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Dog adrenomedullin,ADM ELISA Kit

  • 中文名稱:
    犬腎上腺髓質素(ADM)酶聯免疫試劑盒
  • 貨號:
    CSB-E09836c
  • 規格:
    96T/48T
  • 價格:
    ¥4200/¥3000
  • 其他:

產品詳情

  • 產品描述:
    犬腎上腺髓質素(ADM)酶聯免疫試劑盒(CSB-E09836c)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿、組織培養上清液樣本中的ADM含量。試劑盒檢測范圍為15.6 pg/mL-1000 pg/mL,適用于體外實驗環境下探索ADM在犬類疾病模型中的調控機制、藥物干預研究或基礎生理功能解析,為動物醫學及比較醫學研究提供可靠工具本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 別名:
    ADM ELISA Kit; ADM [Cleaved into: Adrenomedullin ELISA Kit; AM); Proadrenomedullin N-20 terminal peptide ELISA Kit; ProAM N-terminal 20 peptide ELISA Kit; PAMP ELISA Kit; ProAM-N20)] ELISA Kit
  • 縮寫:
  • Uniprot No.:
  • 種屬:
    Canis lupus familiaris (Dog) (Canis familiaris)
  • 樣本類型:
    serum, plasma, tissue homogenates, cell culture supernates
  • 檢測范圍:
    15.6 pg/mL-1000 pg/mL
  • 靈敏度:
    3.9 pg/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Signal Transduction
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of dog ADM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
     SampleSerum(n=4)
    1:1Average %87
    Range %80-99
    1:2Average %96
    Range %91-104
    1:4Average %98
    Range %90-110
    1:8Average %95
    Range %85-105
  • 回收率:
    The recovery of dog ADM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9582-104
    EDTA plasma (n=4)9790-110
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    10002.675 2.641 2.658 2.502
    5002.159 2.144 2.152 1.996
    2501.312 1.310 1.311 1.155
    1250.778 0.788 0.783 0.627
    62.50.377 0.360 0.369 0.213
    31.250.276 0.264 0.270 0.114
    15.60.190 0.195 0.193 0.037
    00.154 0.158 0.156  
  • 數據處理:
  • 貨期:
    3-5 working days

產品評價

靶點詳情

  • 功能:
    AM and PAMP are potent hypotensive and vasodilatator agents.
  • 亞細胞定位:
    Secreted.
  • 蛋白家族:
    Adrenomedullin family
  • 數據庫鏈接:


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