Horse Erythropoietin(EPO) ELISA kit

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產品詳情

產品描述：

馬紅細胞生成素(EPO)酶聯免疫試劑盒（CSB-EL007743HO）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細胞裂解物樣本中的EPO含量。EPO（促紅細胞生成素）是一種糖蛋白激素，主要在腎臟產生，負責調節紅細胞生成。其研究機制涉及EPO與受體結合，通過JAK2-STAT5通路促進紅細胞生成，并在類風濕關節炎等疾病中發揮重要作用。試劑盒檢測范圍為0.156 ng/mL-10 ng/mL，適用于科研領域探索EPO在生理或病理條件下的表達規律，例如研究貧血相關疾病機制、慢性腎病引起的紅細胞生成異常、腫瘤微環境對造血功能的干擾，或評估藥物及生物活性分子對EPO信號通路的影響。可為體外實驗提供精準的定量數據支持，助力于血液學、腫瘤生物學及代謝調控等領域的分子機制研究。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

EPOErythropoietin ELISA Kit
縮寫：

EPO
Uniprot No.：

Q867B1
種屬：

Equus caballus (Horse)
樣本類型：

serum, plasma, tissue homogenates, cell lysates
檢測范圍：

0.156 ng/mL-10 ng/mL
靈敏度：

0.039 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of horse EPO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	87
	Range %	82-91
1:2	Average %	84
	Range %	80-88
1:4	Average %	95
	Range %	90-100
1:8	Average %	98
	Range %	94-102

回收率：

The recovery of horse EPO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	85-97
EDTA plasma (n=4)	106	101-113

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Hormone involved in the regulation of erythrocyte proliferation and differentiation and the maintenance of a physiological level of circulating erythrocyte mass. Binds to EPOR leading to EPOR dimerization and JAK2 activation thereby activating specific downstream effectors, including STAT1 and STAT3.
亞細胞定位：

Secreted.
蛋白家族：

EPO/TPO family
數據庫鏈接：

KEGG: ecb:100033849

STRING: 9796.ENSECAP00000008707

UniGene: Eca.12758