Human 3-nitrotyrosine (3-NT) ELISA Kit
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中文名稱:人3-硝基酪氨酸酶聯(lián)免疫試劑盒
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貨號(hào):CSB-E14324h
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規(guī)格:96T/48T
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價(jià)格:¥3600/¥2500
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其他:
產(chǎn)品詳情
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產(chǎn)品描述:人3-硝基酪氨酸酶聯(lián)免疫試劑盒(CSB-E14324h)為雙抗夾心法ELISA試劑盒,定量檢測(cè)血清、血漿、組織勻漿樣本中的3-NT含量。3-NT(神經(jīng)營(yíng)養(yǎng)素-3)是一種由人類NTF3基因編碼的蛋白質(zhì),屬于神經(jīng)生長(zhǎng)因子(NGF)家族。它對(duì)中樞神經(jīng)系統(tǒng)和周圍神經(jīng)系統(tǒng)的特定神經(jīng)元有調(diào)控活性,支持神經(jīng)細(xì)胞的生存和分化,并促進(jìn)新神經(jīng)元的生長(zhǎng)和分化。3-NT通過與其受體TrkC和TrkB結(jié)合,激活細(xì)胞信號(hào)傳導(dǎo),影響神經(jīng)發(fā)生和神經(jīng)元功能。試劑盒檢測(cè)范圍為0.156 ng/mL-10 ng/mL,適用于體外研究領(lǐng)域,如氧化應(yīng)激相關(guān)疾病模型的分子機(jī)制探索、抗氧化藥物篩選及藥效評(píng)估等實(shí)驗(yàn)場(chǎng)景本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
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縮寫:3-NT
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種屬:Homo sapiens (Human)
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樣本類型:serum, plasma, tissue homogenates
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檢測(cè)范圍:0.156 ng/mL-10 ng/mL
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靈敏度:0.039 ng/mL
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反應(yīng)時(shí)間:1-5h
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樣本體積:50-100ul
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檢測(cè)波長(zhǎng):450 nm
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研究領(lǐng)域:Signal Transduction
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測(cè)定原理:quantitative
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測(cè)定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human 3-NT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 97 Range % 92-102 1:2 Average % 90 Range % 82-98 1:4 Average % 104 Range % 99-111 1:8 Average % 86 Range % 82-96 -
回收率:
The recovery of human 3-NT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 90 85-97 EDTA plasma (n=4) 91 85-96 -
標(biāo)準(zhǔn)曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. ng/ml OD1 OD2 Average Corrected 10 2.655 2.752 2.704 2.553 5 2.111 2.275 2.193 2.042 2.5 1.486 1.465 1.476 1.325 1.25 0.769 0.799 0.784 0.633 0.625 0.485 0.462 0.474 0.323 0.312 0.372 0.399 0.386 0.235 0.156 0.251 0.264 0.258 0.107 0 0.152 0.150 0.151 -
數(shù)據(jù)處理:
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貨期:3-5 working days
引用文獻(xiàn)
- Evaluation of the relationship between cardiac autonomic function and glucose variability and HOMA-IR in prediabetes R Dimova,Diabetes and Vascular Disease Research,2020
- Nitration-mediated deficiency of cystathionine β-synthase activity accelerates the progression of hyperhomocysteinemia Wang H.et al,Free Radic Biol Med,2017
- β-glucans: ex vivo inflammatory and oxidative stress results after pasta intake. Barera A.et al,Immun Ageing.,2016
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