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  4. Human Angiopoietin-related protein 7(ANGPTL7) ELISA kit

Human Angiopoietin-related protein 7(ANGPTL7) ELISA kit

  • 中文名稱：
    人血管生成素相關(guān)蛋白7(ANGPTL7)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-EL001715HU
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人血管生成素相關(guān)蛋白7(ANGPTL7)酶聯(lián)免疫試劑盒（CSB-EL001715HU）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的ANGPTL7含量。ANGPTL7是一種血管生成素樣蛋白，參與血管生成、炎癥反應(yīng)、內(nèi)皮細(xì)胞損傷和糖脂代謝。研究發(fā)現(xiàn)，ANGPTL7在阻塞性睡眠呼吸暫停低通氣綜合征（OSAHS）患者中表達(dá)升高，可能與OSAHS的發(fā)生發(fā)展相關(guān)，并通過(guò)調(diào)節(jié)炎癥反應(yīng)、內(nèi)皮損傷和脂代謝等機(jī)制影響疾病進(jìn)程。試劑盒檢測(cè)范圍為0.312 ng/mL-20 ng/mL，該產(chǎn)品適用于基礎(chǔ)醫(yī)學(xué)研究中ANGPTL7在血管發(fā)育、代謝調(diào)控及病理狀態(tài)下的動(dòng)態(tài)變化分析，也可用于藥物篩選模型中靶向干預(yù)效果的評(píng)估，為眼科疾病機(jī)制探索、代謝相關(guān)研究及生物標(biāo)志物開(kāi)發(fā)提供高效檢測(cè)方案。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書。
  • 別名：
    Angiopoietin like 7 ELISA Kit; Angiopoietin like factor (CDT6) ELISA Kit; Angiopoietin like factor ELISA Kit; Angiopoietin related protein 7 ELISA Kit; Angiopoietin-like factor ELISA Kit; Angiopoietin-like protein 7 ELISA Kit; Angiopoietin-related protein 7 ELISA Kit; ANGL7_HUMAN ELISA Kit; ANGPTL7 ELISA Kit; AngX ELISA Kit; CDT6 ELISA Kit; Cornea derived transcript 6 protein ELISA Kit; Cornea-derived transcript 6 protein ELISA Kit; dJ647M16.1 ELISA Kit; OTTHUMP00000001986 ELISA Kit; RP4-647M16.2 ELISA Kit
  • 縮寫：
    ANGPTL7
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    0.312 ng/mL-20 ng/mL
  • 靈敏度：
    0.078 ng/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Neuroscience
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human ANGPTL7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 99
    Range % 92-104
    1:2 Average % 87
    Range % 82-93
    1:4 Average % 92
    Range % 87-98
    1:8 Average % 101
    Range % 98-105
  • 回收率：
    The recovery of human ANGPTL7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 103 92-106
    EDTA plasma (n=4) 95 89-99
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected
    20 1.942 2.042 1.992 1.881
    10 1.239 1.149 1.194 1.083
    5 0.759 0.719 0.739 0.628
    2.5 0.453 0.464 0.459 0.348
    1.25 0.311 0.321 0.316 0.205
    0.625 0.212 0.223 0.218 0.107
    0.312 0.161 0.165 0.163 0.052
    0 0.110 0.111 0.111 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Has a role in the formation and organization of the extracellular matrix. In the eye, it functions as a mediator of dexamethasone-induced matrix deposition in the trabecular meshwork, the tissue responsible for the outflow of the ocular aqueous humor and for the maintenance of intraocular pressure. Is a negative regulator of angiogenesis in the cornea, and plays a major role in maintaining corneal avascularity and transparency.
  • 基因功能參考文獻(xiàn)：
    1. obesity increases the level of ANGPTL7 in both plasma and adipose tissue. Increased expression of ANGPTL7 might play a minor role in the regulation of TG level in obese subjects either directly or through interaction with other ANGPTL protein members. Physical exercise reduced the level of ANGPTL7 highlighting the potential for targeting this protein as a therapeutic target for regulating dyslipidemia. PMID: 28264047
    2. ANGPTL7, which is abundantly expressed in keratocytes, plays a major role in maintaining corneal avascularity and transparency. PMID: 25622036
    3. ANGPTL7 is markedly down regulated in metastatic liver cancer. PMID: 25854806
    4. Angiopoietin-like 7 is a novel pro-angiogenetic factor over-expressed in cancer PMID: 24903490
    5. Together with previous findings, these properties strengthen ANGPTL7's candidacy for the regulation of intraocular pressure and glaucoma. PMID: 21199193
    6. Induction of ANGPTL7 secretion by glaucoma stimuli and increased concentration of ANGPTL7 in glaucomatous AH suggest that ANGPTL7 is overexpressed in glaucoma. PMID: 18421092

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  • 亞細(xì)胞定位：
    Secreted.
  • 組織特異性：
    Higly expressed in the cornea (at protein level). Expression is restricted to the stromal layer. Also detected at the junction between the corneal stromal layer and the conjuctiva. Not detected in the sclera.
  • 數(shù)據(jù)庫(kù)鏈接：

    HGNC: 24078

    KEGG: hsa:10218

    STRING: 9606.ENSP00000366015

    UniGene: Hs.146559



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