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  4. Dog Orexin(HCRT) ELISA kit

Dog Orexin(HCRT) ELISA kit

  • 中文名稱：
    犬阿立新(HCRT)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-EL010230DO
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥4200/￥3000
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    犬阿立新(HCRT)酶聯(lián)免疫試劑盒（CSB-EL010230DO）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的HCRT含量。HCRT（食欲素）是一種神經(jīng)肽，在睡眠-覺醒周期調(diào)控等方面發(fā)揮重要作用。其背景方面與維持清醒狀態(tài)相關(guān)。研究機(jī)制上，它通過與特定受體結(jié)合，調(diào)節(jié)神經(jīng)元活動，影響多種生理功能，對其研究有助于深入理解睡眠障礙等相關(guān)疾病。試劑盒檢測范圍為1.87 pg/mL-120 pg/mL，適用于神經(jīng)生物學(xué)、動物行為學(xué)等領(lǐng)域的科學(xué)研究，例如探究犬類睡眠 - 覺醒周期調(diào)控機(jī)制、代謝疾病模型中的HCRT動態(tài)變化，或評估藥物干預(yù)對HCRT信號通路的影響。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    HCRT ELISA Kit; OX ELISA Kit; PPOX ELISA Kit; Orexin ELISA Kit; Hypocretin ELISA Kit; Hcrt) [Cleaved into: Orexin-A ELISA Kit; Hypocretin-1 ELISA Kit; Hcrt1); Orexin-B ELISA Kit; Hypocretin-2 ELISA Kit; Hcrt2)] ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Canis lupus familiaris (Dog) (Canis familiaris)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    1.87 pg/mL-120 pg/mL
  • 靈敏度：
    0.47 pg/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Metabolism
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of dog HCRT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 103
    Range % 96-107
    1:2 Average % 101
    Range % 98-105
    1:4 Average % 94
    Range % 89-98
    1:8 Average % 103
    Range % 96-107
  • 回收率：
    The recovery of dog HCRT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 91 89-95
    EDTA plasma (n=4) 103 98-107
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    120 2.176 2.234 2.205 2.101
    60 1.659 1.765 1.712 1.608
    30 1.193 1.223 1.208 1.104
    15 0.793 0.776 0.785 0.681
    7.5 0.492 0.483 0.488 0.384
    3.75 0.341 0.356 0.349 0.245
    1.87 0.242 0.249 0.246 0.142
    0 0.103 0.104 0.104 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 功能：
    Neuropeptides that play a significant role in the regulation of food intake and sleep-wakefulness, possibly by coordinating the complex behavioral and physiologic responses of these complementary homeostatic functions. A broader role in the homeostatic regulation of energy metabolism, autonomic function, hormonal balance and the regulation of body fluids, is also suggested. Orexin-A binds to both OX1R and OX2R with a high affinity, whereas orexin-B binds only to OX2R with a similar high affinity.
  • 基因功能參考文獻(xiàn)：
    1. The presence of orexin A in the ovary either in dog or in cat is in agreement with previous data on the presence and role of orexins in the female genital system of other species. PMID: 25601132
  • 亞細(xì)胞定位：
    Rough endoplasmic reticulum. Cytoplasmic vesicle. Cell junction, synapse.
  • 蛋白家族：
    Orexin family
  • 數(shù)據(jù)庫鏈接：


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