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  4. Fish trypsin ELISA Kit
  • 中文名稱：
    魚胰蛋白酶(trypsin)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-EL018811FI
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥4200/￥3000
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    魚胰蛋白酶(trypsin)酶聯(lián)免疫試劑盒（CSB-EL018811FI）為競(jìng)爭(zhēng)法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的PRSS1含量。PRSS1是一種編碼陽(yáng)離子胰蛋白酶原的基因，其突變與遺傳性胰腺炎相關(guān)。該基因突變導(dǎo)致胰蛋白酶原激活增加或失活減少，引發(fā)胰腺炎癥。研究顯示，PRSS1突變是慢性胰腺炎的重要遺傳因素，其致病機(jī)制涉及胰蛋白酶的異常激活和胰腺組織的損傷。試劑盒檢測(cè)范圍為0.312 ng/mL-20 ng/mL，可為水產(chǎn)動(dòng)物消化生理研究、疾病模型構(gòu)建及營(yíng)養(yǎng)代謝評(píng)估提供重要指標(biāo)；支持實(shí)驗(yàn)室開(kāi)展魚類消化系統(tǒng)功能研究、水產(chǎn)養(yǎng)殖基礎(chǔ)科研以及環(huán)境因子對(duì)魚類生理影響的毒理學(xué)評(píng)估本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書。
  • 別名：
    Trypsin-1 ELISA kit; EC 3.4.21.4 ELISA kit; Trypsin I ELISA kit
  • 縮寫：
    PRSS1
  • Uniprot No.：
  • 種屬：
    Fish
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    0.312 ng/mL-20 ng/mL
  • 靈敏度：
    0.156 ng/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Cell Biology
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of fish trypsin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:20 Average % 101
    Range % 95-105
    1:40 Average % 91
    Range % 84-95
    1:80 Average % 106
    Range % 100-111
    1:160 Average % 104
    Range % 97-108
  • 回收率：
    The recovery of fish trypsin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 95 87-100
    EDTA plasma (n=4) 102 96-107
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average
    20 0.231 0.227 0.229
    10 0.342 0.337 0.340
    5 0.497 0.482 0.490
    2.5 0.663 0.645 0.654
    1.25 0.932 0.918 0.925
    0.625 1.300 1.246 1.273
    0.312 1.774 1.724 1.749
    0 2.434 2.389 2.412
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 亞細(xì)胞定位：
    Secreted, extracellular space.
  • 蛋白家族：
    Peptidase S1 family
  • 數(shù)據(jù)庫(kù)鏈接：

    KEGG: sasa:100137021

    UniGene: Ssa.38456