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中文名稱:犬血管緊張素1-7(Ang1-7)酶聯免疫試劑盒
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貨號:CSB-EQ027505DO
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規格:96T/48T
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價格:¥4200/¥3000
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其他:
產品詳情
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產品描述:犬血管緊張素1-7(Ang1-7)酶聯免疫試劑盒(CSB-EQ027505DO)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿、組織培養上清液樣本中的Ang1-7含量。Ang1-7是重要靶點。它屬于腎素-血管緊張素系統成員。其研究機制主要體現在可通過結合Mas受體,發揮舒張血管、降低血壓、抑制炎癥和纖維化等作用,在心血管疾病、腎臟疾病等研究領域有潛在治療價值。試劑盒檢測范圍為6.25 pg/mL-400 pg/mL,研究者可將其應用于犬類心血管疾病模型、腎臟功能研究、代謝綜合征機制探索,以及藥物干預或基因調控對Ang1 - 7通路影響的體外實驗分析,為深入解析RAS系統在病理生理過程中的作用提供可靠工具。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
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縮寫:Ang1-7
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種屬:Canis lupus familiaris (Dog) (Canis familiaris)
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樣本類型:serum, plasma, tissue homogenates, cell culture supernates
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檢測范圍:6.25 pg/mL-400 pg/mL
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靈敏度:1.56 pg/mL
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反應時間:1-5h
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樣本體積:50-100ul
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檢測波長:450 nm
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研究領域:Cardiovascular
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測定原理:quantitative
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測定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of dog Ang1-7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 87 Range % 83-92 1:2 Average % 100 Range % 97-103 1:4 Average % 86 Range % 83-89 1:8 Average % 98 Range % 94-102 -
回收率:
The recovery of dog Ang1-7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 85 81-89 EDTA plasma (n=4) 91 85-94 -
標準曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. pg/ml OD1 OD2 Average Corrected 400 3.061 2.900 2.981 2.871 200 2.541 2.568 2.555 2.445 100 1.751 1.768 1.760 1.650 50 1.027 1.100 1.064 0.954 25 0.505 0.549 0.527 0.417 12.5 0.301 0.294 0.298 0.188 6.25 0.198 0.189 0.194 0.084 0 0.112 0.108 0.110 -
數據處理:
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貨期:3-5 working days
引用文獻
- Effects and Mechanisms of Radiofrequency Ablation of Renal Sympathetic Nerve on Anti-Hypertension in Canine Chen W.et al,Arq Bras Cardiol. ,2017
- Efeitos e Mecanismos da Denerva Chen W.et al,Arq Bras Cardiol.,2017
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