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  4. Fish (Salmon) leptin (LEP) ELISA kit

Fish (Salmon) leptin (LEP) ELISA kit

  • 中文名稱：
    魚(鮭魚)瘦素(LEP)酶聯免疫試劑盒
  • 貨號：
    CSB-EL012870FI
  • 規格：
    96T/48T
  • 價格：
    ￥4200/￥3000
  • 其他：

產品詳情

  • 產品描述：
    魚(鮭魚)瘦素(LEP)酶聯免疫試劑盒（CSB-EL012870FI）為競爭法ELISA試劑盒，定量檢測血清、血漿樣本中的LEP含量。LEP即瘦素基因編碼蛋白瘦素，其在能量平衡和體重調控中扮演關鍵角色。研究機制主要是，瘦素能作用于下丘腦，通過信號傳導調節食欲、能量消耗等。當下，圍繞LEP在治療肥胖、代謝性疾病等方面的研究不斷開展，以發掘其更多治療潛力。試劑盒檢測范圍為62.5 pg/mL-1000 pg/mL，適用于魚類生理學、營養學及環境毒理學研究，可用于探究餌料成分、溫度變化或污染物暴露對魚類代謝調控的影響，為水產養殖優化和生態保護研究提供可靠工具本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Fish(Salmon)
  • 樣本類型：
    serum, plasma
  • 檢測范圍：
    62.5 pg/mL-1000 pg/mL
  • 靈敏度：
    31.25 pg/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Metabolism
  • 測定原理：
    quantitative
  • 測定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of fish LEP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:20 Average % 91  
    Range % 86-98  
    1:40 Average % 97  
    Range % 91-102  
    1:80 Average % 84  
    Range % 80-87  
    1:160 Average % 90  
    Range % 83-96  
  • 回收率：
    The recovery of fish LEP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 91 87-96  
    EDTA plasma (n=4) 97 93-102  
                 
                 
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average    
    1000 0.082 0.086 0.084    
    500 0.190 0.200 0.195    
    250 0.483 0.444 0.464    
    125 0.945 0.959 0.952    
    62.5 1.672 1.555 1.614    
    0 2.591 2.670 2.631    
  • 數據處理：
  • 貨期：
    3-5 working days

引用文獻

產品評價

相關問答

■ 常見問題解答
Q:

https://www.cusabio.com/ELISA-Kit/Fish-Salmon-leptin-LEP-ELISA-kit-87315.html
Have you ever tested tissue sample?

A:
Thanks for your inquiry!We only tested the mentioned sample stated in the manual. We suggest you follow the manual.If you want to test tissue samplet,we suggest do pre-testing first.Here is the processing way on tissue samples.Pls check for your reference:
Tissue Homogenates 100mg tissue was rinsed with 1X PBS, homogenized in 1 ml of 1X PBS and stored overnight at -20°C. After two freeze-thaw cycles were performed to break the cell membranes, the homogenates were centrifuged for 5 minutes at 5000 x g, 2 - 8°C. The supernate was removed and assayed immediately. Alternatively, aliquot and store samples at -20°C or -80°C. Centrifuge the sample again after thawing before the assay. Avoid repeated freeze-thaw cycles.
Q:

Is this kit also compatible to test Leptin levels in Zebrafish?

A:
Thanks for your inquiry!We suggest you do a pretest if you want to test zebrafish.
Q:

Per customer request, can you please provide the following information about this kit:
a. Capture antibody or capture antigen – immunogen type, sequence (including species source and accession no. if available), expression host, clonality and host species.
b. Detection antibody (if applicable) - immunogen type, sequence (including species source and accession no. if available), expression host, clonality and host species.
c. Standard – if recombinant protein, please specify sequence (including species source and accession no. if available) and expression host.
d. Please send the manual.
e. Please provide your reference data for fish plasma samples.

A:
Thanks for your inquiry.
CSB-EL012870FI
This is a competitive kit. There is only one antibody in the kit.
The plate is coated with mouse monoclonal antibody. ( Immunogen is CHO-derived recombinant protein.）
Standard is CHO-derived recombinant protein.
Pls find attached manual.
We tested salmon serum and plasma and some common freshwater fish before and the test value is about
125pg/ml under 1:100 dilution. The final concentration is about 12.5ng/ml.
Pls let me know if you have any further questions. Thank you.


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