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  4. Human β-defensins 2 ELISA Kit

Human β-defensins 2 ELISA Kit

  • 中文名稱：
    人β-防御素2酶聯免疫試劑盒
  • 貨號：
    CSB-E13201h
  • 規格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產品詳情

  • 產品描述：
    人β-防御素2酶聯免疫試劑盒（CSB-E13201h）為雙抗夾心法ELISA試劑盒，定量檢測血清、尿液、組織培養上清液、組織勻漿樣本中的DEFB4含量。DEFB4即β-防御素 4，是一種內源性抗菌肽。它在機體固有免疫中起關鍵作用，具有廣譜抗菌活性，能抵御細菌、真菌等病原體。其研究機制主要聚焦在它與病原體膜相互作用、免疫調節功能及信號通路激活等方面，為抗感染治療提供新思路。試劑盒檢測范圍為62.5 pg/mL-4000 pg/mL，該產品適用于科研領域對感染性病原體應答機制、慢性炎癥性疾病（如銀屑病、特應性皮炎）的病理研究，也可用于評估藥物或生物制劑對 hBD - 2 分泌的調控作用，為免疫調節、黏膜屏障功能及感染免疫方向的體外實驗提供可靠工具。樣本兼容性廣泛，支持多種生物樣本類型的穩定檢測，操作流程標準化，適用于實驗室常規科研需求。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    BD 2 ELISA Kit; BD-2 ELISA Kit; BD2 ELISA Kit; beta 2 ELISA Kit; beta 2 Defensin ELISA Kit; Beta defensin 2 ELISA Kit; Beta defensin 4B ELISA Kit; Beta-defensin 2 ELISA Kit; Beta-defensin 4A ELISA Kit; DEF B2 ELISA Kit; DEF B4 ELISA Kit; DEFB 102 ELISA Kit; DEFB 2 ELISA Kit; DEFB 4 ELISA Kit; DEFB102 ELISA Kit; DEFB2 ELISA Kit; DEFB2; formerly ELISA Kit; DEFB4 ELISA Kit; DEFB4; formerly ELISA Kit; DEFB4B ELISA Kit; DEFB4P ELISA Kit; Defensin ELISA Kit; Defensin beta 2 ELISA Kit; Defensin; beta 4 ELISA Kit; Defensin; beta 4; pseudogene ELISA Kit; Defensin; beta 4A ELISA Kit; Defensin; beta 4B ELISA Kit; Defensin; beta; 2; formerly ELISA Kit; Defensin; beta; 4; formerly ELISA Kit; DFB4A_HUMAN ELISA Kit; HBD 2 ELISA Kit; hBD-2 ELISA Kit; HBD2 ELISA Kit; mBD-2 ELISA Kit; SAP 1 ELISA Kit; SAP1 ELISA Kit; Skin antimicrobial peptide 1 ELISA Kit; Skin-antimicrobial peptide 1 ELISA Kit
  • 縮寫：
    DEFB4
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, urine, cell culture supernates, tissue homogenates
  • 檢測范圍：
    62.5 pg/mL-4000 pg/mL
  • 靈敏度：
    15.6 pg/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Microbiology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human β-defensins 2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %87
    Range %81-92
    1:2Average %94
    Range %90-100
    1:4Average %98
    Range %91-110
    1:8Average %91
    Range %85-98
  • 回收率：
    The recovery of human β-defensins 2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9389-98
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    40002.821 2.752 2.787 2.689
    20002.182 2.076 2.129 2.031
    10001.438 1.364 1.401 1.303
    5000.802 0.793 0.798 0.700
    2500.471 0.437 0.454 0.356
    1250.302 0.290 0.296 0.198
    62.50.243 0.229 0.236 0.138
    00.099 0.097 0.098 　
  • 本試劑盒所含材料：
    • A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human DEFB4 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
    • Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
    • One vial Biotin-labeled DEFB4 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
    • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
    • One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
    • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
    • One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
    • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
    • One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
    • One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
    • Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
    • An instruction manual

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  • 本試劑盒不含材料：
    • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
    • An incubator can provide stable incubation conditions up to 37°C±5°C.
    • Centrifuge
    • Vortex
    • Squirt bottle, manifold dispenser, or automated microplate washer
    • Absorbent paper for blotting the microtiter plate
    • 50-300ul multi-channel micropipette
    • Pipette tips
    • Single-channel micropipette with different ranges
    • 100ml and 500ml graduated cylinders
    • Deionized or distilled water
    • Timer
    • Test tubes for dilution

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  • 數據處理：
  • 貨期：
    3-5 working days

引用文獻

產品評價

靶點詳情

  • 功能：
    Exhibits antimicrobial activity against Gram-negative bacteria and Gram-positive bacteria. May act as a ligand for C-C chemokine receptor CCR6. Can bind to both human and mouse CCR6 and induce chemotactic activity of CCR6-expressing cells.
  • 基因功能參考文獻：
    1. The encoded peptide has antimicrobial activity against E.coli, S. aureus, and P. aeruginosa. PMID: 9727055
  • 亞細胞定位：
    Secreted.
  • 蛋白家族：
    Beta-defensin family, LAP/TAP subfamily
  • 組織特異性：
    Expressed in the skin and respiratory tract.
  • 數據庫鏈接：

    HGNC: 2767

    OMIM: 602215

    KEGG: hsa:100289462

    STRING: 9606.ENSP00000424598

    UniGene: Hs.105924



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