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中文名稱:豚鼠白介素13(IL-13)酶聯免疫試劑盒
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貨號:CSB-E12916Gp
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規格:96T/48T
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價格:¥3800/¥2500
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其他:
產品詳情
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產品描述:豚鼠白介素13(IL-13)酶聯免疫試劑盒(CSB-E12916Gp)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的IL13含量。IL13是一種免疫調節細胞因子,參與過敏性炎癥和癌癥的發病機制。主要由活化的TH2細胞分泌,與多種細胞相互作用,調節免疫反應。研究IL13有助于了解相關疾病的發生和發展,為疾病治療提供新靶點。試劑盒檢測范圍為7.5 pg/mL-300 pg/mL,適用于豚鼠感染模型、呼吸道炎癥研究、免疫調節藥物開發等科研場景,能夠穩定檢測包括肺組織勻漿在內的多種生物樣本,為探索IL-13在免疫調控網絡中的作用及評估實驗性治療方案的生物學效應提供可靠工具。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
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縮寫:IL-13
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Uniprot No.:
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種屬:Guinea Sus scrofa (Pig)
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樣本類型:serum, plasma, tissue homogenates
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檢測范圍:7.5 pg/mL-300 pg/mL
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靈敏度:3.75 pg/mL
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反應時間:1-5h
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樣本體積:50-100ul
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檢測波長:450 nm
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研究領域:Immunology
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測定原理:quantitative
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測定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<15% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<15% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of Guinea pig IL-13 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 88 Range % 83-92 1:2 Average % 95 Range % 85-105 1:4 Average % 93 Range % 86-102 1:8 Average % 97 Range % 92-110 -
回收率:
The recovery of Guinea pig IL-13 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 96 91-103 EDTA plasma (n=4) 93 86-99 -
標準曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. pg/ml OD1 OD2 Average 300 2.244 2.267 2.256 150 1.173 1.161 1.167 75 0.628 0.650 0.639 37.5 0.359 0.343 0.351 15 0.152 0.151 0.152 7.5 0.083 0.079 0.081 -
數據處理:
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貨期:3-5 working days
引用文獻
- Altered metabolites in guinea pigs with allergic asthma after acupoint sticking therapy: new insights from a metabolomics approach Shi-jian Xiang.et al,Phytomedicine,2018
- Therapeutic effects of naringin in a guinea pig model of ovalbumin-induced cough-variant asthma Jiao HY. et al,Pulm Pharmacol Ther,2015
- Increased arginase activity contributes to airway remodelling in chronic allergic asthma /,european respiratory journal,2011
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