Human 5-Hydroxytryptamine,5HT ELISA Kit

產(chǎn)品詳情

文獻引用 (2) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

人5羥色胺(5-HT)酶聯(lián)免疫試劑盒（CSB-E08363h）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的5HT含量。5HT即血清素，是一種重要的神經(jīng)遞質(zhì)。其在調(diào)節(jié)情緒、睡眠、食欲等方面發(fā)揮關鍵作用。研究機制上，主要通過與不同受體結(jié)合，影響神經(jīng)信號傳導來調(diào)節(jié)生理和心理功能，對其研究有助于揭示相關疾病發(fā)病機制及開發(fā)新藥。試劑盒檢測范圍為4 ng/mL-800 ng/mL，特別適用于神經(jīng)遞質(zhì)代謝研究、精神疾病動物模型分析、藥理學實驗中的藥效評估等科研領域，例如探究抑郁癥或腸易激綜合征模型中5-HT的動態(tài)變化，或篩選影響5-HT合成/代謝的化合物。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

5HT
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

4 ng/mL-800 ng/mL
靈敏度：

2 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Neuroscience
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human 5-HT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	96
	Range %	92-102
1:2	Average %	94
	Range %	91-98
1:4	Average %	85
	Range %	80-89
1:8	Average %	95
	Range %	89-99

回收率：

The recovery of human 5-HT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-99
EDTA plasma (n=4)	89	85-96

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.