Guinea pig Immunoglobulin G,IgG ELISA Kit

產(chǎn)品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

豚鼠免疫球蛋白G(IgG)酶聯(lián)免疫試劑盒（CSB-E10125Gu）為競爭法ELISA試劑盒，定量檢測血清、血漿樣本中的IgG含量。IgG是人體免疫系統(tǒng)中的主要抗體，由B細(xì)胞產(chǎn)生，具有抗菌、抗病毒和中和毒素等功能。研究機(jī)制涉及B細(xì)胞分化為漿細(xì)胞，分泌IgG抗體，以及抗體與抗原結(jié)合后的免疫反應(yīng)。近年來，研究發(fā)現(xiàn)腫瘤細(xì)胞也能合成IgG，稱為腫瘤來源免疫球蛋白（cancer-IgG），其表達(dá)與腫瘤的侵襲和轉(zhuǎn)移密切相關(guān)。試劑盒檢測范圍為62.5 μg/mL-1000 μg/mL，靈敏度為62.5 μg/mL。該產(chǎn)品適用于科研領(lǐng)域，如感染性疾病模型中免疫應(yīng)答動(dòng)態(tài)監(jiān)測、疫苗研發(fā)中抗體效價(jià)評(píng)估，或基礎(chǔ)免疫學(xué)研究中豚鼠體液免疫功能分析。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

IgG
種屬：

Guinea Sus scrofa (Pig)
樣本類型：

serum, plasma
檢測范圍：

62.5 μg/mL-1000 μg/mL
靈敏度：

62.5 μg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Immunology
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of guinea pig IgG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:10	Average %	99
	Range %	95-103
1:20	Average %	94
	Range %	91-98
1:40	Average %	95
	Range %	90-100
1:80	Average %	92
	Range %	85-96

回收率：

The recovery of guinea pig IgG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	92-104
EDTA plasma (n=4)	96	84-110

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

最新研究進(jìn)展：

IgG 參與感染病毒和細(xì)菌的清除：IgG 抗體可以與病毒或細(xì)菌的表面蛋白結(jié)合，促進(jìn)免疫細(xì)胞清除它們。近期的研究表明，IgG 抗體可以通過與天然殺傷性 T 細(xì)胞結(jié)合，增強(qiáng)它們清除病原體的能力。
研究進(jìn)展表明，IgG 在疾病預(yù)防和治療中具有重要作用，并且對(duì)其生物學(xué)和作用機(jī)制的研究仍在不斷深入。