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  4. Human ADAM DEC1(ADAMDEC1) ELISA kit

Human ADAM DEC1(ADAMDEC1) ELISA kit

  • 中文名稱：
    人ADAM DEC1(ADAMDEC1)酶聯免疫試劑盒
  • 貨號：
    CSB-EL001297HU
  • 規格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產品詳情

  • 產品描述：
    人ADAM DEC1(ADAMDEC1)酶聯免疫試劑盒（CSB-EL001297HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的ADAMDEC1含量。ADAMDEC1是一種金屬蛋白酶類基因，屬于ADAM家族的新成員。其在多種生物學過程中發揮作用，包括受精、神經發生、肌肉發育和免疫反應等。ADAMDEC1的研究有助于深入理解金屬蛋白酶類基因的生物學功能和疾病發生機制，為疾病的治療和預防提供新的思路和策略。試劑盒檢測范圍為62.5 pg/mL-4000 pg/mL，適用于體外研究ADAMDEC1在炎癥性疾病、腫瘤微環境等領域的表達調控及功能機制，可兼容多種生物樣本類型，為探索其分子作用提供靈敏可靠的工具本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    A disintegrin and metalloproteinase domain like protein decysin 1 ELISA Kit; A disintegrin and metalloproteinase domain-like protein decysin-1 ELISA Kit; ADAM DEC1 ELISA Kit; ADAM like decysin 1 ELISA Kit; ADAM like protein decysin 1 ELISA Kit; ADAM-like protein decysin-1 ELISA Kit; ADAMDEC 1 ELISA Kit; Adamdec1 ELISA Kit; ADEC1_HUMAN ELISA Kit; Decysin ELISA Kit; Disintegrin protease ELISA Kit; M12.219 ELISA Kit
  • 縮寫：
    ADAMDEC1
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    62.5 pg/mL-4000 pg/mL
  • 靈敏度：
    15.6 pg/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Cell Biology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human ADAMDEC1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:1 Average % 92    
    Range % 87-96    
    1:2 Average % 96    
    Range % 90-101    
    1:4 Average % 94    
    Range % 89-99    
    1:8 Average % 97    
    Range % 92-103    
  • 回收率：
    The recovery of human ADAMDEC1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 104 99-108    
    EDTA plasma (n=4) 94 89-98    
                   
                   
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    pg/ml OD1 OD2 Average Corrected    
    4000 2.224 2.364 2.294 2.141    
    2000 1.732 1.667 1.700 1.547    
    1000 1.127 1.071 1.099 0.946    
    500 0.702 0.713 0.708 0.555    
    250 0.431 0.442 0.437 0.284    
    125 0.314 0.321 0.318 0.165    
    62.5 0.246 0.252 0.249 0.096    
    0 0.152 0.154 0.153 　    
  • 數據處理：
  • 貨期：
    3-5 working days

產品評價

靶點詳情

  • 功能：
    May play an important role in the control of the immune response and during pregnancy.
  • 基因功能參考文獻：
    1. Data suggest that activated platelets release ADAMDEC1, which hydrolyzes pro-EGF (epidermal growth factor) to soluble, active HMW-EGF; proteolytic cleavage of pro-EGF first occurs at the C-terminal arginyl residue of the EGF domain; proteolysis is the regulated, rate-limiting step in generating soluble EGF from activated platelets. PMID: 28455445
    2. We hypothesize that these unique features of ADAMDEC1 may have evolved to escape from inhibition by endogenous metalloprotease inhibitors PMID: 25564618
    3. ADAMDEC1 functions as an active metalloprotease. PMID: 23754285
    4. molecular evolution by duplication in a metalloprotease gene cluster on chromosome 8p12 PMID: 12037602
    5. The ADAMDEC 1 haplotype may indicate an underlying mechanism for high FVIII levels in venous thromboembolism. PMID: 18449420
    6. Gene expression profiling performed on tissues obtained from pulmonary sarcoidosis patients identified ADAMDEC1 as a potential pathogenic mediator of lung damage and/or remodeling and may serve as a marker for this disease. PMID: 19218196

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  • 亞細胞定位：
    Secreted.
  • 組織特異性：
    Expressed highly in the small intestine and appendix, moderately in lymph node, mucosal lining of the colon, thymus, spleen and very weakly in the bone marrow. Predominantly expressed in dendritic cells (DC) of the germinal center. Weakly expressed in mon
  • 數據庫鏈接：

    HGNC: 16299

    OMIM: 606393

    KEGG: hsa:27299

    STRING: 9606.ENSP00000256412

    UniGene: Hs.521459



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