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Fish Interleukin 6,IL-6 ELISA Kit

  • 中文名稱:
    魚白介素6(IL-6)酶聯(lián)免疫試劑盒
  • 貨號:
    CSB-E13258Fh
  • 規(guī)格:
    96T/48T
  • 價格:
    ¥4200/¥3000
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
    魚白介素6(IL-6)酶聯(lián)免疫試劑盒(CSB-E13258Fh)為競爭法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的IL-6含量。IL-6是重要的炎癥細(xì)胞因子,在免疫調(diào)節(jié)、造血等生理過程中發(fā)揮關(guān)鍵作用。異常升高時與多種疾病如自身免疫病、腫瘤等的發(fā)生發(fā)展密切相關(guān)。其研究機(jī)制主要聚焦于阻斷IL-6與其受體結(jié)合,以抑制下游信號通路從而達(dá)到治療目的。試劑盒檢測范圍為12.5 pg/mL-200 pg/mL,本試劑盒適用于水產(chǎn)養(yǎng)殖中魚類健康監(jiān)測、病原感染模型建立、免疫增強(qiáng)劑效果評估等科研場景,亦可用于比較醫(yī)學(xué)中脊椎動物免疫系統(tǒng)的進(jìn)化機(jī)制探究。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名:
    il6Interleukin-6 ELISA Kit; IL-6 ELISA Kit
  • 縮寫:
    IL-6
  • Uniprot No.:
  • 種屬:
    Fish
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測范圍:
    12.5 pg/mL-200 pg/mL
  • 靈敏度:
    12.5 pg/mL
  • 反應(yīng)時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領(lǐng)域:
    Immunology
  • 測定原理:
    quantitative
  • 測定方法:
    Competitive
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of fish IL-6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 93  
    Range % 88-97  
    1:2 Average % 89  
    Range % 82-94  
    1:4 Average % 103  
    Range % 94-107  
    1:8 Average % 96  
    Range % 90-101  
  • 回收率:
    The recovery of fish IL-6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 100 91-104  
    EDTA plasma (n=4) 91 84-98  
                 
                 
  • 標(biāo)準(zhǔn)曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average    
    200 0.128 0.133 0.131    
    100 0.269 0.258 0.264    
    50 0.449 0.431 0.440    
    25 0.851 0.842 0.847    
    12.5 1.813 1.783 1.798    
  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

引用文獻(xiàn)

產(chǎn)品評價

靶點詳情

  • 功能:
    Cytokine with a wide variety of biological functions in immunity, tissue regeneration, and metabolism. Binds to IL6R, then the complex associates to the signaling subunit IL6ST/gp130 to trigger the intracellular IL6-signaling pathway. The interaction with the membrane-bound IL6R and IL6ST stimulates 'classic signaling', whereas the binding of IL6 and soluble IL6R to IL6ST stimulates 'trans-signaling'. Alternatively, 'cluster signaling' occurs when membrane-bound IL6:IL6R complexes on transmitter cells activate IL6ST receptors on neighboring receiver cells.
  • 亞細(xì)胞定位:
    Secreted.
  • 蛋白家族:
    IL-6 superfamily
  • 組織特異性:
    Expressed in kidney and spleen. Low expression in liver and gills.


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