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  4. Fish Interleukin 1β (IL- 1β) ELISA Kit

Fish Interleukin 1β (IL- 1β) ELISA Kit

  • 中文名稱：
    魚白介素1β(IL-1β)酶聯免疫試劑盒
  • 貨號：
    CSB-E13259Fh
  • 規格：
    96T/48T
  • 價格：
    ￥4200/￥3000
  • 其他：

產品詳情

  • 產品描述：
    魚白介素1β(IL-1β)酶聯免疫試劑盒（CSB-E13259Fh）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的IL1B含量。IL1B是重要的炎癥細胞因子，在多種炎癥性疾病及免疫反應中起關鍵作用。研究機制上，它參與激活免疫細胞、調節炎癥信號通路。圍繞IL1B的研究聚焦于其在疾病發生發展中的作用，為開發抗炎、免疫調節藥物提供靶點。試劑盒檢測范圍為3.12 pg/mL-200 pg/mL，本產品廣泛應用于水產動物免疫學研究、魚類病原感染模型構建、疫苗效力評估以及水產養殖環境脅迫對機體免疫功能影響等科研場景，為深入解析魚類先天免疫機制提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    il1bInterleukin-1 beta ELISA Kit
  • 縮寫：
    IL-1β
  • Uniprot No.：
  • 種屬：
    Fish
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    3.12 pg/mL-200 pg/mL
  • 靈敏度：
    0.78 pg/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Immunology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of fish IL- 1β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 85
    Range % 82-89
    1:2 Average % 94
    Range % 90-99
    1:4 Average % 104
    Range % 97-108
    1:8 Average % 99
    Range % 94-106
  • 回收率：
    The recovery of fish IL- 1β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 95 89-102
    EDTA plasma (n=4) 89 82-95
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    200 2.989 2.778 2.884 2.776
    100 2.467 2.432 2.450 2.342
    50 1.774 1.866 1.820 1.712
    25 0.956 0.932 0.944 0.836
    12.5 0.436 0.456 0.446 0.338
    6.25 0.258 0.270 0.264 0.156
    3.12 0.156 0.155 0.156 0.048
    0 0.105 0.111 0.108 　
  • 數據處理：
  • 貨期：
    3-5 working days

引用文獻

產品評價

靶點詳情

  • 功能：
    Potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production.
  • 基因功能參考文獻：
    1. Major changes due to graded levels of olive leaf extract in the diets were observed in blood biochemical parameters. TNFalpha, IL1-beta and IL-8 gene expressions were significanlty up-regulated in OLE 0.1% group compared with others. PMID: 29733961
    2. The predicted 3D structures of IL-1beta, TNF-alpha, and IL-8 from Oncorhynchus mykiss, Dicentrarchus labrax, and Chionodraco hamatus are reported and the differences between them and their mammalian counterparts investigated. PMID: 21184658
  • 亞細胞定位：
    Cytoplasm, cytosol. Lysosome. Secreted, extracellular exosome. Cytoplasmic vesicle, autophagosome. Secreted.
  • 蛋白家族：
    IL-1 family
  • 組織特異性：
    All three isoforms are expressed in gill, kidney, liver and spleen. Isoform 1 is also expressed in blood. None of the isoforms are expressed in brain.
  • 數據庫鏈接：

    UniGene: Omy.11696



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