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  4. Guinea pig monocyte chemotactic protein 1,MCP1 ELISA Kit

Guinea pig monocyte chemotactic protein 1,MCP1 ELISA Kit

  • 中文名稱：
    豚鼠單核細(xì)胞趨化蛋白1(MCP-1/CCL2/MCAF)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E13559Gp
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3800/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    豚鼠單核細(xì)胞趨化蛋白1(MCP-1/CCL2/MCAF)酶聯(lián)免疫試劑盒（CSB-E13559Gp）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的CCL2含量。CCL2是一種趨化因子，在免疫調(diào)節(jié)、炎癥反應(yīng)等生理病理過(guò)程中扮演重要角色。研究發(fā)現(xiàn)它能募集單核細(xì)胞等免疫細(xì)胞到炎癥部位。其異常表達(dá)與腫瘤進(jìn)展、心血管疾病等密切相關(guān)，探索CCL2作用機(jī)制有助于疾病治療新策略的開發(fā)。試劑盒檢測(cè)范圍為23.5 pg/mL-1500 pg/mL，可應(yīng)用于炎癥機(jī)制研究、免疫調(diào)節(jié)實(shí)驗(yàn)、疾病模型建立等基礎(chǔ)研究領(lǐng)域。例如在豚鼠感染模型或自身免疫性疾病模型中，可通過(guò)監(jiān)測(cè)血清或組織樣本中MCP - 1的動(dòng)態(tài)變化，揭示疾病進(jìn)程中趨化因子的調(diào)控規(guī)律。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說(shuō)明書。
  • 別名：
    CCL2 ELISA Kit; MCP1 ELISA Kit; SCYA2C-C motif chemokine 2 ELISA Kit; Monocyte chemoattractant protein 1 ELISA Kit; Monocyte chemotactic protein 1 ELISA Kit; MCP-1 ELISA Kit; Small-inducible cytokine A2 ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Guinea Sus scrofa (Pig)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    23.5 pg/mL-1500 pg/mL
  • 靈敏度：
    5.8 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of guinea pig MCP-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %91
    Range %86-95
    1:2Average %102
    Range %97-107
    1:4Average %91
    Range %85-97
    1:8Average %97
    Range %91-103
  • 回收率：
    The recovery of guinea pig MCP-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9589-98
    EDTA plasma (n=4)9387-98
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    15001.857 1.902 1.880 1.680
    7501.345 1.403 1.374 1.174
    3750.981 0.954 0.968 0.768
    1870.748 0.700 0.724 0.524
    940.511 0.532 0.522 0.322
    470.416 0.426 0.421 0.221
    23.50.289 0.281 0.285 0.085
    00.201 0.199 0.200
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Acts as a ligand for C-C chemokine receptor CCR2. Signals through binding and activation of CCR2 and induces a strong chemotactic response and mobilization of intracellular calcium ions. Exhibits a chemotactic activity for monocytes and basophils but not neutrophils or eosinophils. Plays an important role in mediating peripheral nerve injury-induced neuropathic pain. Increases NMDA-mediated synaptic transmission in both dopamine D1 and D2 receptor-containing neurons, which may be caused by MAPK/ERK-dependent phosphorylation of GRIN2B/NMDAR2B.
  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    Intercrine beta (chemokine CC) family
  • 數(shù)據(jù)庫(kù)鏈接：


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