在线日韩日本国产亚洲丨少妇伦子伦情品无吗丨欧美性猛交xxxx免费看蜜桃丨精品人妻系列无码一区二区三区丨亚洲精品无码不卡在线播放

Your Good Partner in Biology Research

  1. 首頁
  2. 試劑盒
  3. 酶聯免疫試劑盒
  4. Human Antibacterial Peptide LL-37 ELISA Kit

Human Antibacterial Peptide LL-37 ELISA Kit

  • 中文名稱：
    人抗菌肽LL-37酶聯免疫試劑盒
  • 貨號：
    CSB-E14948h
  • 規格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產品詳情

  • 產品描述：
    人抗菌肽LL-37酶聯免疫試劑盒（CSB-E14948h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的LL-37含量。LL-37是一種存在于人體中的抗菌肽，具有廣譜抗菌和抗腫瘤活性。其研究機制涉及抗菌、免疫調節、抗內毒素、趨化、刺激新生血管生成和誘導細胞凋亡等多方面。LL-37在多種胃腸道疾病中表達量變化，提示其在消化系疾病進展中發揮作用。試劑盒檢測范圍為0.156 ng/mL-10 ng/mL，適用于基礎科研中探索LL-37在感染模型、免疫調控機制或皮膚相關疾病中的動態變化，例如通過分析不同實驗條件下（如病原體刺激、藥物干預）生物樣本中LL-37的表達差異，為免疫學研究或治療策略開發提供數據支持本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 縮寫：
    LL-37
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    0.156 ng/mL-10 ng/mL
  • 靈敏度：
    0.039 ng/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Others
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human LL-37 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:5Average %85
    Range %80-92
    1:10Average %98
    Range %91-105
    1:20Average %98
    Range %92-110
    1:40Average %93
    Range %86-98
  • 回收率：
    The recovery of human LL-37 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9489-98
    EDTA plasma (n=4)10492-112
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    102.420 2.468 2.444 2.246
    51.875 1.895 1.885 1.687
    2.51.358 1.378 1.368 1.170
    1.250.941 0.956 0.949 0.751
    0.6250.552 0.589 0.571 0.373
    0.3120.431 0.453 0.442 0.244
    0.1560.378 0.389 0.384 0.186
    00.192 0.204 0.198 　
  • 本試劑盒所含材料：
    • A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-human antibacterial peptide LL-37 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
    • Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
    • One vial Biotin-labeled antibacterial peptide LL-37 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
    • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
    • One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
    • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
    • One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
    • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
    • One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
    • One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
    • Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
    • An instruction manual

    顯示更多

    收起更多

  • 本試劑盒不含材料：
    • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
    • An incubator that can provide stable incubation conditions up to 37°C±5°C.
    • Centrifuge
    • Vortex
    • Squirt bottle, manifold dispenser, or automated microplate washer
    • Absorbent paper for blotting the microtiter plate
    • 50-300ul multi-channel micropipette
    • Pipette tips
    • Single-channel micropipette with different ranges
    • 100ml and 500ml graduated cylinders
    • Deionized or distilled water
    • Timer
    • Test tubes for dilution

    顯示更多

    收起更多

  • 數據處理：
  • 貨期：
    3-5 working days

引用文獻

產品評價

相關問答

■ 常見問題解答
Q:

We want to use pbs buffer 7.4 as sample instead of serum/plasma etc. Would this affect the results? Can you give us any protocol/suggestion? We need an urgent reply:CSB-E14948h Human Antibacterial Peptide LL-37 ELISA Kit and CSB-E14187h Human β-defensins 3 ELISA Kit.

A:
Thanks for your inquiry.Sorry we did not ever tested PBS 7.4 as sample and we are not sure how it will go on.PBS is mainly composed of phosphate.
Normally,there is no detection target in PBS.May I know if you were trying to say that the customer want to use PBS 7.4 as the sample diluent,not the mentioned serum,plasma sample?The kit is equiped with sample diluent in it and we suggest the customer use the sample diluent in the kit directly.
If further questions,pls let us know.
Q:

I would like to test this ELISA for LL37. Would this ELISA can be used for the quantification of full length cathelicidin and LL37?

A:
Thanks for your inquiry. This kit detects LL-37.Any question, pls feel free to contact me.


主站蜘蛛池模板： 久久99国产精品久久99| 日韩精品一区二区av在线| 免费无码又爽又刺激毛片| 性一交一乱一乱一视频| 狠狠色狠狠色综合日日不卡 | 好硬好湿好爽好深视频| √天堂中文最新版在线中文| 欧美乱人伦人妻中文字幕| 国产猛男猛女超爽免费视频| 综合无码精品人妻一区二区三区 | 日韩人妻无码精品系列专区| 久久久久久夜精品精品免费啦| 18禁止看的免费污网站| 成人午夜国产内射主播| 日韩爆乳一区二区无码| 日韩免费无码专区精品观看| 夜夜影院未满十八勿进| 国产av一区二区三区天堂综合网| 亚洲另类欧美在线电影| 性高湖久久久久久久久| 四虎影视成人永久免费观看视频| 日日躁狠狠躁狠狠爱| 午夜爽爽爽男女免费观看影院| 色天使久久综合网天天| 国产精品久久久久不卡绿巨人| 日本一卡2卡三卡4卡免费网站| 国产亚洲精品第一综合麻豆| 精品国产三级在线观看| 亚洲人成无码区在线观看| 国产成人精品999视频| 国产精品成人无码免费| 久久久久有精品国产麻豆| 久久人人97超碰超国产| 成人毛片100免费观看| 18禁裸男晨勃露j毛免费观看| 亚洲中文字幕乱码av波多ji| 亚洲人成小说网站色| 日韩夜夜高潮夜夜爽无码| 亚洲国产精品国语在线| 女人摸下面自熨视频在线播放| 少妇爆乳无码专区|