Human 6-hydroxymelatonin sulfate (6HMS) ELISA kit

產(chǎn)品詳情

文獻(xiàn)引用 (2) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

人6-羥基硫酸褪黑素(6HMS)酶聯(lián)免疫試劑盒（CSB-EQ027870HU）為競(jìng)爭(zhēng)法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的6HMS含量。人6-羥基硫酸褪黑素（6HMS）是褪黑素的代謝產(chǎn)物，參與調(diào)節(jié)生物節(jié)律和抗氧化作用。研究發(fā)現(xiàn)，6HMS在妊娠期高血壓疾病（HDCP）中發(fā)揮重要作用，通過調(diào)節(jié)胎盤氧化應(yīng)激水平和內(nèi)皮功能，參與HDCP的發(fā)病機(jī)制。試劑盒檢測(cè)范圍為10 pg/mL- 400 pg/mL，可應(yīng)用于褪黑素代謝通路研究、神經(jīng)內(nèi)分泌系統(tǒng)功能分析，或探索6HMS在睡眠障礙、神經(jīng)退行性疾病及免疫調(diào)節(jié)中的潛在作用機(jī)制，為體外實(shí)驗(yàn)提供穩(wěn)定可靠的檢測(cè)工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

6HMS
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates
檢測(cè)范圍：

10 pg/mL- 400 pg/mL
靈敏度：

10 pg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Others
測(cè)定原理：

quantitative
測(cè)定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human 6HMS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	92-98
1:2	Average %	95
	Range %	87-105
1:4	Average %	96
	Range %	89-102
1:8	Average %	92
	Range %	88-96

回收率：

The recovery of human 6HMS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	89-100
EDTA plasma (n=4)	95	91-101

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.