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Goat Interleukin 10,IL-10 ELISA Kit

  • 中文名稱:
    山羊白介素10(IL-10)酶聯(lián)免疫試劑盒
  • 貨號:
    CSB-E09809g
  • 規(guī)格:
    96T/48T
  • 價格:
    ¥4200/¥3000
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
    山羊白介素10(IL-10)酶聯(lián)免疫試劑盒(CSB-E09809g)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的IL10含量。IL10,又稱白細胞介素-10,是一種多效性細胞因子,主要由單核細胞和T細胞產(chǎn)生。它具有抗炎和免疫調(diào)節(jié)功能,通過抑制Th1細胞因子和抗原呈遞細胞的功能來發(fā)揮其作用。IL10的研究機制涉及信號轉(zhuǎn)導和轉(zhuǎn)錄激活因子STAT1、STAT3的激活,以及與其他細胞因子的相互作用。試劑盒檢測范圍為31.25 pg/mL-2000 pg/mL,適用于科研領(lǐng)域研究山羊模型中的免疫應答機制、炎癥性疾病進展評估或疫苗開發(fā)中的免疫調(diào)節(jié)分析。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 縮寫:
    IL-10
  • Uniprot No.:
  • 種屬:
    Capra hircus (Goat)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測范圍:
    31.25 pg/mL-2000 pg/mL
  • 靈敏度:
    7.8 pg/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領(lǐng)域:
    Immunology
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of goat IL-10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %82
    Range %80-85
    1:2Average %102
    Range %97-107
    1:4Average %89
    Range %84-93
    1:8Average %107
    Range %103-111
  • 回收率:
    The recovery of goat IL-10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9490-98
    EDTA plasma (n=4)8580-90
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    20002.725 2.768 2.747 2.615
    10002.059 2.000 2.030 1.898
    5001.321 1.368 1.345 1.213
    2500.741 0.758 0.750 0.618
    1250.455 0.432 0.444 0.312
    62.50.307 0.301 0.304 0.172
    31.250.228 0.226 0.227 0.095
    00.131 0.133 0.132
  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

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