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Dog Matrix metalloproteinase 3,MMP-3 ELISA kit

  • 中文名稱:
    犬基質金屬蛋白酶3(MMP-3)酶聯免疫試劑盒
  • 貨號:
    CSB-E13621c
  • 規格:
    96T/48T
  • 價格:
    ¥3600/¥2500
  • 其他:

產品詳情

  • 產品描述:
    犬基質金屬蛋白酶3(MMP-3)酶聯免疫試劑盒(CSB-E13621c)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織培養上清液、尿液、組織勻漿樣本中的MMP3含量。MMP3 即基質金屬蛋白酶 3,它在組織重塑、炎癥等生理病理過程中扮演重要角色。研究發現其異常表達與多種疾病如關節炎、腫瘤發展相關。機制上,它可降解細胞外基質成分,影響細胞微環境,促進細胞遷移和腫瘤轉移,是極具潛力的治療靶點。試劑盒檢測范圍為1.56 ng/mL-100 ng/mL,適用于科研場景中犬類生物標志物研究,例如探索炎癥相關疾病的分子機制、評估藥物對組織修復的影響或建立特定病理模型。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 別名:
    MMP3Stromelysin-1 ELISA Kit; SL-1 ELISA Kit; EC 3.4.24.17 ELISA Kit; Matrix metalloproteinase-3 ELISA Kit; MMP-3 ELISA Kit
  • 縮寫:
  • Uniprot No.:
  • 種屬:
    Canis lupus familiaris (Dog) (Canis familiaris)
  • 樣本類型:
    serum, plasma, cell culture supernates, urine, tissue homogenates
  • 檢測范圍:
    1.56 ng/mL-100 ng/mL
  • 靈敏度:
    0.39 ng/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Cancer
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of dog MMP-3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
     SampleSerum(n=4)
    1:1Average %92
    Range %88-96
    1:2Average %89
    Range %85-96
    1:4Average %93
    Range %89-97
    1:8Average %99
    Range %92-105
  • 回收率:
    The recovery of dog MMP-3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9591-99
    EDTA plasma (n=4)9287-96
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    1002.410 2.545 2.478 2.284
    501.960 1.982 1.971 1.777
    251.595 1.563 1.579 1.385
    12.51.177 1.201 1.189 0.995
    6.250.786 0.789 0.788 0.594
    3.120.458 0.462 0.460 0.266
    1.560.278 0.262 0.270 0.076
    00.193 0.195 0.194  
  • 數據處理:
  • 貨期:
    3-5 working days

產品評價

靶點詳情

  • 功能:
    Can degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase.
  • 基因功能參考文獻:
    1. Findings suggested that increased TGF-beta3 and MMP-3 contribute to the pathogenesis of valvular degeneration associated with chronic mitral valve disease. PMID: 21281193
    2. Both mRNA transcript levels and the activities of MMP-3 and MMP-7 are elevated in X-linked Alport syndrome (XLAS) kidneys from dogs. PMID: 15782307
  • 亞細胞定位:
    Secreted, extracellular space, extracellular matrix.
  • 蛋白家族:
    Peptidase M10A family
  • 數據庫鏈接:


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