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Fish Tumor Necrosis Factor α(TNF-α) ELISA KIT

  • 中文名稱:
    魚腫瘤壞死因子α(TNF-α)酶聯免疫試劑盒
  • 貨號:
    CSB-E13254Fh
  • 規格:
    96T/48T
  • 價格:
    ¥4200/¥3000
  • 其他:

產品詳情

  • 產品描述:
    魚腫瘤壞死因子α(TNF-α)酶聯免疫試劑盒(CSB-E13254Fh)為競爭法ELISA試劑盒,定量檢測血清、血漿樣本中的TNF-α含量。TNF-α,即腫瘤壞死因子-α,是一種重要的細胞因子,主要由活化的巨噬細胞產生。它通過與其受體TNFR1結合,激活多種信號通路,影響細胞增殖、凋亡和炎癥反應。TNF-α在多種疾病如炎癥、自身免疫性疾病中起關鍵作用,其研究有助于開發新的治療策略。試劑盒檢測范圍為125 pg/mL-5000 pg/mL,本試劑盒適用于魚類免疫相關研究,如病原感染模型中的炎癥反應監測、疫苗免疫效果評估、水產養殖動物健康狀態分析等科研場景,為水生生物免疫學機制研究及水產疾病防控提供可靠工具。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 縮寫:
    TNF-α
  • Uniprot No.:
  • 種屬:
    Fish
  • 樣本類型:
    serum, plasma
  • 檢測范圍:
    125 pg/mL-5000 pg/mL
  • 靈敏度:
    125 pg/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Immunology
  • 測定原理:
    quantitative
  • 測定方法:
    Competitive
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of fish TNF-α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 95  
    Range % 89-100  
    1:2 Average % 93  
    Range % 87-99  
    1:4 Average % 95  
    Range % 88-103  
    1:8 Average % 96  
    Range % 90-102  
  • 回收率:
    The recovery of fish TNF-α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 99 95-102  
    EDTA plasma (n=4) 95 91-101  
                 
                 
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average    
    5000 0.154 0.156 0.155    
    2500 0.351 0.328 0.340    
    1150 0.562 0.535 0.549    
    375 0.861 0.864 0.863    
    125 1.544 1.530 1.537    
    0 2.387 2.355 2.371    
  • 數據處理:
  • 貨期:
    3-5 working days

引用文獻

產品評價

靶點詳情

  • 功能:
    Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR.
  • 亞細胞定位:
    Membrane; Single-pass type II membrane protein.
  • 蛋白家族:
    Tumor necrosis factor family


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