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  4. Guinea pig interleukin 10,IL-10 ELISA Kit

Guinea pig interleukin 10,IL-10 ELISA Kit

  • 中文名稱：
    豚鼠白介素10(IL-10)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E12915Gp
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3800/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    豚鼠白介素10(IL-10)酶聯(lián)免疫試劑盒（CSB-E12915Gp）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的IL10含量。IL10是重要的細(xì)胞因子，其背景在于它具有免疫調(diào)節(jié)作用，能抑制多種促炎因子釋放。研究機(jī)制方面，主要通過與其受體結(jié)合，激活下游信號(hào)通路，調(diào)控免疫細(xì)胞活性和功能，從而在炎癥、自身免疫病等疾病研究中備受關(guān)注。試劑盒檢測(cè)范圍為31.25 pg/mL-2000 pg/mL，適用于科研場(chǎng)景下豚鼠感染性疾病模型、過敏反應(yīng)研究、免疫調(diào)節(jié)劑開發(fā)等領(lǐng)域的IL-10表達(dá)水平分析，尤其適合評(píng)估實(shí)驗(yàn)藥物或生物制劑對(duì)免疫通路的影響。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    IL10Interleukin-10 ELISA Kit; IL-10 ELISA Kit; Cytokine synthesis inhibitory factor ELISA Kit; CSIF ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Guinea Sus scrofa (Pig)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    31.25 pg/mL-2000 pg/mL
  • 靈敏度：
    7.81 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of guinea pig IL-10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %90
    Range %85-95
    1:2Average %96
    Range %91-103
    1:4Average %95
    Range %89-100
    1:8Average %97
    Range %91-100
  • 回收率：
    The recovery of guinea pig IL-10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9689-101
    EDTA plasma (n=4)9791-105
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    20002.894 2.903 2.899 2.780
    10002.107 2.115 2.111 1.992
    5001.233 1.241 1.237 1.118
    2500.756 0.767 0.762 0.643
    1250.443 0.451 0.447 0.328
    62.50.289 0.299 0.294 0.175
    31.250.184 0.197 0.191 0.072
    00.118 0.120 0.119 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3. In turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators. Targets antigen-presenting cells (APCs) such as macrophages and monocytes and inhibits their release of pro-inflammatory cytokines including granulocyte-macrophage colony-stimulating factor /GM-CSF, granulocyte colony-stimulating factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF-alpha. Interferes also with antigen presentation by reducing the expression of MHC-class II and co-stimulatory molecules, thereby inhibiting their ability to induce T cell activation. In addition, controls the inflammatory response of macrophages by reprogramming essential metabolic pathways including mTOR signaling.
  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    IL-10 family