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Dog Interleukin-33(IL33) ELISA kit

  • 中文名稱:
    犬白介素33(IL33)酶聯免疫試劑盒
  • 貨號:
    CSB-EL011656DO
  • 規格:
    96T/48T
  • 價格:
    ¥4200/¥3000
  • 其他:

產品詳情

  • 產品描述:
    犬白介素33(IL33)酶聯免疫試劑盒(CSB-EL011656DO)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的IL33含量。IL33是重要靶點,其作為白細胞介素家族成員,在免疫調節中起關鍵作用。研究發現它能激活下游信號通路,調控免疫細胞功能。異常表達與哮喘、炎癥性腸病等多種炎癥疾病相關,對其深入研究有助于開發新的免疫治療策略。試劑盒檢測范圍為23.44 pg/mL-1500 pg/mL,本試劑盒可用于探索犬類疾病模型中的免疫調控機制、炎癥相關研究或藥物干預實驗中的生物標志物檢測。其操作流程兼容常規實驗室條件,可為獸醫基礎研究、比較醫學或實驗動物模型開發提供可靠的數據支持,幫助科研人員深入解析IL33在犬類免疫系統中的功能及調控網絡。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 別名:
    IL33Interleukin-33 ELISA Kit; IL-33 ELISA Kit; Protein DVS27 ELISA Kit
  • 縮寫:
  • Uniprot No.:
  • 種屬:
    Canis lupus familiaris (Dog) (Canis familiaris)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測范圍:
    23.44 pg/mL-1500 pg/mL
  • 靈敏度:
    5.86 pg/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Immunology
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of dog IL33 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)
    1:1 Average % 85
    Range % 82-89
    1:2 Average % 102
    Range % 97-107
    1:4 Average % 89
    Range % 85-93
    1:8 Average % 96
    Range % 93-90
  • 回收率:
    The recovery of dog IL33 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 91 88-95
    EDTA plasma (n=4) 97 90-100
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    1500 2.929 2.968 2.949 2.853
    750 2.406 2.300 2.353 2.257
    375 1.631 1.645 1.638 1.542
    187.5 0.915 0.935 0.925 0.829
    93.75 0.506 0.500 0.503 0.407
    46.88 0.254 0.275 0.265 0.169
    23.44 0.141 0.152 0.147 0.051
    0 0.094 0.098 0.096  
  • 數據處理:
  • 貨期:
    3-5 working days

產品評價

靶點詳情

  • 功能:
    Cytokine that binds to and signals through the IL1RL1/ST2 receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells. Involved in the maturation of Th2 cells inducing the secretion of T-helper type 2-associated cytokines. Also involved in activation of mast cells, basophils, eosinophils and natural killer cells. Acts as a chemoattractant for Th2 cells, and may function as an 'alarmin', that amplifies immune responses during tissue injury.; In quiescent endothelia the uncleaved form is constitutively and abundantly expressed, and acts as a chromatin-associated nuclear factor with transcriptional repressor properties, it may sequester nuclear NF-kappaB/RELA, lowering expression of its targets. This form is rapidely lost upon angiogenic or proinflammatory activation.
  • 亞細胞定位:
    Nucleus. Chromosome. Cytoplasm. Cytoplasmic vesicle, secretory vesicle. Secreted.
  • 蛋白家族:
    IL-1 family
  • 組織特異性:
    Expressed in cultured umbilical artery smooth muscle cells after stimulation with IL1A and IL1B, and to a lesser extent with IFNG. Expressed in vasospastic cerebral arteries after subarachnoid hemorrhage.
  • 數據庫鏈接:

    KEGG: cfa:403810

    UniGene: Cfa.3672



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