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  4. Fish Insulin-like growth factor 1, IGF-1 ELISA Kit

Fish Insulin-like growth factor 1, IGF-1 ELISA Kit

  • 中文名稱：
    魚胰島素樣生長因子1(IGF-1)酶聯免疫試劑盒
  • 貨號：
    CSB-E12122Fh
  • 規格：
    96T/48T
  • 價格：
    ￥4200/￥3000
  • 其他：

產品詳情

  • 產品描述：
    魚胰島素樣生長因子1(IGF-1)酶聯免疫試劑盒（CSB-E12122Fh）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的IGF-1含量。IGF-1即胰島素樣生長因子-1，它在細胞的生長、分化和增殖等生理過程中發揮重要作用。其研究機制涉及與相應受體結合，激活下游信號通路，影響細胞的存活與功能。目前在生長發育、代謝等多方面研究廣泛，也與某些疾病的發生發展相關。試劑盒檢測范圍為25 pg/mL-2000 pg/mL，可為水產動物生理研究、養殖管理優化以及環境脅迫響應機制探索提供可靠工具，特別適用于實驗室條件下魚類生長性能評估、飼料效應分析以及激素調控網絡研究。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    igf1Insulin-like growth factor I ELISA Kit; IGF-I ELISA Kit; Somatomedin ELISA Kit
  • 縮寫：
    IGF-1
  • Uniprot No.：
  • 種屬：
    Fish
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    25 pg/mL-2000 pg/mL
  • 靈敏度：
    31.25 pg/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Signal Transduction
  • 測定原理：
    quantitative
  • 測定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of fish IGF-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 94  
    Range % 90-98  
    1:2 Average % 95  
    Range % 91-99  
    1:4 Average % 102  
    Range % 98-106  
    1:8 Average % 87  
    Range % 82-93  
  • 回收率：
    The recovery of fish IGF-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 95 90-99  
    EDTA plasma (n=4) 88 82-94  
                 
                 
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average    
    2000 0.341 0.343 0.342    
    750 0.476 0.484 0.480    
    375 0.682 0.636 0.659    
    125 1.067 1.072 1.070    
    25 1.488 1.567 1.528    
    0 1.955 1.995 1.975    
  • 數據處理：
  • 貨期：
    3-5 working days

引用文獻

產品評價

相關問答

■ 常見問題解答
Q:

I want to use Fish Igf-1 ELISA kit for fish's liver homogenate.
In the protocol of the kit, for tissue homogenates, after homogenizing, stored overnight at -20oC. After 2 freeze-thaw cycles, centrifuge the homogenates.I do not understand too much about this. It means I have wait for 2 days to homogenate 2 sample (homogenate-> store -20oC-> thaw-> store 20oC -> thaw-> centrifuge)? Can you explain for me. Thanks!

A:
Thanks for your inquiry.Regarding tissue sample,pls store the sample which has been treated by PBS at -20 degrees overnight.After that,take the sample at room temperature and let it be thawed freely (the first freeze thaw cycle).Then freeze it at -80 degrees or store it at -20 degrees overnight.Then take it at temperature and let it be thawed freely.This is the 2nd freeze-thaw cycle.Regarding the later procedures,pls follow the manual.
If any further questions,pls let me know.
Q:

Can you let me know in which buffer the samples should be diluted?
This is not written in de manual!

A:
Thanks for your inquiry.
CSB-E12122Fh is no need to dilute generally.
So there is no sample diluent included in this kit.Pls let me know if you have any further questions. Thank you.
Q:

In the current protocol nothing is mentioned about an extraction of IGF-1 from binding proteins. This is generally a necessary procedure to properly measure IGF-1. Is an extraction, and if so which, recommended by Cusabio? Is this procedure in the most recent protocol?

A:
Thanks for your inquiry. There is no need to do extraction of IGF-1 from binding proteins for this kit.
Because this kit employs the competitive inhibition enzyme immunoassay technique and detects the total IGF1. Pls let me know if you have any further questions. Thank you.

靶點詳情

  • 功能：
    The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. Acts as a ligand for IGF1R. Binds to the alpha subunit of IGF1R, leading to the activation of the intrinsic tyrosine kinase activity which autophosphorylates tyrosine residues in the beta subunit thus initiatiating a cascade of down-stream signaling events leading to activation of the PI3K-AKT/PKB and the Ras-MAPK pathways. Binds to integrins. Its binding to integrins and subsequent ternary complex formation with integrins and IGFR1 are essential for IGF1 signaling.
  • 基因功能參考文獻：
    1. Nutrient availability, IGF-I, and genetic variation affect weight loss, in part through alterations of proteolytic pathways in rainbow trout. PMID: 19726716
  • 亞細胞定位：
    Secreted.
  • 蛋白家族：
    Insulin family
  • 數據庫鏈接：

    UniGene: Omy.7104



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