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  4. Fish gonadotropin-releasing hormone,GnRH ELISA Kit

Fish gonadotropin-releasing hormone,GnRH ELISA Kit

  • 中文名稱：
    魚類促性腺激素釋放激素(GnRH)酶聯免疫試劑盒
  • 貨號：
    CSB-E08810f
  • 規格：
    96T/48T
  • 價格：
    ￥4800/￥3000
  • 其他：

產品詳情

  • 產品描述：
    魚類促性腺激素釋放激素(GnRH)酶聯免疫試劑盒（CSB-E08810f）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織培養上清液、組織勻漿、細胞裂解物樣本中的GnRH含量。GnRH即促性腺激素釋放激素，是下丘腦分泌的十肽激素。它作用于腺垂體，調節促性腺激素合成與釋放，進而調控性腺功能。研究圍繞其分泌調節機制、與受體相互作用、信號轉導通路等展開，以探索其在生殖內分泌疾病治療中的應用。試劑盒檢測范圍為0.5 ng/mL-20 ng/mL，科研人員可借助本試劑盒開展魚類生殖生理機制研究、水產養殖中人工繁殖技術優化、環境激素干擾評估等實驗，例如監測不同發育階段或環境脅迫下魚類GnRH的動態變化，分析內分泌調控網絡對繁殖行為的影響，為水生生物資源保護及可持續養殖提供數據支持。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 縮寫：
    GnRH
  • Uniprot No.：
  • 種屬：
    Fish
  • 樣本類型：
    serum, plasma, cell culture supernates, tissue homogenates, cell lysates
  • 檢測范圍：
    0.5 ng/mL-20 ng/mL
  • 靈敏度：
    0.5 ng/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Signal Transduction
  • 測定原理：
    quantitative
  • 測定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of fish GnRH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 98  
    Range % 93-104  
    1:2 Average % 94  
    Range % 85-103  
    1:4 Average % 95  
    Range % 83-102  
    1:8 Average % 92  
    Range % 87-101  
  • 回收率：
    The recovery of fish GnRH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 90 87-94  
    EDTA plasma (n=4) 94 89-98  
                 
                 
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average    
    20 0.134 0.140 0.137    
    10 0.232 0.242 0.237    
    4.6 0.398 0.409 0.404    
    1.5 0.714 0.687 0.701    
    0.5 1.293 1.266 1.280    
    0 2.711 2.740 2.726    
  • 數據處理：
  • 貨期：
    3-5 working days

產品評價

相關問答

■ 常見問題解答
Q:

Fish have over a dozen different forms of GnRHs. I would llike to know for which fish GnRH form is this assay designed before placing an order.

A:
Thanks for your inquiry.
This kit is designed against Zebrafish. Pls refer to: http://www.uniprot.org/uniprot/Q5Y835
Q:

If the gene mentioned in the data is indeed gnrh2, the assay cannot be specific for zebrafish or any other fish species. All vertebrate animals, including humans, have gnrh2 gene with mostly unknown function. The GnRH of interest in zebrafish is salmon GnRH or gnrh3 gene. I have to think about it. This kit may not be any better for zebrafish than those for mammalian GnRH because gnrh2 gene encodes for exactly the same functional peptide in all vertebrates.

Do you have data which shows this kit is specific to zebrafish? Could you perhaps send the COA?

A:
Thanks for your inquiry.
GnRH has different isoforms：GnRH，GnRH2 and GnRH3. GnRH-2 is very conservative in different species of fish.
Pls kindly refer to: https://www.nature.com/articles/srep03676.
CSB-E08810f is designed against zebrafish. We tested undiluted serum and plasma samples before and the test value is 0.5--1.5ng/ml.
I will send you COA by email.
Pls let me know if you have any further questions. Thank you.
Q:

Thanks for your response. Based on the literature you provided it seems GnRH2 is not only conserved among fish species but among all vertebrates.
Since I am curious what the difference is between this kit and your kits from other species. Since the antigen is so highly conserved couldn't I use any of your other GnRH kits instead?

A:
Although GnRH2 is highly conserved, different types of animals have different matrix.
Our kit for fish is designed against fish. For different animals, the raw materials used in the kits are different.


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