Fish Interferon γ,IFN-γ ELISA Kit

產品詳情

文獻引用 (1) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

魚γ干擾素(IFN-γ)酶聯免疫試劑盒（CSB-E12878Fh）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的IFNG含量。干擾素-γ（IFNG）是一種重要的細胞因子，主要由活化的T細胞和NK細胞產生，具有調節免疫狀態、介導細胞免疫、增強吞噬功能等作用。其研究機制涉及JAK-STAT通路，通過激活下游信號級聯，介導多種生物反應，如抗病毒、抗腫瘤和免疫調節。試劑盒檢測范圍為20 pg/mL-4000 pg/mL，適用于水產動物免疫機制研究、感染性疾病模型構建、疫苗效力評估或養殖環境中魚類免疫狀態監測等場景本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

IFN-gamma，interferon gamma
縮寫：

IFN-
種屬：

Fish
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

20 pg/mL-4000 pg/mL
靈敏度：

10 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of fish IFN-γ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	84
	Range %	80-88
1:2	Average %	92
	Range %	87-98
1:4	Average %	100
	Range %	97-103
1:8	Average %	53
	Range %	80-86

回收率：

The recovery of fish IFN-γ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	87-96
EDTA plasma (n=4)	96	90-101

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.