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中文名稱:人8-異構前列腺素F2α(8-epi-PGF2α)酶聯免疫試劑盒
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貨號:CSB-E09441h
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規格:96T/48T
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價格:¥3600/¥2500
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其他:
產品詳情
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產品描述:人8-異構前列腺素F2α(8-epi-PGF2α)酶聯免疫試劑盒(CSB-E09441h)為雙抗夾心法ELISA試劑盒,定量檢測血清、尿液、組織勻漿樣本中的8-epi-PGF2α含量。試劑盒檢測范圍為78 pg/mL-5000 pg/mL,該產品適用于體外研究樣本中氧化應激水平的動態監測,可應用于動物模型或細胞實驗中評估抗氧化治療效果、疾病進程中氧化損傷程度等科研場景,為探索氧化應激相關分子機制提供可靠工具。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
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別名:8-epi-prostaglandin F2α,8-epi-PGF2α,8-epi-PGF2 alpha
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縮寫:8-epi-PGF2α
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種屬:Homo sapiens (Human)
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樣本類型:serum, urine, tissue homogenates
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檢測范圍:78 pg/mL-5000 pg/mL
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靈敏度:19.5 pg/mL
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反應時間:1-5h
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樣本體積:50-100ul
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檢測波長:450 nm
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研究領域:Others
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測定原理:quantitative
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測定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human 8-epi-PGF2α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 96 Range % 87-103 1:2 Average % 86 Range % 82-93 1:4 Average % 100 Range % 95-103 1:8 Average % 93 Range % 84-100 -
回收率:
The recovery of human 8-epi-PGF2α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 96 90-103 -
標準曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. pg/ml OD1 OD2 Average Corrected 5000 2.965 2.897 2.931 2.820 2500 2.211 2.323 2.267 2.156 1250 1.599 1.566 1.583 1.472 625 0.811 0.802 0.807 0.696 312 0.505 0.474 0.490 0.379 156 0.311 0.301 0.306 0.195 78 0.251 0.234 0.243 0.132 0 0.113 0.109 0.111 -
數據處理:
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貨期:3-5 working days
引用文獻
- Association between short-term exposure to PM2. 5 and nasal microbiota dysbiosis, inflammation and oxidative stress: A panel study of healthy young adults W Li,Ecotoxicology and environmental safety,2023
- Bradykinin Protects Human Endothelial Progenitor Cells from High-Glucose-Induced Senescence through B2 Receptor-Mediated Activation of the Akt/eNOS Signalling Pathway Yuehuan Wu,Journal of Diabetes Research,2021
- The Value of 8-iso Prostaglandin F2 Alpha and Superoxide Dismutase Activity as a Clinical Indicator of Oxidative Stress in Type II Diabetes Mellitus MORSI, et al,Journal of Clinical & Diagnostic Research,2018
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