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中文名稱:山羊促性腺激素釋放激素(GnRH)酶聯免疫試劑盒
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貨號:CSB-E13276G
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規格:96T/48T
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價格:¥4200/¥3000
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其他:
產品詳情
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產品描述:山羊促性腺激素釋放激素(GnRH)酶聯免疫試劑盒(CSB-E13276G)為競爭法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的GnRH含量。GnRH(促性腺激素釋放激素)是下丘腦分泌的十肽激素,在生殖調節中起關鍵作用。其作用機制為脈沖式分泌,刺激垂體前葉合成和釋放促性腺激素,進而調控性腺功能。對GnRH的研究有助于了解生殖生理及攻克相關疾病。試劑盒檢測范圍為2.5 pg/mL-500 pg/mL,該產品可廣泛應用于山羊等反芻動物的生殖周期研究、繁殖調控機制探索、激素水平動態監測以及環境內分泌干擾物評估等科研場景。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
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縮寫:GnRH
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Uniprot No.:
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種屬:Capra hircus (Goat)
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樣本類型:serum, plasma, tissue homogenates
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檢測范圍:2.5 pg/mL-500 pg/mL
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靈敏度:1.25 pg/mL
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反應時間:1-5h
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樣本體積:50-100ul
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檢測波長:450 nm
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研究領域:Signal Transduction
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測定原理:quantitative
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測定方法:Competitive
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精密度:
Intra-assay Precision (Precision within an assay): CV%<15% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<15% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of goat GnRH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 95 Range % 90-99 1:2 Average % 95 Range % 88-102 1:4 Average % 94 Range % 89-99 1:8 Average % 95 Range % 89-103 -
回收率:
The recovery of goat GnRH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 95 88-102 EDTA plasma (n=4) 97 91-104 -
標準曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. pg/ml OD1 OD2 Average 0 2.557 2.458 2.508 2.5 1.495 1.481 1.488 10 1.053 1.045 1.049 40 0.650 0.636 0.643 125 0.408 0.392 0.400 500 0.222 0.214 0.218 -
數據處理:
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貨期:3-5 working days
引用文獻
- Effect of central administration of indomethacin on anandamide-induced GnRH/LH secretion in the hypothalamus of anoestrous ewes D Tomaszewska-Zaremba,Journal of Veterinary Research,2024
- Does kisspeptin participate in GABA-mediated modulation of GnRH and GnRH receptor biosynthesis in the hypothalamic-pituitary unit of follicular-phase ewes? Magdalena OlgaCiechanowska, et al,Pharmacological reports,2019
- Effect of CD14/TLR4 antagonist on GnRH/LH secretion in ewe during central inflammation induced by intracerebroventricular administration of LPS Karolina Haziak.et al,Journal of Animal Science and Biotechnology,2018
- Hypothalamic-pituitary GnRH/LH axis activity is affected by salsolinol in sheep during lactation: Effects of intracerebroventricular infusions of salsolinol and its antagonizing analogue. Marciniak E.et al,Theriogenology.,2016
- Central Injection of Exogenous IL-1ß in the Control Activities of Hypothalamic??ituitary??onadal Axis in Anestrous Ewes AP Herman et al,Reproduction in Domestic Animals,2012
- The effect of rivastigmine on the LPS-induced suppression of GnRH/LH secretion during the follicular phase of the estrous cycle in ewes Herman AP et al,Anim Reprod Sci.,2013
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