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  4. Human Apolipoprotein O(APOO) ELISA kit

Human Apolipoprotein O(APOO) ELISA kit

  • 中文名稱：
    人載脂蛋白O(APOO)酶聯免疫試劑盒
  • 貨號：
    CSB-EL001948HU
  • 規格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產品詳情

  • 產品描述：
    人載脂蛋白O(APOO)酶聯免疫試劑盒（CSB-EL001948HU）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的APOO含量。試劑盒檢測范圍為0.312 ng/mL-20 ng/mL，該產品適用于基礎科研中APOO在肝臟、脂肪組織等代謝相關器官的表達分析，也可用于體外實驗模型（如細胞培養上清）或動物實驗中脂代謝通路的功能研究，為探索脂質穩態調控、能量代謝疾病機制及潛在干預策略提供可靠工具，嚴格限用于生命科學領域研究。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    APOO; FAM121B; MIC23; MIC26; My025; UNQ1866/PRO4302; MICOS complex subunit MIC26; Apolipoprotein O; MICOS complex subunit MIC23; Protein FAM121B
  • 縮寫：
    APOO
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    0.312 ng/mL-20 ng/mL
  • 靈敏度：
    0.08 ng/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Signal Transduction
  • 測定原理：
    quantitative
  • 測定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human APOO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:100 Average % 102    
    Range % 97-106    
    1:200 Average % 86    
    Range % 83-91    
    1:400 Average % 108    
    Range % 104-112    
    1:800 Average % 85    
    Range % 81-90    
  • 回收率：
    The recovery of human APOO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 98 97-106    
    EDTA plasma (n=4) 89 85-96    
                   
                   
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    ng/ml OD1 OD2 Average      
    20 0.122 0.126 0.124      
    10 0.207 0.215 0.211      
    5 0.326 0.331 0.329      
    2.5 0.553 0.549 0.551      
    1.25 0.846 0.854 0.850      
    0.625 1.197 1.200 1.199      
    0.312 1.586 1.598 1.592      
    0 2.387 2.400 2.394      
  • 數據處理：
  • 貨期：
    3-5 working days

產品評價

靶點詳情

  • 功能：
    Component of the MICOS complex, a large protein complex of the mitochondrial inner membrane that plays crucial roles in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane. Plays a crucial role in crista junction formation and mitochondrial function. Can promote cardiac lipotoxicity by enhancing mitochondrial respiration and fatty acid metabolism in cardiac myoblasts. Promotes cholesterol efflux from macrophage cells. Detected in HDL, LDL and VLDL. Secreted by a microsomal triglyceride transfer protein (MTTP)-dependent mechanism, probably as a VLDL-associated protein that is subsequently transferred to HDL.
  • 基因功能參考文獻：
    1. The human apolipoprotein MIC26 is a bona fide subunit of the MICOS complex. PMID: 25764979
    2. It is likely that apoO participates in fatty acid metabolism and the inflammatory response in HepG2 cells. PMID: 24341743
    3. APOO represents a link between impaired mitochondrial function and cardiomyopathy onset, and targeting APOO-dependent metabolic remodeling has potential as a strategy to adjust heart metabolism and protect the myocardium from impaired contractility. PMID: 24743151
    4. relationships between plasma apoO levels and high-sensitive C-reactive protein (hs-CRP) levels, as well as other lipid parameters in healthy subjects and patients with established acute coronary syndrome PMID: 22693255
    5. apolipoprotein O is the first chondroitin sulfate chain containing apolipoprotein PMID: 16956892

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  • 亞細胞定位：
    Mitochondrion inner membrane; Single-pass membrane protein. Secreted. Mitochondrion. Golgi apparatus membrane. Endoplasmic reticulum membrane.
  • 蛋白家族：
    Apolipoprotein O/MICOS complex subunit Mic27 family
  • 組織特異性：
    Expressed in all tissues examined. Up-regulated in diabetic heart.
  • 數據庫鏈接：

    HGNC: 28727

    OMIM: 300753

    KEGG: hsa:79135

    STRING: 9606.ENSP00000368528

    UniGene: Hs.495851



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