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Fish Cholecystokinin(CCK) ELISA Kit

  • 中文名稱:
    魚膽囊收縮素/腸促胰酶肽(CCK)酶聯免疫試劑盒
  • 貨號:
    CSB-E13054Fh
  • 規格:
    96T/48T
  • 價格:
    ¥4200/¥3000
  • 其他:

產品詳情

  • 產品描述:
    魚膽囊收縮素/腸促胰酶肽(CCK)酶聯免疫試劑盒(CSB-E13054Fh)為競爭法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的CCK含量。CCK(膽囊收縮素)是一種胃腸道及神經系統分泌的激素,主要作用是刺激膽囊收縮和促進胰腺分泌。其研究機制涉及通過靶細胞表面的受體發揮作用,調節胃腸運動、食欲調節、激素分泌等生理過程。CCK在消化系統和神經系統中的作用密切相關,通過腦腸軸實現雙向調節,影響多種生理和病理過程。試劑盒檢測范圍為200 pg/mL-5000 pg/mL,本試劑盒適用于實驗室環境下的樣本檢測,可應用于水產養殖研究、魚類生理學實驗或環境毒理學評估等場景,為水生生物基礎研究提供高靈敏度的檢測工具。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 縮寫:
    CCK
  • Uniprot No.:
  • 種屬:
    Fish
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測范圍:
    200 pg/mL-5000 pg/mL
  • 靈敏度:
    125 pg/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Metabolism
  • 測定原理:
    quantitative
  • 測定方法:
    Competitive
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<15%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<15%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of fish CCK in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %95
    Range %86-105
    1:2Average %89
    Range %84-96
    1:4Average %96
    Range %91-102
    1:8Average %93
    Range %86-105
  • 回收率:
    The recovery of fish CCK spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 8885-91
    EDTA plasma (n=4)9586-104
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2Average
    50000.137 0.135 0.136
    20000.241 0.228 0.235
    12000.324 0.339 0.332
    5000.616 0.577 0.597
    2001.235 1.274 1.255
    02.602 2.696 2.649
  • 數據處理:
  • 貨期:
    3-5 working days

產品評價

相關問答

 常見問題解答
Q:

Fish CCk ELISA kit cat.numb
Could you give me the specific size parameters of the plate wells? As she will need it for the plate reader machine.

A:
Thanks for your inquiry.
The kit size is 96T and it is 8wells× 12 strips.Pls check the size:
W:8.5cm
L:12.5cm
X:1.25cm
Y:1cm
Generally the microplate reader with 450nm wave can be used. Our company uses the reader from Thermo MK-3.Pls let me know if you have any further questions. Thank you.


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