovial tissue of patients with OA PMID: 21750942
    15. beta4Gal-T1 molecule has two different oligosaccharide binding regions for the binding of the extended oligosaccharide moiety of the acceptor substrate. PMID: 22740701
    16. TNF-alpha contributes to the up-regulation of beta1,4-GalT-I mRNA in human fibroblast-like synoviocytes. PMID: 20886274
    17. These results suggested that beta1,4-Galactosyltransferase-I may play an important role in the inflammatory processes in synovial tissue of patients with rheumatoid arthritis. PMID: 20490888
    18. B4GalTI might act as a key adhesion molecule participating in T cell-dendritic cell contacts. PMID: 20851383
    19. PKC signal transduction pathway participates in regulating beta-1, 4-GalT-I expression in endothelial cells stimulated by TNF-alpha. PMID: 20619088
    20. The mutation of C80S introduces a fully occupied UDP binding site at the enzyme dimer interface that is observed to be dependent on the binding of H antigen acceptor analog. PMID: 20655926
    21. Golgi N-glycosyltransferases beta-1,2-N-acetylglucosaminyltransferase I, beta-1,2-N-acetylglucosaminyltransferase II, 1,4-galactosyltransferase I, and alpha-2,6-sialyltransferase I form both homo- and heterodimeric enzyme complexes in live cells PMID: 20378551
    22. found that both the long and short isoforms of beta-1,4-GalT-I were expressed in human CD4(+) T lymphocytes, and localized in the cytoplasm and on the plasma membrane. PMID: 20202494
    23. deficiency causes a new congenital disorder of glycosylation (CDG), designated type IId (CDG-IId), a severe neurologic disease characterized by hydrocephalus, myopathy, and blood-clotting defects PMID: 11901181
    24. TNFalpha modulates the glycosylation of endothelial cells by a mechanism that directly enhances the stability of beta4GalT-1 mRNA transcripts PMID: 15668241
    25. beta4Gal-T1 interacts preferentially with the 1,2-1,6-arm trisaccharide rather than with the 1,2-1,3-arm or 1,4-1,3-arm of a bi- or tri-antennary oligosaccharide chain of N-glycan PMID: 16157350
    26. Data show that expression of a hybrid enzyme of Arabidopsis thaliana xylosyltransferase and human beta-1,4-galactosyltransferase I in tobacco causes a reduction of N-glycans with potentially immunogenic core-bound xylose and fucose residues. PMID: 16675551
    27. These results demonstrated that cell surface beta1,4GT1 may negatively regulate cell survival possibly through inhibiting and modulating EGFR signaling pathway. PMID: 16786197
    28. Cycling between the trans-Golgi cisterna and the trans-Golgi network of galT is signal mediated. PMID: 17021253
    29. hepatitis B-induced GalT I expression might contribute to hepatitis B-mediated hepatocellular carcinoma development and progression PMID: 18929424
    30. Deoxygenated disaccharide analogs as specific inhibitors of beta1-4-galactosyltransferase 1 and selectin-mediated tumor metastasis. PMID: 19106107
    31. These results demonstrate that B3GNT1 and B4GALT1 physically associate in vitro and in cultured cells, providing insight into possible mechanisms for regulation of polyLacNAc production. PMID: 19261593

    顯示更多

    收起更多

  • 相關疾病:
    Congenital disorder of glycosylation 2D (CDG2D)
  • 亞細胞定位:
    [Isoform Long]: Golgi apparatus, Golgi stack membrane; Single-pass type II membrane protein. Cell membrane; Single-pass type II membrane protein. Cell surface. Cell projection, filopodium.; [Isoform Short]: Golgi apparatus, Golgi stack membrane; Single-pass type II membrane protein.; [Processed beta-1,4-galactosyltransferase 1]: Secreted.
  • 蛋白家族:
    Glycosyltransferase 7 family
  • 組織特異性:
    Ubiquitously expressed, but at very low levels in fetal and adult brain.
  • 數據庫鏈接:

    HGNC: 924

    OMIM: 137060

    KEGG: hsa:2683

    STRING: 9606.ENSP00000369055

    UniGene: Hs.272011



主站蜘蛛池模板: 一本一本久久a久久综合精品| 美丽的熟妇中文字幕| 男人的天堂在线视频| 精品人妻人人做人人爽| 亚洲综合色丁香婷婷六月图片| 成人h动漫无码网站久久| 国产精品乱码久久久久软件| 亚洲综合色无码| 国产精品精华液网站| 欧美成人免费全部| 国产综合有码无码视频在线 | 国产精品免费久久久久软件| 亚洲精品午夜国产va久久成人| 236宅宅理论片免费| 3p人妻少妇对白精彩视频| 天天综合色天天综合色h| 老男人久久青草av高清| 国产人妻精品午夜福利免费| 国产免费无遮挡吸奶头视频| 成在人线av无码免观看麻豆| av不卡国产在线观看| 99精品人妻少妇一区二区| 久久精品麻豆日日躁夜夜躁| 国产真实夫妇交换视频| 免费的av网站在线观看国产精品| 亚洲欧美综合精品二区| 无码免费一区二区三区| 337p粉嫩日本欧洲亚洲大胆| 桃花综合久久久久久久久久网| 国产一卡2卡3卡四卡精品国色无边| 亚洲精品无码ma在线观看| 明星性猛交ⅹxxx乱大交| а√中文在线资源库| 最新国产久免费视频在线观看| 中国熟女仑乱hd| 欧美va亚洲va在线观看| 亚洲人成无码网www电影麻豆| 欧美国产日韩a欧美在线观看| 国产在线一区二区香蕉 在线| 天堂а√在线最新版中文在线| 乱码午夜-极国产极内射|