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Human Antithrombin III(AT III)ELISA Kit

  • 中文名稱:
    人抗凝血酶III(AT III)酶聯免疫試劑盒
  • 貨號:
    CSB-E17902h
  • 規格:
    96T/48T
  • 價格:
    ¥3600/¥2500
  • 其他:

產品詳情

  • 產品描述:
    人抗凝血酶III(AT III)酶聯免疫試劑盒(CSB-E17902h)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的SERPINC1含量。SERPINC1 是重要的靶點。它是一種絲氨酸蛋白酶抑制劑,參與凝血調控。深入研究其作用機制,有助于了解凝血相關疾病的發生發展過程,為尋找新的治療靶點和開發治療凝血異常的藥物等提供理論基礎。試劑盒檢測范圍為0.82 ng/mL-600 ng/mL,適用于心血管疾病模型研究、凝血機制探索、藥物開發中的抗凝活性評估等科研場景,為體外實驗提供靈敏可靠的分析工具。實驗操作標準化,支持多樣本類型檢測,滿足基礎醫學和生命科學領域對凝血系統調控機制的研究需求。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 別名:
    ANT3_HUMAN ELISA Kit; Antithrombin ELISA Kit; Antithrombin III ELISA Kit; Antithrombin-III ELISA Kit; AntithrombinIII ELISA Kit; AT 3 ELISA Kit; AT III ELISA Kit; AT3 ELISA Kit; AT3D ELISA Kit; ATIII ELISA Kit; Heparin cofactor I ELISA Kit; MGC22579 ELISA Kit; Serine (or cysteine) proteinase inhibitor clade C (antithrombin) member 1 ELISA Kit; Serine cysteine proteinase inhibitor clade C member 1 ELISA Kit; Serine proteinase inhibitor clade C member 1 ELISA Kit; Serpin C1 ELISA Kit; Serpin family C member 1 ELISA Kit; Serpin peptidase inhibitor clade C (antithrombin) member 1 ELISA Kit; SERPINC1 ELISA Kit; THPH7 ELISA Kit
  • 縮寫:
  • Uniprot No.:
  • 種屬:
    Homo sapiens (Human)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測范圍:
    0.82 ng/mL-600 ng/mL
  • 靈敏度:
    0.82 ng/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Cardiovascular
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

  • 線性度:

    To assess the linearity of the assay, samples were spiked with high concentrations of human AT III in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

     

    Sample

    Serum(n=4)

    1:1000

    Average %

    103

    Range %

    99-109

    1:2000

    Average %

    101

    Range %

    97-106

    1:4000

    Average %

    99

    Range %

    92-106

    1:8000

    Average %

    108

    Range %

    101-115

  • 回收率:

    The recovery of human AT III spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    96

    91-103

    EDTA plasma (n=4)

    101

    94-108

  • 標準曲線:

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    ng/ml

    OD1

    OD2

    Average

    Corrected

    600

    2.632

    2.624

    2.628

    2.426

    200

    2.272

    2.167

    2.220

    2.017

    66.67

    1.677

    1.728

    1.702

    1.500

    22.22

    1.055

    1.029

    1.042

    0.840

    7.4

    0.856

    0.821

    0.839

    0.636

    2.47

    0.526

    0.553

    0.540

    0.338

    0.82

    0.307

    0.294

    0.300

    0.098

    0

    0.199

    0.205

    0.202

     

  • 數據處理:
  • 貨期:
    3-5 working days

產品評價

靶點詳情

  • 功能:
    Most important serine protease inhibitor in plasma that regulates the blood coagulation cascade. AT-III inhibits thrombin, matriptase-3/TMPRSS7, as well as factors IXa, Xa and XIa. Its inhibitory activity is greatly enhanced in the presence of heparin.
  • 基因功能參考文獻:
    1. Molecular analysis may be the best method to identify antithrombin deficiency. Up to 80% of patients with antithrombin deficiency have SERPINC1 gene defects, mostly (90% of the 315 gene defects described so far) point mutations or small deletions or insertions affecting the 7 exons or flanking regions. [review] PMID: 30005274
    2. Laboratory tests and direct sequencing of PROC and SERPINC1 were conducted for the patient and his family members. Coagulation tests revealed that the patient presented type I antithrombin deficiency combined with decreased protein C activity resulting from a small insertion mutation c.848_849insGATGT in SERPINC1 and a short deletion variant c.572_574delAGA in PROC. PMID: 28861852
    3. Thirty-one members of a single family were included. Clinical data was collected regarding thrombotic history. The mutation was identified by direct sequencing of the SERPINC1 gene. HEK293 cells were transfected with wild type and mutant SERPINC1 plasmids. PMID: 28783511
    4. the first report of regulatory region polymorphisms in SERPINC1 gene in Indian population PMID: 27279637
    5. this study shows that risk for thrombosis is associated with the different SERPINC1 genotypes PMID: 28300866
    6. Report high levels of latent antithrombin in plasma from patients with antithrombin deficiency caused by mutations affecting the stability of the native conformation. PMID: 28229161
    7. Study identified one new small deletion within AT, which results in the loss of four amino acids (INEL) and is located at strand 3 of beta-sheet A, a region highly conserved in SERPINC1. This mutation leads to type I AT deficiency by promoting the intracellular retention of AT, which induces ER stress. PMID: 27708219
    8. It was our aim to identify mutations in SERPINC1 causing transient antithrombin deficiency. SERPINC1 was sequenced in 214 cases with a positive test for antithrombin deficiency, including 67 with no deficiency in the sample delivered to our laboratory. The p.Val30Glu mutation (Antithrombin Dublin) was identified in five out of these 67 cases, as well as in three out of 127 cases with other SERPINC1 mutations. PMID: 27098529
    9. different types of SERPINC1 mutations may play different roles in the development of VTE PMID: 27863268
    10. aberrant N-glycosylation causing a recessive or transient antithrombin deficiency is a new form of thrombophilia that does not have a SERPINC1 gene defect PMID: 27214821
    11. The results this study reveal several novel mutations to the already growing list of SERPINC1 mutations, thereby adding to our knowledge of the molecular background of antithrombin deficiency. PMID: 28317092
    12. Data indicate that all patients suffered from homozygous antithrombin (AT) deficiency caused by the mutation p.Leu131Phe in the AT gene (SERPINC1). PMID: 28361296
    13. Studies indicate that antithrombin III (ATIII) and its gene SerpinC1 may be related to many diseases, including hypertension and kidney diseases. PMID: 28424376
    14. The odds ratio of developing idiopathic fatal pulmonary embolism as a variant carrier for SERPINC1 is 144.2 (95% CI, 26.3-779.4; P = 1.7 x 10- 7). PMID: 28174134
    15. Nine patients (1.8%), [5% in arterial thrombosis and 0.8% in venous thrombosis] showed a missense variant in exon 5 i.e. p.Pro305His (c.1033 C > A); none of these patients showed the presence of any other variations in the gene. PMID: 27161325
    16. In Hungary, the founder mutation, ATBp3, is the most common Antithrombin deficiency PMID: 26748602
    17. Our studies of ATIII in-cell folding reveal a surprising, biased order of disulfide bond formation, with early formation of the C-terminal disulfide, before formation of the N-terminal disulfides, critical for folding to the active, metastable state PMID: 27222580
    18. Describe antibody specifically targeting a unique conformational epitope on antithrombin III beta conformation that blocks anticoagulation. PMID: 26581031
    19. This is the first case of pregnancy related stroke, associated with type-II heparin binding site antithrombin deficiency (c. 391C > T, p.Leu131Phe), that has been reported so far. A genetic analysis of the AT gene (SERPINC1) was performed. PMID: 26916305
    20. The present study highlights that AT physiological activities are strictly controlled not only by a core fucose at the reducing end but also by the high-mannose-type structures at the nonreducing end. The beta-form with the immature high-mannose type appears to function as a more potent anticoagulant than the AT typically found in human plasma, once it emerges in the blood. PMID: 26747427
    21. Elevated levels of circulating microparticles can play a role in carriers of mild and severe inherited thrombophilia resulting from antithrombin deficiency. PMID: 26354831
    22. The relevance of the vitamin D pathway on the regulation of SERPINC1 was confirmed in a cell model. PMID: 27003919
    23. The increased SERPINC1 SNP frequency among Han patients receiving heart surgery might contribute to the differences in their perioperative sensitivity to heparin PMID: 25361738
    24. Patients with low antithrombin III activities presented a higher risk of developing acute kidney injury after cardiac surgery. PMID: 26108065
    25. Letter/Case Report: novel antithrombin mutation resulting in antithrombin deficiency and arterial/venous thrombosis. PMID: 26177694
    26. Report antirhtombin III levels were negatively correlated with gestational age during third trimester of pregnancy, and fall further immediately after childbirth. PMID: 25087890
    27. This suggests that allosteric information propagation pathways are present even in the non-activated native form of antithrombin. PMID: 25483839
    28. analysis of mutations in SERPINC1 with a role in Hereditary antithrombin (AT) deficiency PMID: 25837307
    29. Polymorphisms in factor V and antithrombin III gene in recurrent pregnancy loss PMID: 25771983
    30. this is the first report of AT mutations in SERPINC1 gene in Indo-Aryan population where a novel point mutation p.T280A and a novel single nucleotide insertion g.13362_13363insA are reported. PMID: 25811371
    31. Selective disruption of exosite-mediated regulation of factor IX by heparin and antithrombin can be achieved with preserved or enhanced thrombin generation capacity. PMID: 25851619
    32. report of a large in-frame deletion causing antithrombin deficiency PMID: 25298121
    33. c.1058C>T variant in the SERPINC1 gene is pathogenic for antithrombin deficiency. PMID: 25522812
    34. We identified a novel hereditary mutation, g.1267G>A (p.A391T), in the AT gene, which reduces its heparin binding capacity and might be associated with resistance to heparin PMID: 25312341
    35. We posit that active site adduction is the mechanism of MGO-mediated inhibition of ATIII, and thus contributes to the underlying pathophysiology of the diabetic hypercoagulable state and complications thereof. PMID: 25307422
    36. prevalence of mutations in a cohort of pediatric patients with venous thromboembolism is reported PMID: 24966143
    37. AT-p.Ala416Pro mutation was responsible for type IIa AT deficiency in the family. PMID: 24583439
    38. genetic polymorphism affects endogenous thrombin potential among FV Leiden carriers PMID: 24226152
    39. The type of inherited AT defect modulates not only the risk of thromboembolism but also the localisation. PMID: 24196373
    40. data showed heterozygous mutations of c.2534C>T (R56C), c.13398C>A (A459D) and c.2703C>G (P112R) in the AT gene caused antithrombin (AT) deficiency in 3 unrelated Japanese pedigrees; findings suggest the A459D and P112R mutants are responsible for type I AT deficiency PMID: 23809926
    41. Mutation in SERPINC1 is associated with inherited homozygous antithrombin deficiency PMID: 24072242
    42. Rare double heterozygous mutations in antithrombin underlie hereditary thrombophilia in a Chinese family PMID: 23117546
    43. Data indicate that in patients on haemodialysis, thrombin-antithrombin (TAT) levels were increased and inversely correlated with primary assisted patency and secondary patency. PMID: 23844096
    44. The allosteric mechanism of activation of antithrombin as an inhibitor of factor IXa and factor Xa: heparin-independent full activation through mutations adjacent to helix D. PMID: 24068708
    45. Prevalence of inherited antithrombin mutations in thrombosis patients is higher than previously estimated. PMID: 23429250
    46. analysis of compound heterozygosty of SERPINC1 in antithrombin deficiency [case reports] PMID: 23329010
    47. A novel function for AT, which accelerates the modulation of FXa into the fibrinolytic form. PMID: 23416531
    48. Data suggest that plasma FVIIa-AT complex (coagulation factor VII-antithrombin III) is higher in portal vein thrombosis (PVT; without cirrhosis) than in healthy subjects; no difference in FVIIa-AT complex is observed in cirrhosis with/without PVT. PMID: 22958499
    49. A novel heterozygous mutation on exon 5 (c.1009C > T p.Q337X)of the SerpinC1 gene was identified in two half-siblings with neonatal cerebral sinus venous thrombosis. PMID: 22997155
    50. The serum ATIII level before hepatectomy in hepatocellular carcinoma is valuable to estimate the pathological background and predict postoperative liver failure/ dysfunction. PMID: 22353523

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  • 相關疾病:
    Antithrombin III deficiency (AT3D)
  • 亞細胞定位:
    Secreted, extracellular space.
  • 蛋白家族:
    Serpin family
  • 組織特異性:
    Found in plasma.
  • 數據庫鏈接:

    HGNC: 775

    OMIM: 107300

    KEGG: hsa:462

    STRING: 9606.ENSP00000356671

    UniGene: Hs.75599



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