Fish Insulin-like growth factor II(IGF2) ELISA kit
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中文名稱:魚胰島素樣生長因子II(IGF2)酶聯免疫試劑盒
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貨號:CSB-EL011088FI
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規格:96T/48T
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價格:¥4200/¥3000
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其他:
產品詳情
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產品描述:魚胰島素樣生長因子II(IGF2)酶聯免疫試劑盒(CSB-EL011088FI)為競爭法ELISA試劑盒,定量檢測血清、血漿樣本中的IGF2含量。IGF2即胰島素樣生長因子2,是一種多功能細胞增殖調控因子,在胚胎生長發育中起重要作用。其研究機制主要圍繞與受體結合激活下游信號通路,影響細胞的增殖、分化和存活,異常表達與多種腫瘤的發生、發展密切相關。試劑盒檢測范圍為62.5 pg/mL-1000 pg/mL,本試劑盒適用于水產養殖研究、魚類生理學實驗、生長調控機制探究等科研領域,特別適用于評估飼料營養配比、環境脅迫對魚類生長的影響或基因工程模型的生物標志物分析。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
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別名:igf2Insulin-like growth factor II ELISA Kit; IGF-II ELISA Kit; Erythrotropin ELISA Kit
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縮寫:
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Uniprot No.:
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種屬:Fish
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樣本類型:serum, plasma
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檢測范圍:62.5 pg/mL-1000 pg/mL
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靈敏度:31.25 pg/mL
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反應時間:1-5h
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樣本體積:50-100ul
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檢測波長:450 nm
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研究領域:Metabolism
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測定原理:quantitative
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測定方法:Competitive
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of fish IGF2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:100 Average % 91 Range % 87-102 1:200 Average % 95 Range % 91-99 1:400 Average % 96 Range % 88-106 1:800 Average % 92 Range % 88-96 -
回收率:
The recovery of fish IGF2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 96 90-102 EDTA plasma (n=4) 93 85-102 -
標準曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. pg/ml OD1 OD2 Average 1000 0.085 0.089 0.087 500 0.192 0.205 0.199 250 0.485 0.489 0.487 125 0.942 0.937 0.940 62.5 1.613 1.572 1.593 0 2.595 2.624 2.610 -
數據處理:
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貨期:3-5 working days
靶點詳情
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功能:The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. Acts as a ligand for integrin which is required for IGF2 signaling.
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亞細胞定位:Secreted.
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蛋白家族:Insulin family
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數據庫鏈接:
UniGene: Omy.11849
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